Objective:To investigate the prevalence of Helicobacter pylori(H.pylori)infection in dyspepsia patients and its relation to virulence factor cagA gene.Methods:In total,110 gastric biopsies from dyspeptic patients were...Objective:To investigate the prevalence of Helicobacter pylori(H.pylori)infection in dyspepsia patients and its relation to virulence factor cagA gene.Methods:In total,110 gastric biopsies from dyspeptic patients were comparatively studied using rapid urease test and multiplex polymerase chain reaction(PCR).Results:Multiplex PCR detected three genes of 16S rRNA,cagA,and ureC.H.pylori was detected in 14 gastric biopsies(13%).Significantly higher numbers of female were infected.Furthermore,cag A gene was found in all H.pylori-positive specimens.In addition,the result indicated that the multiplex PCR with annealing temperature at 57℃was able to effectively amplify specific products.Conclusions:The results confirmed high prevalence of cagA gene in H.pylori among dyspeptic patients in Southern Thailand.展开更多
基金Supported by Prince of Songkla University,Research Development Fund,Fiscal Year 2011-2012.
文摘Objective:To investigate the prevalence of Helicobacter pylori(H.pylori)infection in dyspepsia patients and its relation to virulence factor cagA gene.Methods:In total,110 gastric biopsies from dyspeptic patients were comparatively studied using rapid urease test and multiplex polymerase chain reaction(PCR).Results:Multiplex PCR detected three genes of 16S rRNA,cagA,and ureC.H.pylori was detected in 14 gastric biopsies(13%).Significantly higher numbers of female were infected.Furthermore,cag A gene was found in all H.pylori-positive specimens.In addition,the result indicated that the multiplex PCR with annealing temperature at 57℃was able to effectively amplify specific products.Conclusions:The results confirmed high prevalence of cagA gene in H.pylori among dyspeptic patients in Southern Thailand.
