赪桐研究进展及产业化应用展望

赪桐Clerodendrum japonicum作为传统的民间药用植物,可全株入药;耐荫性较好,是林下造景的优良灌木树种,具有潜在药用价值和经济价值。研究表明,赪桐具有较好的抗炎、抗菌、抗补体和抗肿瘤等药效,主要含黄酮类、甾体、香豆素、三萜类等化学成分,并已建立了相应最佳提取工艺。在种苗繁育方面,主要以扦插繁殖为主,可以达到最佳的育苗成效。目前,赪桐分子生物学研究基础较为薄弱,已获得叶绿体基因组和花期全长转录组等序列信息。赪桐由于观赏与药用价值兼备,在林下经济发展、园林绿化及医疗保健方面都有较高的应用价值。文章综述了赪桐药理活性、化学成分、繁殖方法、分子生物学研究、经济价值及其产业化应用等研究进展。

Root morphology and biomass accumulation in hairy root culture of Psammosilene tunicoides by exogenous auxin treatments

Root morphology,growth rate and biomass accumulation in hairy root culture could be impacted by auxins.In this study,we investigated the responses of the morphology and growth of Psammosilene tunicoides hairy roots to different treatments of auxins at several concentrations in solid or liquid media.Results show that Naphthylacetic acid(NAA)principally accelerates the P.tunicoides primary or lateral hairy root elongation,while indolebutyric acid(IBA)mainly increases lateral hairy root density.The biomass of hairy roots was generally promoted by NAA,IBA or NAA/IBA combination.The optimal concentrations of NAA,IBA and NAA/IBA for root biomass enhancements are 0.5,0.1,0.5/0.2 mg/L for solid media,and 0.1,0.1,0.05/0.2 mg/L for liquid media,respectively.This study positively suggests that the root morphology of P.tunicoides hairy root can be improved by optimizing the ratio of NAA/IBA in the culture media,leading to an improving biomass in hairy root culture.