Extracts of two edible mushrooms, Pleurotus sajor-caju (commercial) and Schizophyllum commune (wild) were used to compare the antioxidant and antimicrobial properties. Aqueous and three types of organic solvents, like...Extracts of two edible mushrooms, Pleurotus sajor-caju (commercial) and Schizophyllum commune (wild) were used to compare the antioxidant and antimicrobial properties. Aqueous and three types of organic solvents, like 50% of ethanol, methanol and acetone extracts were used in trial. DPPH scavenging activity in P. sajor-caju extract was determined in the range of 53.13% to 85.08%, whereas extracts of S. commune were observed in the range of 54.11% to 97.19% at a concentration of 5 mg/ml. The highest DPPH scavenging activity of 97.19% was observed in ethanol extract of S. commune (97.19%), higher than butyl hydroxytoluene (BTH). Half effective concentration (EC<sub>50</sub>) in extracts of P. sajor-caju was found in the range of 1.47 to 4.23 mg/ml and that of S. commune in the range of 1.52 to 4.52 mg/ml. The reducing power of P. sajor caju aqueous concentration extract was found to be the closest of 3.353 (700 nm) that of antioxidant activity to BHT (3.445) at 2 mg/ml concentration. The best reducing power EC<sub>50</sub> was obtained in P. sajor caju aqueous extract (0.09 mg/ml), but in S. commune with acetone extract (0.22 mg/ml). Minimum inhibition concentration (MIC) was compared in extracts of mushrooms in various Vibrio species. All extracts were able to inhibit V. harveyi growth with MIC of lower than 1.25 mg/ml. In aqueous and methanol extracts of current study showed that bacteria inhibition activity occurred at the concentration of P. sajor-caju was able to act as reducing agent as functional as the commercial antioxidant agent, BHT. Crude extracts of P. sajor-caju and S. commune were observed to contain antibacterial potential against these mentioned Vibrio bacteria.展开更多
Afifella marina strain ME (KC205142), a purple non-sulfur bacterium was isolated from mangrove habitats of Sabah. The effects of light intensities and photoperiods on proteolytic activity in Afifella marina strain ME ...Afifella marina strain ME (KC205142), a purple non-sulfur bacterium was isolated from mangrove habitats of Sabah. The effects of light intensities and photoperiods on proteolytic activity in Afifella marina strain ME (KC205142) were investigated. Secretion of proteolytic enzymes in Afifella marina was preliminarily assessed by skim milk agarose media. Subsequently, light intensities, such as, dark, 1000, 1500, 2000, 2500, 3000, 3500, 4000, 4500 and 5000 lux were used to evaluate the effects on proteolytic activity in Afifella marina strain ME under anaerobic condition. After that, the effect of photoperiods on proteolytic activity was monitored under anaerobic light condition (3000 lux) at 0 h (0L/24D), 6 h (6L/18D), 12 h (12L/12D), 18 h (18L/6D) and 24 h (24L/0D) of photoperiod. The highest proteolytic activity of 74.67 U was recorded at 3000 lux illumination light intensity. The proteolytic activity in bacterium Afifella marina strain ME was positively associated with the dry cell weight. The proteolytic activity of 72.67 U in bacterium Afifella marina strain ME at 18 h (18L/6D) photoperiod is not significantly different (p > 0.05) from proteolytic activity of 74.67 U recorded at continuous light (24L/0D) condition. Light intensity of 3000 lux, culture period of 48 h and a photoperiod of 18 h (18L/ 6D) were the optimum parameters for proteolytic activity in bacterium Afifella marina strain ME.展开更多
文摘Extracts of two edible mushrooms, Pleurotus sajor-caju (commercial) and Schizophyllum commune (wild) were used to compare the antioxidant and antimicrobial properties. Aqueous and three types of organic solvents, like 50% of ethanol, methanol and acetone extracts were used in trial. DPPH scavenging activity in P. sajor-caju extract was determined in the range of 53.13% to 85.08%, whereas extracts of S. commune were observed in the range of 54.11% to 97.19% at a concentration of 5 mg/ml. The highest DPPH scavenging activity of 97.19% was observed in ethanol extract of S. commune (97.19%), higher than butyl hydroxytoluene (BTH). Half effective concentration (EC<sub>50</sub>) in extracts of P. sajor-caju was found in the range of 1.47 to 4.23 mg/ml and that of S. commune in the range of 1.52 to 4.52 mg/ml. The reducing power of P. sajor caju aqueous concentration extract was found to be the closest of 3.353 (700 nm) that of antioxidant activity to BHT (3.445) at 2 mg/ml concentration. The best reducing power EC<sub>50</sub> was obtained in P. sajor caju aqueous extract (0.09 mg/ml), but in S. commune with acetone extract (0.22 mg/ml). Minimum inhibition concentration (MIC) was compared in extracts of mushrooms in various Vibrio species. All extracts were able to inhibit V. harveyi growth with MIC of lower than 1.25 mg/ml. In aqueous and methanol extracts of current study showed that bacteria inhibition activity occurred at the concentration of P. sajor-caju was able to act as reducing agent as functional as the commercial antioxidant agent, BHT. Crude extracts of P. sajor-caju and S. commune were observed to contain antibacterial potential against these mentioned Vibrio bacteria.
文摘Afifella marina strain ME (KC205142), a purple non-sulfur bacterium was isolated from mangrove habitats of Sabah. The effects of light intensities and photoperiods on proteolytic activity in Afifella marina strain ME (KC205142) were investigated. Secretion of proteolytic enzymes in Afifella marina was preliminarily assessed by skim milk agarose media. Subsequently, light intensities, such as, dark, 1000, 1500, 2000, 2500, 3000, 3500, 4000, 4500 and 5000 lux were used to evaluate the effects on proteolytic activity in Afifella marina strain ME under anaerobic condition. After that, the effect of photoperiods on proteolytic activity was monitored under anaerobic light condition (3000 lux) at 0 h (0L/24D), 6 h (6L/18D), 12 h (12L/12D), 18 h (18L/6D) and 24 h (24L/0D) of photoperiod. The highest proteolytic activity of 74.67 U was recorded at 3000 lux illumination light intensity. The proteolytic activity in bacterium Afifella marina strain ME was positively associated with the dry cell weight. The proteolytic activity of 72.67 U in bacterium Afifella marina strain ME at 18 h (18L/6D) photoperiod is not significantly different (p > 0.05) from proteolytic activity of 74.67 U recorded at continuous light (24L/0D) condition. Light intensity of 3000 lux, culture period of 48 h and a photoperiod of 18 h (18L/ 6D) were the optimum parameters for proteolytic activity in bacterium Afifella marina strain ME.
