Effect of Phyllanthus emblica Linn.on Candida adhesion to oral epithelium and denture acrylic

Objective:To investigate the effect of Phyllanthus emblica(P.emblica) Linn,ethanolic extract on the adhesion of Candida albicans(C.albicans) to human buccal epithelial cells(BECs) and denture acrylic surfaces.Methods:Human BECs and transparent acrylic strips were pretreated with ethanolic extract solution of P.emblica fruits at concentration ranged from 18.7 to 300 mg/mL.After washing BECs and the strips were inoculated with three strains of C.albicans (ATCC 10281 and two clinical isolates)(10~7 cells/mL).Normal saline solution(NSS) and 0.2% chlorhexidine gluconate were used as negative and positive controls,respectively.BECs were harvested on 12μm-polycarbonate filters(Millipore,USA).The membrane filters and the strips were stained with Gram stain.Adherent yeast cells on 100 randomly selected epithelial cells and 20 randomly selected fields on each strip were counted under microscope.The statistical significance was calculated by Kruskal-Wallis and Tukey tests at a significant level of P【0.05. Results:Significant lower numbers of all strains of yeasts adhering to BECs and acrylic strips were observed after exposure to 75-300 mg/mL of plant extract compared with NSS.Conclusions: The present study demonstrates that P.emblica ethanolic extract interferes with the adhesion of C. albicans to BECs and denture acrylic surfaces in vitro.

In vitro effects of Salvia officinalis L.essential oil on Candida albicans

Objective:To determine the anticandidal activities of Salvia officinalis L.(S.officinalis)essential oil against Candida albicans(C.albicans)and the inhibitory effects on the adhesion of C.albicans to polymethyl methacrylate(PMMA)resin surface.Methods:Disc diffusion method was first used to test the anticandidal activities of the S.officinalis L.essential oil against the reference strain(ATCC 90028)and 2 clinical strains of C.albicans.Then the minimal inhibitory concentration(MIC)and minimal lethal concentration(MLX)were determined by modified membrane method.The adhesion of C.albicans to PMMA resin surface was assessed after immersion with S.officinalis L.essential oil at various concentrations of 1XMIC.0.5XMIC and 0.25XMIC at room temperature for30 min.One-way ANOVA was used to compare the Candida cell adhesion with the pretreatment agents and Tukey's test was used for multiple comparisons.Results:5.officinalis L.essential oil exhibited anticandidal activity against all strains of C.albicans with inhibition zone ranging from 40.5 mm to 19.5 mm.The MIC and MLC of the oil were determined as 2.780 g/L against all test strains.According to the effects on C.albicans adhesion to PMMA resin surface,it was found that immersion in the essential oil at concentrations of 1XMIC(2.780 g/L).0.5XMIC(1.390 g/L)and0.25XMIC(0.695 g/L)for 30 min significantly reduced the adhesion of all 3 test strains to PMMA resin surface in a dose dependent manner(P<0.05).Conclusions:S.officinalis L.essential oil exhibited anticandidal activities against C.albicans and had inhibitory effects on the adhesion of the cells to PMMA resin surface.With further testing and development,S.officinalis essential oil may be used as an antifungal denture cleanser to prevent candidal adhesion and thus reduce the risk of candida-associated denture stomatitis.

Characterization of the salivary microbiome in healthy Thai children

Objective: To investigate the composition of the salivary microbiome of 50 healthy Thai children. Methods: A total 76 provinces in Thailand are grouped into 5 geographical clusters based on unique economics, foods and lifestyles. Geographical locations and the results of an oral assessment were also considered. Genomic DNA was extracted from stimulated saliva samples. Subsequently, amplicon libraries were prepared by 16 S Metagenomic Sequencing Library Preparation. The amplicons were sequenced using an Illumina Miseq platform followed by bioinformatics and statistical analyses.Results: The correlation between oral hygiene status and caries history varied from r^2=0.887 to r^2=0.999 in the geographical groups, suggesting oral hygiene status a strong association between caries history. Twenty taxonomic groups were found in all subjects and constituted 93.6%-96.5% of the microbiome. Of these, genus Veillonella and Prevotella showed significant differences in their proportions between the geographical groups(P<0.05). Furthermore, the proportion of Veillonella parvula, as well as Rothia aeria and Rothia dentocariosa tended to increase with worse oral hygiene status, which was also related to higher dental caries history.Conclusions: The differences in the salivary microbiome as related to geographic regions suggest that environmental factors, which may include dietary habits, could influence the predominant bacteria found in the mouth of Thai children, especially the genus Veillonella and Prevotella. The ratio of Veillonella parvula, Rothia aeria and Rothia dentocariosa may be indicators of worse oral hygiene status and future caries in this population.

In vitro antimicrobial effects of grape seed extract on peri-implantitis microflora in craniofacial implants

Objective:To determine the antimicrobial effects of grape seed on peri-implantitis microflora.Methods:The grape seed extract was tested against peri-implantitis microflora most commonly found in craniofacial implants including reference strains of Staphylococcus aureus(S.aureus),Escherichia coli(E.coli),Candida albicans(C.albicans)and clinical strains of S.aureus,Klebsiella pneumonia(K.pneumonia)and Candida parapsilosis(C.parapsilosis)by disk diffusion test.Minimum inhibitory concentrations(MIC)and minimum cidal concentrations(MCC)were determined using modified agar dilution millpore method.The extract was further combined with polyethylene glycol and propylene glycol,and was tested for antimicrobial effects.Results:Grape seed extract showed positive inhibitory effects with S.aureus at MIC of 0.625 mg/mL and MCC of1.25 mg/mL respectively.However the extracts showed minimal or no reactivity against strains of E.coli,K.pneumonia,C.parapsilosis and C.albicans.The use of grape seed extract in combination with polyethylene glycol and propylene glycol also showed dose dependent inhibitory effect on S.aureus.Conclusions:The results of the study showed that grape seed has potential antimicrobial effects which can be further studied and developed to be used in the treatment of infected skinabutment interface of craniofacial implants.

Antimicrobial effects of silver zeolite,silver zirconium phosphate silicate and silver zirconium phosphate against oral microorganisms

Objective:To evaluate the antimicrobial activities of silver inorganic materials,including silver zeolite(AgZ),silver zirconium phosphate silicate(AgZrPSi)and silver zirconium phosphate(AgZrp),against oral microorganisms.In line with this objective,the morphology and structure of each type of silver based powders were also investigated.Methods:The antimicrobial activities of AgZ,AgZrPSi and AgZrP were tested against Streptococcus mutans,Lactobacillus casei,Candida albicans and Staphylococcus aureus using disk diffusion assay as a screening test.The minimum inhibitory concentration(MIC)and minimum lethal concentration(MLC)were determined using the modified membrane method.Scanning electron microscope and X-ray diffraction were used to investigate the morphology and structure of these silver materials.Results:All forms of silver inorganic materials could inhibit the growth of all test microorganisms.The MIC of AgZ,AgZrPSi and AgZrP was 10.0 g/L whereas MLC ranged between 10.0-60.0 g/L.In terms of morphology and structure.AgZrPSi and AgZrP had smaller sized particles(1.5-3.0μm)and more uniformly shaped than AgZ.Conclusions:Silver inorganic materials in the form of AgZ,AgZrPSi and AgZrP had antimicrobial effects against all test oral microorganisms and those activities may be influenced by the crystal structure of carriers.These results suggest that these silver materials may be useful metals applied to oral hygiene products to provide antimicrobial activity against oral infection.

Efficacy of xylitol and fluoride mouthrinses on salivary mutans streptococci

Objective:To evaluate the level of salivary Mutatis streptococci(MS) after rinsing with xylitol, fluoride,and a combination of xylitol and fluoride solutions,compared with distilled water. Methods:Eighty healthy 8-9 years old subjects with high level of MS(】 10~5 CFU/mL) were equally divided into 4 groups.Subjects rinsed their moutlis for 1 min with 10 mL of 0.05%(w/v) sodium fluoride(NaF),12.5%(w/v) xylitol or 0.05%(w/v) NoF + 12.5%(w/v) xylitol 3 times daily over 10 weeks.Distilled water rinsed group served as a control.Paraffin-stimulated whole saliva samples were collected at baseline,5 weeks,and 10 weeks after rinsing to determine the level of salivary MS by culturing on Mitis Salivarius Bacitracin agar.The statistical significance was calculated by Kruskol Wallis,Mann Whitney U,and Wilcoxon signed-rank tests at a significant level of P【 0.05.Results:Significant reductions in MS count were observed in subjects using 0.05%NaF + 12.5%xylitol over other groups within 5 weeks and after 10 weeks and 12.5%xylitol alone after 10 weeks compared with baseline.Conclusions:The present study provides evidence for the inhibitory effect of xylitol,used in combination with fluoride,delivered in the form of mouthrinse, on salivary MS in the group of schoolchildren.