The process of economic activities is on the basis of tremendous material inputs.China has been discharging an enormous amount of waste,giving rise to a wide range of environmental impacts.The method of economy-wide m...The process of economic activities is on the basis of tremendous material inputs.China has been discharging an enormous amount of waste,giving rise to a wide range of environmental impacts.The method of economy-wide material flow analysis(EW-MFA)is one of the effective tools to examine the flow of materials entering physical economies,and recognize early environmental problems.Relevant researches are still at the early stage in China and most focus on material throughput but are rarely concerned about the utilization of recycling resource and environmental impact.Based on more than 3,000 items of data related,materials entering Chinese economy are classified into three types,and then the characteristics of material input and environmental impact are presented for the years 1990-2005 by using the indicators derived from EW-MFA.The Ratio of Recycled Material(RRM) is added as the new indicator in order to be in accordance with the need of circular economy being promoted in China.Results show that the great changes in the structure of material input cause the continuous increase of industrial solid waste emissions and the bogging down of material productivity.The RRM reveals that the utilization of recycling resources remains at a fairly low level in China.Finally,some weakness of EW-MFA is discussed according to the calculated results.展开更多
Analysis of the mitochondrial proteome would provide valuable insight into the function of this important organelle, which plays key roles in energy metabolism, apoptosis, free radical production, thermogenesis, and c...Analysis of the mitochondrial proteome would provide valuable insight into the function of this important organelle, which plays key roles in energy metabolism, apoptosis, free radical production, thermogenesis, and calcium signaling. It could also increase our understanding about the mechanisms that promote mitochondrial disease. To identify proteins that are antigenically dominant in human liver mitochondria, we generated >240 hybridoma cell lines from native mitochondrial proteins after cell fusion, screening, and cloning. Antibodies that recognized mitochondrial proteins were identified by screening human liver cDNA expression libraries. In this study, we identified 6 major antigens that were recognized by at least 2 different monoclonal antibodies (mAbs). The proteins that were antigenically dominant were: acetyl-Coenzyme A acyltransferase 2 (mitochondrial 3-oxoacyl-Coenzyme A thiolase), aldehyde dehydrogenase 1 family member A1, carbamoyl phosphate synthetase 1, dihydrolipoamide S-acetyltransferase (E2 component of pyruvate dehydrogenase complex), enoyl coenzyme A hydratase 1, and hydroxysteroid (11-beta) dehydrogenase 1. We also determined the subcellular localizations of these enzymes within the mitochondria using immunohistocytochemistry. We believe that these well-characterized antibodies will provide a valuable resource for the Human Liver Proteome Project (HLPP), and will make studies aimed at investigating liver mitochondrial function far easier to perform in future. Our results provide strong evidence that, (i) depletion of dominant proteins from liver mitochondrial samples is possible and, (ii) the approaches adopted in this study can be used to explore or validate protein-protein interactions in this important organelle.展开更多
基金supported by the 10th National Five-Year Program for Science and Technology Development of China(Grant No.2003BA614A-02)
文摘The process of economic activities is on the basis of tremendous material inputs.China has been discharging an enormous amount of waste,giving rise to a wide range of environmental impacts.The method of economy-wide material flow analysis(EW-MFA)is one of the effective tools to examine the flow of materials entering physical economies,and recognize early environmental problems.Relevant researches are still at the early stage in China and most focus on material throughput but are rarely concerned about the utilization of recycling resource and environmental impact.Based on more than 3,000 items of data related,materials entering Chinese economy are classified into three types,and then the characteristics of material input and environmental impact are presented for the years 1990-2005 by using the indicators derived from EW-MFA.The Ratio of Recycled Material(RRM) is added as the new indicator in order to be in accordance with the need of circular economy being promoted in China.Results show that the great changes in the structure of material input cause the continuous increase of industrial solid waste emissions and the bogging down of material productivity.The RRM reveals that the utilization of recycling resources remains at a fairly low level in China.Finally,some weakness of EW-MFA is discussed according to the calculated results.
基金supported by the National Basic Research Program of China (Grant No. 2006CB910803)the National High Technology Research and Development Program of China (Grant No. 2006AA02A311)
文摘Analysis of the mitochondrial proteome would provide valuable insight into the function of this important organelle, which plays key roles in energy metabolism, apoptosis, free radical production, thermogenesis, and calcium signaling. It could also increase our understanding about the mechanisms that promote mitochondrial disease. To identify proteins that are antigenically dominant in human liver mitochondria, we generated >240 hybridoma cell lines from native mitochondrial proteins after cell fusion, screening, and cloning. Antibodies that recognized mitochondrial proteins were identified by screening human liver cDNA expression libraries. In this study, we identified 6 major antigens that were recognized by at least 2 different monoclonal antibodies (mAbs). The proteins that were antigenically dominant were: acetyl-Coenzyme A acyltransferase 2 (mitochondrial 3-oxoacyl-Coenzyme A thiolase), aldehyde dehydrogenase 1 family member A1, carbamoyl phosphate synthetase 1, dihydrolipoamide S-acetyltransferase (E2 component of pyruvate dehydrogenase complex), enoyl coenzyme A hydratase 1, and hydroxysteroid (11-beta) dehydrogenase 1. We also determined the subcellular localizations of these enzymes within the mitochondria using immunohistocytochemistry. We believe that these well-characterized antibodies will provide a valuable resource for the Human Liver Proteome Project (HLPP), and will make studies aimed at investigating liver mitochondrial function far easier to perform in future. Our results provide strong evidence that, (i) depletion of dominant proteins from liver mitochondrial samples is possible and, (ii) the approaches adopted in this study can be used to explore or validate protein-protein interactions in this important organelle.
