Generation of mouse models carrying a defined point mutation,especially disease-related point mutations,is of considerable interest for research in biology and medicine.The standard method based on embryonic stem cell...Generation of mouse models carrying a defined point mutation,especially disease-related point mutations,is of considerable interest for research in biology and medicine.The standard method based on embryonic stem cell（ESC）-mediated homologous recombination（HR）is time-and labor-consuming.展开更多
基金supported by the Ministry of Science and Technology of China (2014CB964803 and 2015AA020307)the National Natural Science Foundation of China (Nos. 31530048, 31601163 and 81672117)+1 种基金he Chinese Academy of Sciences (XDB19010204 and QYZDJ-SSW-SMC023)the Shanghai Municipal Commission for Science and Technology(16JC1420500, 17JC1400900 and 17140901500)
文摘Generation of mouse models carrying a defined point mutation,especially disease-related point mutations,is of considerable interest for research in biology and medicine.The standard method based on embryonic stem cell（ESC）-mediated homologous recombination（HR）is time-and labor-consuming.
