The use of nanotechnology in nanoparticle-based cancer therapeutics is gaining impetus due to the unique biophysical properties of nanoparticles at the quantum level. Silver nanoparticles （AgNPs） have been reported ...The use of nanotechnology in nanoparticle-based cancer therapeutics is gaining impetus due to the unique biophysical properties of nanoparticles at the quantum level. Silver nanoparticles （AgNPs） have been reported as one type of potent therapeutic nanoparticles. The present study is aimed to determine the effect of AgNPs in arresting the growth of a murine fibrosarcoma by a reductive mechanism. Initially, a bioavailability study showed that mouse serum albumin （MSA）-coated AgNPs have enhanced uptake; therefore, toxicity studies of AgNP-MSA at 10 different doses （1-10 mg/kg b.w.） were performed in LACA mice by measuring the complete blood count, lipid profile and histological parameters. The complete blood count, lipid profile and histological parameter results showed that the doses from 2 to 8 mg （IC50： 6.15 mg/kg b.w.） sequentially increased the count of leukocytes, lymphocytes and granulocytes, whereas the 9- and 10-mg doses showed conclusive toxicity. In an antitumor study, the incidence and size of fibrosarcoma were reduced or delayed when murine fibrosarcoma groups were treated by AgNP-MSA. Transmission electron micrographs showed that considerable uptake of AgNP-MSA by the sentinel immune cells associated with tumor tissue and a morphologically buckled structure of the immune cells containing AgNP-MSA. Because the toxicity studies revealed a relationship between AgNPs and immune function, the protumorigenic cytokines TNF-α, IL-6 and IL-1β were also assayed in AgNP-MSA-treated and non-treated fibrosarcoma groups, and these cytokines were found to be downregulated after treatment with AgNP-MSA.展开更多
The use of the optoelectronic properties of noble metal nanoparticles has given a new dimension to the realm of cancer diagnosis and therapy(theranostics).The multifunctional catalytic properties and surface plasmon r...The use of the optoelectronic properties of noble metal nanoparticles has given a new dimension to the realm of cancer diagnosis and therapy(theranostics).The multifunctional catalytic properties and surface plasmon resonance of noble metal nanoparticles(NPs)along with their facile surface chemistry gives them an edge in the field of biology,including for immunotherapeutic applications.In a previous study,we reported the anticancer efficacy of silver NPs coated with murine serum albumin against fibrosarcoma in an in vivo mouse model,highlighting their role in eliciting oxystress and immune interference.In addition to addressing the bioavailability and toxicity parameters,our study reported that there was a significant reduction in the size,as well as a delay in the incidence of tumors with AgNP-MSA treatment.Transmission electron micrographs revealed AgNP-MSA uptake by sentinel immune cells associated with the tumor along with the downregulation of pro-tumorigenic inflammatory cytokines,such as TNF-α,IL-6 and IL-1β.展开更多
文摘The use of nanotechnology in nanoparticle-based cancer therapeutics is gaining impetus due to the unique biophysical properties of nanoparticles at the quantum level. Silver nanoparticles （AgNPs） have been reported as one type of potent therapeutic nanoparticles. The present study is aimed to determine the effect of AgNPs in arresting the growth of a murine fibrosarcoma by a reductive mechanism. Initially, a bioavailability study showed that mouse serum albumin （MSA）-coated AgNPs have enhanced uptake; therefore, toxicity studies of AgNP-MSA at 10 different doses （1-10 mg/kg b.w.） were performed in LACA mice by measuring the complete blood count, lipid profile and histological parameters. The complete blood count, lipid profile and histological parameter results showed that the doses from 2 to 8 mg （IC50： 6.15 mg/kg b.w.） sequentially increased the count of leukocytes, lymphocytes and granulocytes, whereas the 9- and 10-mg doses showed conclusive toxicity. In an antitumor study, the incidence and size of fibrosarcoma were reduced or delayed when murine fibrosarcoma groups were treated by AgNP-MSA. Transmission electron micrographs showed that considerable uptake of AgNP-MSA by the sentinel immune cells associated with tumor tissue and a morphologically buckled structure of the immune cells containing AgNP-MSA. Because the toxicity studies revealed a relationship between AgNPs and immune function, the protumorigenic cytokines TNF-α, IL-6 and IL-1β were also assayed in AgNP-MSA-treated and non-treated fibrosarcoma groups, and these cytokines were found to be downregulated after treatment with AgNP-MSA.
文摘The use of the optoelectronic properties of noble metal nanoparticles has given a new dimension to the realm of cancer diagnosis and therapy(theranostics).The multifunctional catalytic properties and surface plasmon resonance of noble metal nanoparticles(NPs)along with their facile surface chemistry gives them an edge in the field of biology,including for immunotherapeutic applications.In a previous study,we reported the anticancer efficacy of silver NPs coated with murine serum albumin against fibrosarcoma in an in vivo mouse model,highlighting their role in eliciting oxystress and immune interference.In addition to addressing the bioavailability and toxicity parameters,our study reported that there was a significant reduction in the size,as well as a delay in the incidence of tumors with AgNP-MSA treatment.Transmission electron micrographs revealed AgNP-MSA uptake by sentinel immune cells associated with the tumor along with the downregulation of pro-tumorigenic inflammatory cytokines,such as TNF-α,IL-6 and IL-1β.
