Cellulose is the utmost plenteous source of biopolymer in our earth,and fungi are the most efcient and ubiquitous organism in degrading the cellulosic biomass by synthesizing cellulases.Tailoring through genetic manip...Cellulose is the utmost plenteous source of biopolymer in our earth,and fungi are the most efcient and ubiquitous organism in degrading the cellulosic biomass by synthesizing cellulases.Tailoring through genetic manipulation has played a substantial role in constructing novel fungal strains towards improved cellulase production of desired traits.However,the traditional methods of genetic manipulation of fungi are time-consuming and tedious.With the availability of the full-genome sequences of several industrially relevant flamentous fungi,CRISPR-CAS(clustered regularly interspaced short palindromic repeats/CRISPR-associated protein)technology has come into the focus for the profcient development of manipulated strains of flamentous fungi.This review summarizes the mode of action of cellulases,transcription level regulation for cellulase expression,various traditional strategies of genetic manipulation with CRISPR-CAS technology to develop modifed fungal strains for a preferred level of cellulase production,and the futuristic trend in this arena of research.展开更多
文摘Cellulose is the utmost plenteous source of biopolymer in our earth,and fungi are the most efcient and ubiquitous organism in degrading the cellulosic biomass by synthesizing cellulases.Tailoring through genetic manipulation has played a substantial role in constructing novel fungal strains towards improved cellulase production of desired traits.However,the traditional methods of genetic manipulation of fungi are time-consuming and tedious.With the availability of the full-genome sequences of several industrially relevant flamentous fungi,CRISPR-CAS(clustered regularly interspaced short palindromic repeats/CRISPR-associated protein)technology has come into the focus for the profcient development of manipulated strains of flamentous fungi.This review summarizes the mode of action of cellulases,transcription level regulation for cellulase expression,various traditional strategies of genetic manipulation with CRISPR-CAS technology to develop modifed fungal strains for a preferred level of cellulase production,and the futuristic trend in this arena of research.