Based on the study for the effects of the viscosity of suspending medium upon RBC deformability with Ektacytometry,we further researched the influence of the viscosity of suspending medium upon the orientation and def...Based on the study for the effects of the viscosity of suspending medium upon RBC deformability with Ektacytometry,we further researched the influence of the viscosity of suspending medium upon the orientation and deformatioin of RBCs in the orbit"C=0"with a new Ektacytometry developed by ourselves.The results of the study show that the number of RBCs in the orbit"C=O"was obviously reduced due to the un-stability of the flow field of low viscosity at high shear rate region.So the precision for detecting the orientation index(DI)or and the deformation index(DI)a was seriously affected.On the other hand,(DI)。will obviously decrease due to the resistant effect of viscous force of suspending medium on RBCs in a shear flow field.Meantime,the de-formation of RBCs in the orbit"C=0"rises and falls slightly in a shear flow field of low viscosity and the deformation in the orbit"C=π/2"(tank treading motion)increas-es with the viscosity of suspending medium in a shear flow field of high viscosity.In this paper,through studying of the viscosity rfsuspending medium on the orientation and the deformation of RBCs,we will experime...lly give a optimum viscosity of suspending medium for the new Ektacytometry to obtain both(DI)or and(DI).for the best precision and will get a new Ektacytometry based on reliable theory and practice.展开更多
After injecting VP16, MEL cells and MEL-TF19 cells into the body of mice, with those injected with the same dose of saline as the control group, we observed the mice for their blood pictures, histological changes of t...After injecting VP16, MEL cells and MEL-TF19 cells into the body of mice, with those injected with the same dose of saline as the control group, we observed the mice for their blood pictures, histological changes of the liver and spleen, and the hemorhelogical indexes within 4 weeks. The results indicated that after injecting MEL cells, the mice entered into a pathological status similar to erythroleukemia, which had the following exhibitions: the tissue structures of the liver and spleen were damaged, a mass of proerythroblasts, basophil erythroblasts and polychromatophilic erythroblasts could be observed on the smears of the bone marrow and spleen, and the deformability and orientation ability of erythrocytes were both depressed. The pathogenicity of MEL-TF19 cells carrying TFAR19 gene was obviously lower than that of MEL cells, and the MEL-TF19 cells even lost their faintish pathogenicity under the apop-tosis-inducing effect of the chemotherapeutic reagent. The outcome from the animal experiments suggests that the TFAR19 gene suppresses the pathogenicity of MEL cells to the mice, and the effect may be better exerted with the synergy of the chemotherapeutic reagent.展开更多
文摘Based on the study for the effects of the viscosity of suspending medium upon RBC deformability with Ektacytometry,we further researched the influence of the viscosity of suspending medium upon the orientation and deformatioin of RBCs in the orbit"C=0"with a new Ektacytometry developed by ourselves.The results of the study show that the number of RBCs in the orbit"C=O"was obviously reduced due to the un-stability of the flow field of low viscosity at high shear rate region.So the precision for detecting the orientation index(DI)or and the deformation index(DI)a was seriously affected.On the other hand,(DI)。will obviously decrease due to the resistant effect of viscous force of suspending medium on RBCs in a shear flow field.Meantime,the de-formation of RBCs in the orbit"C=0"rises and falls slightly in a shear flow field of low viscosity and the deformation in the orbit"C=π/2"(tank treading motion)increas-es with the viscosity of suspending medium in a shear flow field of high viscosity.In this paper,through studying of the viscosity rfsuspending medium on the orientation and the deformation of RBCs,we will experime...lly give a optimum viscosity of suspending medium for the new Ektacytometry to obtain both(DI)or and(DI).for the best precision and will get a new Ektacytometry based on reliable theory and practice.
基金the National Natural Science Foundation of China (Grant Nos. 30270355 & 10572007)
文摘After injecting VP16, MEL cells and MEL-TF19 cells into the body of mice, with those injected with the same dose of saline as the control group, we observed the mice for their blood pictures, histological changes of the liver and spleen, and the hemorhelogical indexes within 4 weeks. The results indicated that after injecting MEL cells, the mice entered into a pathological status similar to erythroleukemia, which had the following exhibitions: the tissue structures of the liver and spleen were damaged, a mass of proerythroblasts, basophil erythroblasts and polychromatophilic erythroblasts could be observed on the smears of the bone marrow and spleen, and the deformability and orientation ability of erythrocytes were both depressed. The pathogenicity of MEL-TF19 cells carrying TFAR19 gene was obviously lower than that of MEL cells, and the MEL-TF19 cells even lost their faintish pathogenicity under the apop-tosis-inducing effect of the chemotherapeutic reagent. The outcome from the animal experiments suggests that the TFAR19 gene suppresses the pathogenicity of MEL cells to the mice, and the effect may be better exerted with the synergy of the chemotherapeutic reagent.
