BACKGROUND:The non-function and dysfunction of primary liver graft likely involves dependence on Kupffer cells and hepatic innervation. The present experiment was designed to study the expression of P-selectin and int...BACKGROUND:The non-function and dysfunction of primary liver graft likely involves dependence on Kupffer cells and hepatic innervation. The present experiment was designed to study the expression of P-selectin and intercellular adhesion molecule-1(ICAM-1) mRNA in liver graft and to elucidate the role of Kupffer cells and the sympathetic nerve of the liver in down-regulating this expression. METHODS:Donor rats were given hexamethonium,a sympathetic ganglionic blocking agent,and/or gadolinium chloride,an inhibitor of Kupffer cells. Then the changes of graft P-selectin and ICAM-1 mRNA expression were measured after liver transplantation. RESULTS:The expressions of P-selectin and ICAM-1 mRNA were increased after liver transplantation,and down-regulated by liver denervation and elimination of Kupffer cells. CONCLUSIONS:Live donor denervation and elimination of Kupffer cells down-regulated the expressions of P-selectin and ICAM-1 mRNA in grafts. This may decrease graft ischemia/reperfusion injury.展开更多
文摘BACKGROUND:The non-function and dysfunction of primary liver graft likely involves dependence on Kupffer cells and hepatic innervation. The present experiment was designed to study the expression of P-selectin and intercellular adhesion molecule-1(ICAM-1) mRNA in liver graft and to elucidate the role of Kupffer cells and the sympathetic nerve of the liver in down-regulating this expression. METHODS:Donor rats were given hexamethonium,a sympathetic ganglionic blocking agent,and/or gadolinium chloride,an inhibitor of Kupffer cells. Then the changes of graft P-selectin and ICAM-1 mRNA expression were measured after liver transplantation. RESULTS:The expressions of P-selectin and ICAM-1 mRNA were increased after liver transplantation,and down-regulated by liver denervation and elimination of Kupffer cells. CONCLUSIONS:Live donor denervation and elimination of Kupffer cells down-regulated the expressions of P-selectin and ICAM-1 mRNA in grafts. This may decrease graft ischemia/reperfusion injury.
