Background and objective Once the malignant pleural or peritoneal effusion is developed it is difficult to control.This report presents a new method for controlling the malignant effusions.Methods Forty-eight patients...Background and objective Once the malignant pleural or peritoneal effusion is developed it is difficult to control.This report presents a new method for controlling the malignant effusions.Methods Forty-eight patients,29 males and 19 females with an average age of 61.2 years old,who were satisfied with the study inclusion criteria,were recruited in this study.Twenty-seven and 21 patients had a malignant pleural and peritoneal effusion,respectively.After draining most of fluids,these patients received intra-cavity infusion of rAd-p53 once per week for 4 weeks,at dose of 2×1012 viral particles(VP) diluted into 200 mL of saline solution for pleural effusions,and 4×1012 VP diluted into 500 mL of saline solution for peritoneal effusions.Results Participants were followed up for a median time of 13.6 month.A total of 11 cases,7 with pleural effusions and 4 with peritoneal effusions achieved a complete response(CR),and 20 cases(12 pleural effusions and 8 peritoneal effusions) had a partial response(PR).The overall response rate is 64.6%.Patients' quality of life,assessed by using Karnofsky performance scale(KPS) scores,was improved by an average of 26.4.The one-year of overall survival rate was 54.2% with a median survival time of 12.5 months.There were no serious side effects observed except for self-limited fever found in 79.8% of the cases.Conclusions Intra-cavity infusion of rAd-p53 is an effective and safe treatment for the patients with malignant pleural or peritoneal effusions,especially for those patients who can't tolerate the standard treatments.展开更多
This study examined the mechanical properties, springback behavior from three-point bending loading–unloading tests and biocompatibility from human osteoblast cell adhesion and proliferation experiments in Ti-15Mo al...This study examined the mechanical properties, springback behavior from three-point bending loading–unloading tests and biocompatibility from human osteoblast cell adhesion and proliferation experiments in Ti-15Mo alloy with different microstructures. The springback ratio increased after the appearance of deformation microstructures including {332} < 113 > twins and dislocations, due to the increased bending strength and unchanged Young’s modulus. By contrast, the change in springback ratio was dependent on the competing effect of the simultaneous increase in bending strength and Young’s modulus after phase transformation, namely, the isothermal ω-phase formation. Good cell adhesion and proliferation were observed on the alloy surface, and they were not significantly affected by the deformation twins, dislocations and isothermal ω-phase.The diversity of deformation and phase transformation microstructures made it possible to control the springback behavior effectively while keeping the biocompatibility of the alloy as an implant rod used for spinal fixation devices.展开更多
An unprecedented gold-catalyzed ketene C=O/C=C bifunctionalization method has been developed.Mechanistic studies and density function theory(DFT)calculations indicate that the reaction is initiated by gold-catalyzed W...An unprecedented gold-catalyzed ketene C=O/C=C bifunctionalization method has been developed.Mechanistic studies and density function theory(DFT)calculations indicate that the reaction is initiated by gold-catalyzed Wolff rearrangement of diazoketone to form the ketene intermediate,followed by intermolecular nucleophilic addition and terminated with two divergent cyclization processes via enol intermediates.In the case with alcohols as the nucleophiles,the reaction goes through a C-5-endodig carbocyclization to give the indene products;whereas,O-7-endo-dig cyclization occurs dominantly when indoles/pyrroles are used as the nucleophiles,delivering the 7-membered benzo[d]oxepines.In comparison with the well-documented cycloaddition and nucleophilic addition reactions,this cascade reaction features a novel reaction pattern for the ketene dual functionalization through addition with nucleophile and electrophile in sequence.展开更多
文摘Background and objective Once the malignant pleural or peritoneal effusion is developed it is difficult to control.This report presents a new method for controlling the malignant effusions.Methods Forty-eight patients,29 males and 19 females with an average age of 61.2 years old,who were satisfied with the study inclusion criteria,were recruited in this study.Twenty-seven and 21 patients had a malignant pleural and peritoneal effusion,respectively.After draining most of fluids,these patients received intra-cavity infusion of rAd-p53 once per week for 4 weeks,at dose of 2×1012 viral particles(VP) diluted into 200 mL of saline solution for pleural effusions,and 4×1012 VP diluted into 500 mL of saline solution for peritoneal effusions.Results Participants were followed up for a median time of 13.6 month.A total of 11 cases,7 with pleural effusions and 4 with peritoneal effusions achieved a complete response(CR),and 20 cases(12 pleural effusions and 8 peritoneal effusions) had a partial response(PR).The overall response rate is 64.6%.Patients' quality of life,assessed by using Karnofsky performance scale(KPS) scores,was improved by an average of 26.4.The one-year of overall survival rate was 54.2% with a median survival time of 12.5 months.There were no serious side effects observed except for self-limited fever found in 79.8% of the cases.Conclusions Intra-cavity infusion of rAd-p53 is an effective and safe treatment for the patients with malignant pleural or peritoneal effusions,especially for those patients who can't tolerate the standard treatments.
基金supported by the National Natural Science Foundation of China(No.52071051)。
文摘This study examined the mechanical properties, springback behavior from three-point bending loading–unloading tests and biocompatibility from human osteoblast cell adhesion and proliferation experiments in Ti-15Mo alloy with different microstructures. The springback ratio increased after the appearance of deformation microstructures including {332} < 113 > twins and dislocations, due to the increased bending strength and unchanged Young’s modulus. By contrast, the change in springback ratio was dependent on the competing effect of the simultaneous increase in bending strength and Young’s modulus after phase transformation, namely, the isothermal ω-phase formation. Good cell adhesion and proliferation were observed on the alloy surface, and they were not significantly affected by the deformation twins, dislocations and isothermal ω-phase.The diversity of deformation and phase transformation microstructures made it possible to control the springback behavior effectively while keeping the biocompatibility of the alloy as an implant rod used for spinal fixation devices.
基金This work was supported by the National Natural Science Foundation of China(21971262,92056201)the National Postdoctoral Program for Innovative Talents(BX20190399)+3 种基金Guangdong Provincial Key Laboratory of Chiral Molecule and Drug Discovery(2019B030301005)the National Mega-project for Innovative Drugs(2019ZX09721001-006-001)the Program for Guangdong Introducing Innovative and Entrepreneurial Teams(2016ZT06Y337)the Fundamental Research Funds for the Central Universities(20ykpy113,18843407).
文摘An unprecedented gold-catalyzed ketene C=O/C=C bifunctionalization method has been developed.Mechanistic studies and density function theory(DFT)calculations indicate that the reaction is initiated by gold-catalyzed Wolff rearrangement of diazoketone to form the ketene intermediate,followed by intermolecular nucleophilic addition and terminated with two divergent cyclization processes via enol intermediates.In the case with alcohols as the nucleophiles,the reaction goes through a C-5-endodig carbocyclization to give the indene products;whereas,O-7-endo-dig cyclization occurs dominantly when indoles/pyrroles are used as the nucleophiles,delivering the 7-membered benzo[d]oxepines.In comparison with the well-documented cycloaddition and nucleophilic addition reactions,this cascade reaction features a novel reaction pattern for the ketene dual functionalization through addition with nucleophile and electrophile in sequence.
