大鼠bcl-x_L cDNA的克隆和高滴度重组bcl-x_L腺相关病毒的制备

以RT PCR法从大鼠脑组织中克隆bcl XL 基因 ,将其定向插入带rep cap基因和neu基因的三功能腺相关病毒 (AAV)载体 ,转染HeLa细胞并以G4 18筛选 ,然后用腺病毒感染筛选后的细胞克隆 ,包装成重组腺相关病毒 .用带rep cap基因的C12细胞筛选和滴定产生重组腺相关病毒的细胞克隆 ,粗制细胞裂解物中病毒滴度最高只有 3× 10 5IU ml.从产病毒量较高的克隆大量制备重组病毒 ,经肝素柱高压液相亲和层析法纯化、浓缩病毒后获得了高达 4× 10 11IU ml的重组病毒 .为研究脑缺血动物模型中BCL XL

Modification of ubiquitin C-terminal hydrolase L1 by reactive lipid species: role in neural regeneration and diseases of aging

Role of ubiquitin C-terminal hydrolase L1（UCHL1）in brain function：Ubiquitin is used by a variety of cellular systems to tag proteins for transport to various organelles.There are a number of enzymes in the ubiquitin-proteasome pathway（UPP）that tag abnormally folded proteins with ubiquitin for transport to the proteasome for degradation.