The effect of nitrogen(N)fertilizer on the development of maize kernels has yet to be fully explored.MicroRNA-mRNA analyses could help advance our understanding of how kernels respond to N.This study analyzed the morp...The effect of nitrogen(N)fertilizer on the development of maize kernels has yet to be fully explored.MicroRNA-mRNA analyses could help advance our understanding of how kernels respond to N.This study analyzed the morphological,physiological,and transcriptomic changes in maize kernels under different N rates(0,100,200,and 300 kg ha–1).The result showed that increasing N application significantly increased maize grains’fresh and dry weight until N reached 200 kg ha–1.Higher levels of indole-3-acetic acid,cytokinin,gibberellin,and a lower level of ethylene were associated with increased N applications.We obtained 31 differentially expressed genes(DEGs)in hormone synthesis and transduction,and 9 DEGs were regulated by 14 differentially expressed microRNAs(DEMIs)in 26 pairs.The candidate DEGs and DEMIs provide valuable insight for manipulating grain filling under different N rates.展开更多
Cotton is the main economically important crop in Xinjiang,China,but soil salinization and shortage of water and nutrients have restricted its production.A field experiment was carried out in the salinity-affected ari...Cotton is the main economically important crop in Xinjiang,China,but soil salinization and shortage of water and nutrients have restricted its production.A field experiment was carried out in the salinity-affected arid area of Northwest China from 2018 to 2019 to explore the effects of nitrogen and water regulation on physiological growth,yield,water and nitrogen use efficiencies,and economic benefit of cotton.The salinity levels were 7.7(SL)and 12.5 dS/m(SM).Drip irrigation was used with low,medium and adequate irrigation levels representing 60%,80%and 100%of cotton crop water demand,respectively,and three nitrogen applications,i.e.,206,275 and 343 kg/hm^(2),accounting for 75%,100%and 125%of local N application,respectively were used.The multi-objective optimization based on spatial analysis showed that,at SL salinity,water use efficiency(WUE),nitrogen use efficiency(NUE),economic benefit and yield simultaneously reached more than 85%of their maxima at 379.18-398.32 mm irrigation and 256.69-308.87 kg/hm^(2).At SM salinity,WUE,yield and economic benefit simultaneously reached more than 85%of their maxima when irrigation was 351.24-376.30 mm and nitrogen application was 230.18-289.89 kg/hm^(2).NUE,yield and economic benefit simultaneously reached their maxima at 428.01-337.72 mm irrigation,and nitrogen application range was 222.14-293.93 kg/hm^(2).The plants at SL salinity had 21.58%-46.59%higher WUE rates,14.91%-34.35%higher NUE rates and 20.71%-35.34%higher yields than those at SM salinity.The results are of great importance for the nutrient and water management in cotton field in the arid saline area.展开更多
基金supported by the Major Special Research Projects in Gansu Province,China(22ZD6NA009)the State Key Laboratory of Aridland Crop Science,Gansu Agricultural University,China(GSCS-2022-Z02)+1 种基金the National Natural Science Foundation of China(32260549)the National Key R&D Program of China(2022YFD1900300)。
文摘The effect of nitrogen(N)fertilizer on the development of maize kernels has yet to be fully explored.MicroRNA-mRNA analyses could help advance our understanding of how kernels respond to N.This study analyzed the morphological,physiological,and transcriptomic changes in maize kernels under different N rates(0,100,200,and 300 kg ha–1).The result showed that increasing N application significantly increased maize grains’fresh and dry weight until N reached 200 kg ha–1.Higher levels of indole-3-acetic acid,cytokinin,gibberellin,and a lower level of ethylene were associated with increased N applications.We obtained 31 differentially expressed genes(DEGs)in hormone synthesis and transduction,and 9 DEGs were regulated by 14 differentially expressed microRNAs(DEMIs)in 26 pairs.The candidate DEGs and DEMIs provide valuable insight for manipulating grain filling under different N rates.
基金The study was supported by the National Natural Science Foundation of China(U1803244,51669029,2020DB01)the National Key Research and Development Program of China(2016YFC0501406).
文摘Cotton is the main economically important crop in Xinjiang,China,but soil salinization and shortage of water and nutrients have restricted its production.A field experiment was carried out in the salinity-affected arid area of Northwest China from 2018 to 2019 to explore the effects of nitrogen and water regulation on physiological growth,yield,water and nitrogen use efficiencies,and economic benefit of cotton.The salinity levels were 7.7(SL)and 12.5 dS/m(SM).Drip irrigation was used with low,medium and adequate irrigation levels representing 60%,80%and 100%of cotton crop water demand,respectively,and three nitrogen applications,i.e.,206,275 and 343 kg/hm^(2),accounting for 75%,100%and 125%of local N application,respectively were used.The multi-objective optimization based on spatial analysis showed that,at SL salinity,water use efficiency(WUE),nitrogen use efficiency(NUE),economic benefit and yield simultaneously reached more than 85%of their maxima at 379.18-398.32 mm irrigation and 256.69-308.87 kg/hm^(2).At SM salinity,WUE,yield and economic benefit simultaneously reached more than 85%of their maxima when irrigation was 351.24-376.30 mm and nitrogen application was 230.18-289.89 kg/hm^(2).NUE,yield and economic benefit simultaneously reached their maxima at 428.01-337.72 mm irrigation,and nitrogen application range was 222.14-293.93 kg/hm^(2).The plants at SL salinity had 21.58%-46.59%higher WUE rates,14.91%-34.35%higher NUE rates and 20.71%-35.34%higher yields than those at SM salinity.The results are of great importance for the nutrient and water management in cotton field in the arid saline area.