After harvest,cassava(Manihot esculenta Crantz)storage roots undergo rapid postharvest physiological deterioration,producing blue-brown discoloration in the vasculature due to the production of polyphenolics(mainly qu...After harvest,cassava(Manihot esculenta Crantz)storage roots undergo rapid postharvest physiological deterioration,producing blue-brown discoloration in the vasculature due to the production of polyphenolics(mainly quinonesand coumarins)by enzymes such as polyphenol oxidase(PPO).Here,we report the application of hen egg-white lysozyme(HEWL),a natural PPO inhibitor,in transgenic cassava to repress the symptoms of postharvest physiological deterioration.The HEWL-expressing transgenic plants had lower levels of the two main cassava coumarins tested,scopoletin and scopolin,compared with wild type.HEWL-expressing cassava also showed increased tolerance of oxidative stress.Overall,the lysozyme-PPO system proved to be functional in plants for repressing PPO-mediated commercial product browning.展开更多
基金supported by the National Key R&D Program of China(2018YFD1000500)China Agriculture Research System(CARS-11)。
文摘After harvest,cassava(Manihot esculenta Crantz)storage roots undergo rapid postharvest physiological deterioration,producing blue-brown discoloration in the vasculature due to the production of polyphenolics(mainly quinonesand coumarins)by enzymes such as polyphenol oxidase(PPO).Here,we report the application of hen egg-white lysozyme(HEWL),a natural PPO inhibitor,in transgenic cassava to repress the symptoms of postharvest physiological deterioration.The HEWL-expressing transgenic plants had lower levels of the two main cassava coumarins tested,scopoletin and scopolin,compared with wild type.HEWL-expressing cassava also showed increased tolerance of oxidative stress.Overall,the lysozyme-PPO system proved to be functional in plants for repressing PPO-mediated commercial product browning.
