Evaluation of some biomarkers of inflammation in male Albino Wistar rats following ingestion of Nigerian Bonny Light Crude Oil (NBLCO) and the effect of antioxidant supplementation was the focus of this study. Forty-e...Evaluation of some biomarkers of inflammation in male Albino Wistar rats following ingestion of Nigerian Bonny Light Crude Oil (NBLCO) and the effect of antioxidant supplementation was the focus of this study. Forty-eight adult male Albino Wistar rats weighing 150 - 180 g were randomly divided 8 per group into: group I (control) oral gavaged 3 ml/kg of normal saline;group II received 3 ml/kg of NBLCO;groups III, IV and V received in addition to 3 ml/kg of NBLCO 1 ml/kg vitamin C, vitamin E and 3 ml/kg of honey bee respectively;group VI received 3 ml/kg of honey. After 28 days of treatment, all animals were anaesthetized with chloroform and blood collected by cardiac puncture for analysis. Results showed that NBLCO administration significantly increased erythrocyte sedimentation rate and C-reactive protein while supplementation with antioxidant significantly reversed such effects (p < 0.05). NBLCO significantly reduced platelets and fibrinogen levels compared with the control group (p < 0.05). Interestingly, the vitamin supplementation significantly reduced platelets and fibrinogen levels compared to group II (NBLCO) (p < 0.05). Honey supplementation on the other hand significantly reduced platelets and fibrinogen compared to group II (NBLCO) (p < 0.05). From the results obtained it is concluded that ingestion of NBLCO has the potential to induce development and progression of inflammation but could substantially be mitigated by supplementation with antioxidant vitamins C, E and honey.展开更多
The study investigated the intervention of caffeine on the effect of Nigerian Bonnylight crude oil (NBLCO) on sperm motility and morphology of diabetic Wistar rats. Eighty adult male rats (180 - 200 g body weight) wer...The study investigated the intervention of caffeine on the effect of Nigerian Bonnylight crude oil (NBLCO) on sperm motility and morphology of diabetic Wistar rats. Eighty adult male rats (180 - 200 g body weight) were randomly divided into eight groups of 10 animals each. The control group received 3 mL/Kg body weight of distilled water, ND Caf. group received 20 mL/kg body weight of caffeine, NDCO group received 3 mL/Kg body weight of NBLCO, ND Caf. + CO group received 20 and 3 mL/Kg body weight of caffeine and NBLCO respectively, diabetic group received 3 mL/Kg body weight of distilled water, D Caf. group received 20 mL/Kg body weight of caffeine, D CO received 3 mL/Kg body weight of NBLCO and D Caf. + CO group received 20 and 3 mL/Kg body weight of caffeine and NBLCO respectively, by oral gavaging for 28 days. The results showed that independent administration of caffeine and NBLCO to both non-diabetic and diabetic rats significantly (p < 0.05) altered sperm concentration, morphology and motility. Administration of NBLCO aggravated the worsening condition by significantly (p < 0.05) reducing sperm concentration, motility and increasing abnormal sperms in diabetic rats. Although caffeine significantly (p < 0.05) reduced motility in non-diabetic rats, it did cause any significant alteration in diabetic rats. Diabetes mellitus and NBLCO significantly (p < 0.05) reduced all indices that promote sperm motility while significantly (p < 0.05) increasing indices that do not promote motility. It can be concluded that diabetes mellitus and NBLCO administration have shown the tendency to promote male infertility by reducing sperm motility and adversely altering the morphology of sperm in male Wistar rats, which was ameliorated by caffeine intervention.展开更多
文摘Evaluation of some biomarkers of inflammation in male Albino Wistar rats following ingestion of Nigerian Bonny Light Crude Oil (NBLCO) and the effect of antioxidant supplementation was the focus of this study. Forty-eight adult male Albino Wistar rats weighing 150 - 180 g were randomly divided 8 per group into: group I (control) oral gavaged 3 ml/kg of normal saline;group II received 3 ml/kg of NBLCO;groups III, IV and V received in addition to 3 ml/kg of NBLCO 1 ml/kg vitamin C, vitamin E and 3 ml/kg of honey bee respectively;group VI received 3 ml/kg of honey. After 28 days of treatment, all animals were anaesthetized with chloroform and blood collected by cardiac puncture for analysis. Results showed that NBLCO administration significantly increased erythrocyte sedimentation rate and C-reactive protein while supplementation with antioxidant significantly reversed such effects (p < 0.05). NBLCO significantly reduced platelets and fibrinogen levels compared with the control group (p < 0.05). Interestingly, the vitamin supplementation significantly reduced platelets and fibrinogen levels compared to group II (NBLCO) (p < 0.05). Honey supplementation on the other hand significantly reduced platelets and fibrinogen compared to group II (NBLCO) (p < 0.05). From the results obtained it is concluded that ingestion of NBLCO has the potential to induce development and progression of inflammation but could substantially be mitigated by supplementation with antioxidant vitamins C, E and honey.
文摘The study investigated the intervention of caffeine on the effect of Nigerian Bonnylight crude oil (NBLCO) on sperm motility and morphology of diabetic Wistar rats. Eighty adult male rats (180 - 200 g body weight) were randomly divided into eight groups of 10 animals each. The control group received 3 mL/Kg body weight of distilled water, ND Caf. group received 20 mL/kg body weight of caffeine, NDCO group received 3 mL/Kg body weight of NBLCO, ND Caf. + CO group received 20 and 3 mL/Kg body weight of caffeine and NBLCO respectively, diabetic group received 3 mL/Kg body weight of distilled water, D Caf. group received 20 mL/Kg body weight of caffeine, D CO received 3 mL/Kg body weight of NBLCO and D Caf. + CO group received 20 and 3 mL/Kg body weight of caffeine and NBLCO respectively, by oral gavaging for 28 days. The results showed that independent administration of caffeine and NBLCO to both non-diabetic and diabetic rats significantly (p < 0.05) altered sperm concentration, morphology and motility. Administration of NBLCO aggravated the worsening condition by significantly (p < 0.05) reducing sperm concentration, motility and increasing abnormal sperms in diabetic rats. Although caffeine significantly (p < 0.05) reduced motility in non-diabetic rats, it did cause any significant alteration in diabetic rats. Diabetes mellitus and NBLCO significantly (p < 0.05) reduced all indices that promote sperm motility while significantly (p < 0.05) increasing indices that do not promote motility. It can be concluded that diabetes mellitus and NBLCO administration have shown the tendency to promote male infertility by reducing sperm motility and adversely altering the morphology of sperm in male Wistar rats, which was ameliorated by caffeine intervention.
