Role of miR-124 in the regulation of retinoic acid-induced Neuro-2A cell differentiation

Retinoic acid can cause many types of cells,including mouse neuroblastoma Neuro-2 A cells,to differentiate into neurons.However,it is still unknown whether microRNAs(miRNAs)play a role in this neuronal differentiation.To address this issue,real-time polymerase chain reaction assays were used to detect the expression of several differentiation-related miRNAs during the differentiation of retinoic acid-treated Neuro-2 A cells.The results revealed that miR-124 and miR-9 were upregulated,while miR-125 b was downregulated in retinoic acid-treated Neuro-2 A cells.To identify the miRNA that may play a key role,miR-124 expression was regulated by transfection of miRNA mimics or inhibitors.Morphological analysis results showed that inhibition of miR-124 expression reversed the effects of retinoic acid on neurite outgrowth.Moreover,miR-124 overexpression alone caused Neuro-2 A cells to differentiate into neurons,and its inhibitor could block this effect.These results suggest that miR-124 plays an important role in retinoic acid-induced differentiation of Neuro-2 A cells.

Generation and characterization of a transgenic mouse model for pancreatic cancer

瞄准：产生转基因的肿瘤动物建模的 SV40Tag 并且学习位于肿瘤发生下面的机制。方法：包含 SV40Tag DNA 的乳腺表示向量被产生。Transgene 碎片是进 FVB 老鼠的受精卵的 microinjeted。遗传上操作的胚胎被变成伪 pregnant 女性老鼠的输卵管。PCR 和北污点分析被用于 F1 和 F2 老鼠的遗传型分析。Transgene 表示被 RT-PCR 和免疫组织化学检测。结果：SV40Tag 基因在转基因的老鼠的二根线被检测。他们之一把 transgene 送到 F1，一个肿瘤在这些老鼠的胰被发现。RT-PCR 和免疫组织化学证明那 SV40Tag 基因在肿瘤被表示。转基因的老鼠的病理学的描述证明肿瘤属于胰腺的膀胱的瘤。结论：SV40Tag 转基因的老鼠模型能成功地被建立。转基因的老鼠得一个胰腺的肿瘤，它能被用于肿瘤发生在活体内的分子的机制的调查。