Dear editor,Extraadrenal paragangliomas are tumors composed of neuroendocrine-derived,catecholamine-secreting chromaffin cells inducing hypertension,palpitations,and diaphoresis[1].Although extraadrenal paragangliomas...Dear editor,Extraadrenal paragangliomas are tumors composed of neuroendocrine-derived,catecholamine-secreting chromaffin cells inducing hypertension,palpitations,and diaphoresis[1].Although extraadrenal paragangliomas are found in a variety of anatomic sites that normally host paraganglia—from the carotid body to the abdominal paraaortic region—the bladder is an uncommon location for paragangliomas.Bladder paragangliomas represent approximately 6%of extraadrenal paragangliomas and only 0.05%of all bladder tumors[2,3].We present a rare case of primary paraganglioma affecting the bladder dome.展开更多
Research toward photonic biosensors for point-of-care applications and personalized medicine is driven by the need for high-sensitivity,low-cost,and reliable technology.Among the most sensitive modalities,interferomet...Research toward photonic biosensors for point-of-care applications and personalized medicine is driven by the need for high-sensitivity,low-cost,and reliable technology.Among the most sensitive modalities,interferometry offers particularly high performance,but typically lacks the required operational simplicity and robustness.Here,we introduce a common-path interferometric sensor based on guided-mode resonances to combine high performance with inherent stability.The sensor exploits the simultaneous excitation of two orthogonally polarized modes,and detects the relative phase change caused by biomolecular binding on the sensor surface.The wide dynamic range of the sensor,which is essential for fabrication and angle tolerance,as well as versatility,is controlled by integrating multiple,tuned structures in the field of view.This approach circumvents the trade-off between sensitivity and dynamic range,typical of other phase-sensitive modalities,without increasing complexity.Our sensor enables the challenging label-free detection of procalcitonin,a small protein(13 kDa)and biomarker for infection,at the clinically relevant concentration of 1 pg mL^(−1),with a signal-to-noise ratio of 35.This result indicates the utility for an exemplary application in antibiotic guidance,and opens possibilities for detecting further clinically or environmentally relevant small molecules with an intrinsically simple and robust sensing modality.展开更多
文摘Dear editor,Extraadrenal paragangliomas are tumors composed of neuroendocrine-derived,catecholamine-secreting chromaffin cells inducing hypertension,palpitations,and diaphoresis[1].Although extraadrenal paragangliomas are found in a variety of anatomic sites that normally host paraganglia—from the carotid body to the abdominal paraaortic region—the bladder is an uncommon location for paragangliomas.Bladder paragangliomas represent approximately 6%of extraadrenal paragangliomas and only 0.05%of all bladder tumors[2,3].We present a rare case of primary paraganglioma affecting the bladder dome.
基金financial support from the EPSRC of the United Kingdom(Grants EP/P02324X/1 and EP/P030017/1).Prof.Thomas F.Krauss acknowledges a Royal Society Wolfson Merit Award.Dr.Giampaolo Pitruzzello,Dr.Ben Coulson,Dr.Jose Juan-Colas,Dr.Mostafa Agour,Mr.Ben Kraus,Dr.Kezheng Li,and Dr.Stephen Thorpe contributed technical expertise and fruitful technical discussions.Dr.Ahmad Kenaan shared the PEG spacer part of the functionalization protocol.We also wish to acknowledge Thorlabs UK Ltd.for useful technical advice.
文摘Research toward photonic biosensors for point-of-care applications and personalized medicine is driven by the need for high-sensitivity,low-cost,and reliable technology.Among the most sensitive modalities,interferometry offers particularly high performance,but typically lacks the required operational simplicity and robustness.Here,we introduce a common-path interferometric sensor based on guided-mode resonances to combine high performance with inherent stability.The sensor exploits the simultaneous excitation of two orthogonally polarized modes,and detects the relative phase change caused by biomolecular binding on the sensor surface.The wide dynamic range of the sensor,which is essential for fabrication and angle tolerance,as well as versatility,is controlled by integrating multiple,tuned structures in the field of view.This approach circumvents the trade-off between sensitivity and dynamic range,typical of other phase-sensitive modalities,without increasing complexity.Our sensor enables the challenging label-free detection of procalcitonin,a small protein(13 kDa)and biomarker for infection,at the clinically relevant concentration of 1 pg mL^(−1),with a signal-to-noise ratio of 35.This result indicates the utility for an exemplary application in antibiotic guidance,and opens possibilities for detecting further clinically or environmentally relevant small molecules with an intrinsically simple and robust sensing modality.
