This study explored the novel strategy of hypoxic preconditioning of Bone Marrow Mesenchymal Stem Cells (BM-MSCs) before intra vitreal transplantation to improve neuroprotective effects of Retinal Ganglion Cells (RGCs...This study explored the novel strategy of hypoxic preconditioning of Bone Marrow Mesenchymal Stem Cells (BM-MSCs) before intra vitreal transplantation to improve neuroprotective effects of Retinal Ganglion Cells (RGCs) in Acute Glaucoma Models. The methods of this research were isolated mesenchymal stem cells from the bone marrow of adult wild-type Sprague-Dawley (SD) rats. BM-MSCs were cultured under normoxic or hypoxic (1% oxygen for 24 hours) conditions. Normoxic or hypoxic BM-MSCs were transplanted intravitreally 1 week after ocular hypertension induction by acutely increasing IOP to 100 - 120 mmHg for 60 minutes. Rats were killed 4 weeks after transplanted. Apoptosis was examined by tunnel assay and expression Brn3b (Brn3b = RGCs marker) by immunohistochemical analysis of the retina. Results showed that transplantation of hypoxic preconditioning BM-MSCs in acute glaucoma models resulted in a significant apoptosis decreasing (p < 0.05) and an significant increasing in RGCs (p < 0.05), as well as enhanced mor-phologic and functional benefits of stem cell therapy versus normoxic BM-MSCs transplantation. Conclusions: Hypoxic preconditioning enhances the capacity of BM-MSCs transplantation to improve neuroprotective effects of RGCs in Acute Glaucoma Models.展开更多
The establishment of the ASEAN Earthquake Information Center (AEIC) was endorsed by the 40th Meeting of the Committee on Science and Technology held in October 2000, at the request of the ASEAN Sub-Committee on Meteor...The establishment of the ASEAN Earthquake Information Center (AEIC) was endorsed by the 40th Meeting of the Committee on Science and Technology held in October 2000, at the request of the ASEAN Sub-Committee on Meteorology and Geophysics (SCMG) to obtain the ASEAN Standing Committee’s (ASC) approval to officially use the name “ASEAN Earthquake Information Center (AEIC)”. As part of AEIC activities, the SCMG implemented a project on the ASEAN Seismic Network for Rapid Exchange of Strong Earthquake Data (ASNET RESED). The project was implemented by the ASCMG with funding support from the Government of Japan through the Japan ASEAN Co-operation Promotion Program (JACPP). The aim of the project is to facilitate the rapid exchange of data and information on earthquakes with magnitudes greater than 5.5 on the Richter scale, and to link all of the ASEAN National Seismological Centers through the Internet to disseminate earthquake information. For the future program, we propose a seismic data exchange between the National Seismological Centers in ASEAN countries as well as world seismological centers using the facilities in the AEIC. The plan is basically to develop and update the software and hardware in the NSC and AEIC for data communication. To refresh our knowledge in seismology and data communication, we arrange the training sessions for both topics.展开更多
Indonesia is situated at the juncture of three major tectonic plates: the Eurasian, the Pacific and the Indo-Australian plates. As a result, many parts of the country are classified as being at a high risk of earthqua...Indonesia is situated at the juncture of three major tectonic plates: the Eurasian, the Pacific and the Indo-Australian plates. As a result, many parts of the country are classified as being at a high risk of earthquake and tsunami disasters. In Indonesia, the Bureau of Meteorology and Geophysics (BMG) is the agency responsible for the monitoring of tectonic earthquakes and tsunamis induced by them. For this purpose, BMG operates seismic networks throughout Indonesia. In order to serve better, BMG will have to improve its monitoring system. Many agencies and universities in Indonesia have done research in the field of seismology, including seismotectonics, earthquake hazards, and so on. Joint research has been done with agencies/universities from abroad. Considering the fact that some earthquake prone areas in Indonesia suffer from frequent disasters, a study on earthquake prediction in some areas of interest would contribute to achieving some long-term goals of the Bureau.展开更多
文摘This study explored the novel strategy of hypoxic preconditioning of Bone Marrow Mesenchymal Stem Cells (BM-MSCs) before intra vitreal transplantation to improve neuroprotective effects of Retinal Ganglion Cells (RGCs) in Acute Glaucoma Models. The methods of this research were isolated mesenchymal stem cells from the bone marrow of adult wild-type Sprague-Dawley (SD) rats. BM-MSCs were cultured under normoxic or hypoxic (1% oxygen for 24 hours) conditions. Normoxic or hypoxic BM-MSCs were transplanted intravitreally 1 week after ocular hypertension induction by acutely increasing IOP to 100 - 120 mmHg for 60 minutes. Rats were killed 4 weeks after transplanted. Apoptosis was examined by tunnel assay and expression Brn3b (Brn3b = RGCs marker) by immunohistochemical analysis of the retina. Results showed that transplantation of hypoxic preconditioning BM-MSCs in acute glaucoma models resulted in a significant apoptosis decreasing (p < 0.05) and an significant increasing in RGCs (p < 0.05), as well as enhanced mor-phologic and functional benefits of stem cell therapy versus normoxic BM-MSCs transplantation. Conclusions: Hypoxic preconditioning enhances the capacity of BM-MSCs transplantation to improve neuroprotective effects of RGCs in Acute Glaucoma Models.
文摘The establishment of the ASEAN Earthquake Information Center (AEIC) was endorsed by the 40th Meeting of the Committee on Science and Technology held in October 2000, at the request of the ASEAN Sub-Committee on Meteorology and Geophysics (SCMG) to obtain the ASEAN Standing Committee’s (ASC) approval to officially use the name “ASEAN Earthquake Information Center (AEIC)”. As part of AEIC activities, the SCMG implemented a project on the ASEAN Seismic Network for Rapid Exchange of Strong Earthquake Data (ASNET RESED). The project was implemented by the ASCMG with funding support from the Government of Japan through the Japan ASEAN Co-operation Promotion Program (JACPP). The aim of the project is to facilitate the rapid exchange of data and information on earthquakes with magnitudes greater than 5.5 on the Richter scale, and to link all of the ASEAN National Seismological Centers through the Internet to disseminate earthquake information. For the future program, we propose a seismic data exchange between the National Seismological Centers in ASEAN countries as well as world seismological centers using the facilities in the AEIC. The plan is basically to develop and update the software and hardware in the NSC and AEIC for data communication. To refresh our knowledge in seismology and data communication, we arrange the training sessions for both topics.
文摘Indonesia is situated at the juncture of three major tectonic plates: the Eurasian, the Pacific and the Indo-Australian plates. As a result, many parts of the country are classified as being at a high risk of earthquake and tsunami disasters. In Indonesia, the Bureau of Meteorology and Geophysics (BMG) is the agency responsible for the monitoring of tectonic earthquakes and tsunamis induced by them. For this purpose, BMG operates seismic networks throughout Indonesia. In order to serve better, BMG will have to improve its monitoring system. Many agencies and universities in Indonesia have done research in the field of seismology, including seismotectonics, earthquake hazards, and so on. Joint research has been done with agencies/universities from abroad. Considering the fact that some earthquake prone areas in Indonesia suffer from frequent disasters, a study on earthquake prediction in some areas of interest would contribute to achieving some long-term goals of the Bureau.
