Epidemiological surveillance for microbes is currently based on either agar culture followed by identification, or genetic amplification. Both techniques are highly skilled-labor intensive, costly, and must be done in...Epidemiological surveillance for microbes is currently based on either agar culture followed by identification, or genetic amplification. Both techniques are highly skilled-labor intensive, costly, and must be done in central laboratories. The Defined Substrate Utilization®(DSU®) format provides an epidemiological series of specific screening formulations that obviate these limitations. All reagents are present in optimized stable powder form in a test tube—add water, inoculate, and incubate. A specific color change provides a sensitive and specific detection of the target microbe. Two DSU®methods for Staphylococcus aureus (S. aureus) are presented: aureusAlert®for all S. aureus and EPI-M®for methicillin-resistant S. aureus (MRSA). Both aureusAlert®and EPI-M®had a detection level of 20 colony forming units (CFU) in 18 hours. aureusAlert®and conventional methods agreed 93.6% and EPI-M®and conventional methods agreed 94.1%. DSU®and conventional methods showed the same specificity.展开更多
A new, simple, and one-step tool for the direct detection of vancomycin-resistant Enterocococcus (VRE) EPI-V®(Pilots Point LLC, Sarasota, FL) is presented. It contains all the ingredients in a unique stable po...A new, simple, and one-step tool for the direct detection of vancomycin-resistant Enterocococcus (VRE) EPI-V®(Pilots Point LLC, Sarasota, FL) is presented. It contains all the ingredients in a unique stable powder form in a standard test tube. One only needs to add water, inoculate the specimen, and incubate. Specimens consisted of 553 sequential human rectal/perirectal swabs for VRE surveillance. The presence of VRE was denoted by the production of two sequential color changes corresponding to growth in bile-esculin and production of a positive PYR reaction. The EPI-V®tool was compared to reference VRE detection methods. EPI-V®showed a sensitivity of ?102% and a specificity of 98.4% for the detection of VRE. The EPI-V®tool offers significant advantages: no skilled technologist time required, simple quality control, highly conserved incubator and refrigerator space, and low cost.展开更多
文摘Epidemiological surveillance for microbes is currently based on either agar culture followed by identification, or genetic amplification. Both techniques are highly skilled-labor intensive, costly, and must be done in central laboratories. The Defined Substrate Utilization®(DSU®) format provides an epidemiological series of specific screening formulations that obviate these limitations. All reagents are present in optimized stable powder form in a test tube—add water, inoculate, and incubate. A specific color change provides a sensitive and specific detection of the target microbe. Two DSU®methods for Staphylococcus aureus (S. aureus) are presented: aureusAlert®for all S. aureus and EPI-M®for methicillin-resistant S. aureus (MRSA). Both aureusAlert®and EPI-M®had a detection level of 20 colony forming units (CFU) in 18 hours. aureusAlert®and conventional methods agreed 93.6% and EPI-M®and conventional methods agreed 94.1%. DSU®and conventional methods showed the same specificity.
文摘A new, simple, and one-step tool for the direct detection of vancomycin-resistant Enterocococcus (VRE) EPI-V®(Pilots Point LLC, Sarasota, FL) is presented. It contains all the ingredients in a unique stable powder form in a standard test tube. One only needs to add water, inoculate the specimen, and incubate. Specimens consisted of 553 sequential human rectal/perirectal swabs for VRE surveillance. The presence of VRE was denoted by the production of two sequential color changes corresponding to growth in bile-esculin and production of a positive PYR reaction. The EPI-V®tool was compared to reference VRE detection methods. EPI-V®showed a sensitivity of ?102% and a specificity of 98.4% for the detection of VRE. The EPI-V®tool offers significant advantages: no skilled technologist time required, simple quality control, highly conserved incubator and refrigerator space, and low cost.
