High throughput gene expression analysis is swiftly becoming the focal point for deciphering molecular mechanisms underlying various dif-ferent biological questions. Testament to this is the fact that vast volumes of ...High throughput gene expression analysis is swiftly becoming the focal point for deciphering molecular mechanisms underlying various dif-ferent biological questions. Testament to this is the fact that vast volumes of expression profiles are being generated rapidly by scientists worldwide and subsequently stored in publicly available data repositories such as ArrayEx-press and the Gene Expression Omnibus (GEO). Such wealth of biological data has motivated biologists to compare expression profiles gen-erated from biologically-related microarray ex-periments in order to unravel biological mecha-nisms underlying various states of diseases. However, without the availability of appropriate software and tools, they are compelled to use manual or labour-intensive methods of com-parisons. A scrutiny of current literature makes it apparent that there is a soaring need for such bioinformatics tools that cater for the multiple analyses of expression profiles. In order to contribute towards this need, we have developed an efficient software pipeline for the analysis of multiple gene expression data-sets, called Micropath, which implements three principal functions;1) it searches for common genes amongst n number of datasets using a number crunching method of comparison as well as applying the principle of permutations and combinations in the form of a search strat-egy, 2) it extracts gene expression patterns both graphically and statistically, and 3) it streams co-expressed genes to all molecular pathways belonging to KEGG in a live fashion. We sub-jected MicroPath to several expression datasets generated from our tolerance-related in-house microarray experiments as well as published data and identified a set of 31 candidate genes that were found to be co-expressed across all interesting datasets. Pathway analysis revealed their putative roles in regulating immune toler-ance. MicroPath is freely available to download from: www.1066technologies.co.uk/micropath.展开更多
Objective： The study aimed to explore a lien secure trocar as an effective method to prevent blood spills. Methods： This study included 1200 patients, who had a safety catheter from March to April in 2012, from the ...Objective： The study aimed to explore a lien secure trocar as an effective method to prevent blood spills. Methods： This study included 1200 patients, who had a safety catheter from March to April in 2012, from the emergency department of the Shengjing Hospital of China Medical University. The patients were randomly divided into an experimental group and a control group. Using a conventional method, we compressed the vein at the tip of catheter to prevent blood spillage in the control group. We raised the patient＇s upper limb, while compressing the vein at the tip of catheter in the experimental group. Results： The results showed that the experimental group was better than the control group not only in quantity but also in quality regarding blood pollution. The results reached a level of statistical signifi- cance （P 〈 0.01）. Conclusions： Raising the patient＇s upper limb and occluding the vein distal to the catheter can effectively interrupt blood spillage while withdrawing the steel needle （i.e., pulling out the trocar）.展开更多
文摘High throughput gene expression analysis is swiftly becoming the focal point for deciphering molecular mechanisms underlying various dif-ferent biological questions. Testament to this is the fact that vast volumes of expression profiles are being generated rapidly by scientists worldwide and subsequently stored in publicly available data repositories such as ArrayEx-press and the Gene Expression Omnibus (GEO). Such wealth of biological data has motivated biologists to compare expression profiles gen-erated from biologically-related microarray ex-periments in order to unravel biological mecha-nisms underlying various states of diseases. However, without the availability of appropriate software and tools, they are compelled to use manual or labour-intensive methods of com-parisons. A scrutiny of current literature makes it apparent that there is a soaring need for such bioinformatics tools that cater for the multiple analyses of expression profiles. In order to contribute towards this need, we have developed an efficient software pipeline for the analysis of multiple gene expression data-sets, called Micropath, which implements three principal functions;1) it searches for common genes amongst n number of datasets using a number crunching method of comparison as well as applying the principle of permutations and combinations in the form of a search strat-egy, 2) it extracts gene expression patterns both graphically and statistically, and 3) it streams co-expressed genes to all molecular pathways belonging to KEGG in a live fashion. We sub-jected MicroPath to several expression datasets generated from our tolerance-related in-house microarray experiments as well as published data and identified a set of 31 candidate genes that were found to be co-expressed across all interesting datasets. Pathway analysis revealed their putative roles in regulating immune toler-ance. MicroPath is freely available to download from: www.1066technologies.co.uk/micropath.
文摘Objective： The study aimed to explore a lien secure trocar as an effective method to prevent blood spills. Methods： This study included 1200 patients, who had a safety catheter from March to April in 2012, from the emergency department of the Shengjing Hospital of China Medical University. The patients were randomly divided into an experimental group and a control group. Using a conventional method, we compressed the vein at the tip of catheter to prevent blood spillage in the control group. We raised the patient＇s upper limb, while compressing the vein at the tip of catheter in the experimental group. Results： The results showed that the experimental group was better than the control group not only in quantity but also in quality regarding blood pollution. The results reached a level of statistical signifi- cance （P 〈 0.01）. Conclusions： Raising the patient＇s upper limb and occluding the vein distal to the catheter can effectively interrupt blood spillage while withdrawing the steel needle （i.e., pulling out the trocar）.
