Increasing effective panicle number per plant(EPN)is one approach to increase yield potential in rice.However,molecular mechanisms underlying EPN remain unclear.In this study,we integrated mapbased cloning and genome-...Increasing effective panicle number per plant(EPN)is one approach to increase yield potential in rice.However,molecular mechanisms underlying EPN remain unclear.In this study,we integrated mapbased cloning and genome-wide association analysis to identify the EPN4 gene,which is allelic to NARROW LEAF1(NAL1).Overexpression lines containing the Teqing allele(TQ)of EPN4 had significantly increased EPN.NIL-EPN4^(TQ) in japonica(geng)cultivar Lemont(LT)exhibited significantly improved EPN but decreased grain number and flag leaf size relative to LT.Haplotype analysis indicated that accessions with EPN4-1 had medium EPN,medium grain number,and medium grain weight,but had the highest grain yield among seven haplotypes,indicating that EPN4-1 is an elite haplotype of EPN4 for positive coordination of the three components of grain yield.Furthermore,accessions carrying the combination of EPN4-1 and haplotype GNP1-6 of GNP1 for grain number per panicle showed higher grain yield than those with other allele combinations.Therefore,pyramiding of EPN4-1 and GNP1-6 could be a preferred approach to obtain high yield potential in breeding.展开更多
The nutritional quality of rice is a major concern,along with the need to enhance productivity to feed the continuously growing population.Therefore,there is a requirement to breed high-yielding rice varieties with im...The nutritional quality of rice is a major concern,along with the need to enhance productivity to feed the continuously growing population.Therefore,there is a requirement to breed high-yielding rice varieties with improved nutritional quality that can help combat malnutrition,a global health issue.Undoubtedly,breeding approaches have played a significant role in increasing rice yield while enhancing its nutritional content.In addition to traditional breeding techniques,other recent approaches,such as genetic engineering,gene editing,omics methods,and agronomic practices,must also be employed to meet the nutritional needs of the current population.In this review,we offered detailed information on the development of nutritionally improved rice varieties through the enhancement of protein content,microand macronutrients,vitamins,and oil quality using genetic engineering approaches.We also identified QTLs associated with amino acids,proteins,and micronutrients in rice.Furthermore,omics approaches provide a range of tools and techniques for effectively exploring resources and understanding the molecular mechanisms involved in trait development.Omics branches,including transcriptomics,proteomics,ionomics,and metabolomics,are efficiently utilized for improving rice nutrition.Therefore,by utilizing the information obtained from these techniques and incorporating all of these recent approaches,we can effectively modify the rice genome,directly enhancing the nutritional value of rice varieties.This will help address the challenges of malnutrition in the years to come.展开更多
Salinity-alkalinity is incipient abiotic stress that impairs plant growth and development.Rice(Oryza sativa)is a major food crop greatly affected by soil salinity and alkalinity,requiring tolerant varieties in the sal...Salinity-alkalinity is incipient abiotic stress that impairs plant growth and development.Rice(Oryza sativa)is a major food crop greatly affected by soil salinity and alkalinity,requiring tolerant varieties in the saline-alkali prone areas.Understanding the molecular and physiological mechanisms of saline-alkali tolerance paves the base for improving saline-alkali tolerance in rice and leads to progress in breeding.This review illustrated the physiological consequences,and molecular mechanisms especially signaling and function of regulating genes for saline-alkali tolerance in rice plants.We also discussed QTLs regarding saline-alkali tolerance accordingly and ways of deployment for improvement.More efforts are needed to identify and utilize the identified QTLs for saline-alkali tolerance in rice.展开更多
Development of hybrid rice with high yield and grain quality is a goal of rice breeding.To investigate the genetic mechanism of heterosis for rice milling and appearance quality in indica/xian rice,QTL mapping was con...Development of hybrid rice with high yield and grain quality is a goal of rice breeding.To investigate the genetic mechanism of heterosis for rice milling and appearance quality in indica/xian rice,QTL mapping was conducted using 1061 recombinant inbred lines(RILs)derived from a cross of the xian rice cultivars Quan 9311B(Q9311B)and Wu-shan-si-miao(WSSM),and a backcross F_(1)(BC_(1)F_(1)) population developed by crossing the RILs with Quan 9311A(Q9311A),combined with phenotyping in two environments.The F_(1) hybrid(Q9311A×WSSM)showed various degrees of heterosis for milling and appearance quality.A total of 142 main-effect QTL(M-QTL)and 407 pairs of epistatic QTL(E-QTL)were identified for five milling and appearance quality traits and grain yield per plant(GYP)in the RIL,BC_(1)F_(1) and mid-parental heterosis(H_(MP)) populations.Differential detection of QTL in three populations revealed that most additive loci detected in the RILs did not show heterotic effects,but some of them did contribute to BC_(1)F_(1) trait performance.Unlike heterosis of GYP,single-locus overdominance and epistasis were the main contributors to heterosis for milling and appearance quality.Epistasis contributed more to the heterosis for milling quality than to that for appearance quality.Three(four)QTL regions harboring opposite(consistent)directions of favorable allele effects for GYP and grain quality were identified,indicating the presence of partial genetic overlaps between GYP and grain quality.Three strategies are proposed to develop hybrid rice with high yield and good grain quality:1)pyramiding favorable alleles with consistent directions of gene effects for GYP and grain quality at the M-QTL on different chromosomes;2)introgressing favorable alleles for GYP and grain quality into the parents and then pyramiding and fixing these additive effects in hybrids;and 3)pyramiding overdominant and dominant loci and minimizing or eliminating underdominant loci from the parents.展开更多
基金This work was funded by the National Key Research and Development Program of China(2023YFF1000404)the Shenzhen Basic Research and Development Key Program of China(JCYJ20200109150713553)Hainan Key Research and Development in Modern Agriculture of China(ZDYF2021Y128).
文摘Increasing effective panicle number per plant(EPN)is one approach to increase yield potential in rice.However,molecular mechanisms underlying EPN remain unclear.In this study,we integrated mapbased cloning and genome-wide association analysis to identify the EPN4 gene,which is allelic to NARROW LEAF1(NAL1).Overexpression lines containing the Teqing allele(TQ)of EPN4 had significantly increased EPN.NIL-EPN4^(TQ) in japonica(geng)cultivar Lemont(LT)exhibited significantly improved EPN but decreased grain number and flag leaf size relative to LT.Haplotype analysis indicated that accessions with EPN4-1 had medium EPN,medium grain number,and medium grain weight,but had the highest grain yield among seven haplotypes,indicating that EPN4-1 is an elite haplotype of EPN4 for positive coordination of the three components of grain yield.Furthermore,accessions carrying the combination of EPN4-1 and haplotype GNP1-6 of GNP1 for grain number per panicle showed higher grain yield than those with other allele combinations.Therefore,pyramiding of EPN4-1 and GNP1-6 could be a preferred approach to obtain high yield potential in breeding.
基金This study was funded by the Hainan Yazhou Bay Seed Lab Project,China(Grant No.B21HJ0216)the Key Research and Development Project of Hainan Province,China(Grant No.ZDYF2021XDNY128)the Agricultural Science and Technology Innovation Program and the Cooperation and Innovation Mission,China(Grant No.CAAS-ZDXT202001).
文摘The nutritional quality of rice is a major concern,along with the need to enhance productivity to feed the continuously growing population.Therefore,there is a requirement to breed high-yielding rice varieties with improved nutritional quality that can help combat malnutrition,a global health issue.Undoubtedly,breeding approaches have played a significant role in increasing rice yield while enhancing its nutritional content.In addition to traditional breeding techniques,other recent approaches,such as genetic engineering,gene editing,omics methods,and agronomic practices,must also be employed to meet the nutritional needs of the current population.In this review,we offered detailed information on the development of nutritionally improved rice varieties through the enhancement of protein content,microand macronutrients,vitamins,and oil quality using genetic engineering approaches.We also identified QTLs associated with amino acids,proteins,and micronutrients in rice.Furthermore,omics approaches provide a range of tools and techniques for effectively exploring resources and understanding the molecular mechanisms involved in trait development.Omics branches,including transcriptomics,proteomics,ionomics,and metabolomics,are efficiently utilized for improving rice nutrition.Therefore,by utilizing the information obtained from these techniques and incorporating all of these recent approaches,we can effectively modify the rice genome,directly enhancing the nutritional value of rice varieties.This will help address the challenges of malnutrition in the years to come.
基金funded by the Key-Area Research and Development Program of Guangdong Province,China(Grant No.2020B020219004)the Hainan Yazhou Bay Seed Lab(Grant No.B21HJ0216)the Agricultural Science and Technology Innovation Program and the Cooperation and Innovation Mission,China(Grant No.CAAS-ZDXT202001)。
文摘Salinity-alkalinity is incipient abiotic stress that impairs plant growth and development.Rice(Oryza sativa)is a major food crop greatly affected by soil salinity and alkalinity,requiring tolerant varieties in the saline-alkali prone areas.Understanding the molecular and physiological mechanisms of saline-alkali tolerance paves the base for improving saline-alkali tolerance in rice and leads to progress in breeding.This review illustrated the physiological consequences,and molecular mechanisms especially signaling and function of regulating genes for saline-alkali tolerance in rice plants.We also discussed QTLs regarding saline-alkali tolerance accordingly and ways of deployment for improvement.More efforts are needed to identify and utilize the identified QTLs for saline-alkali tolerance in rice.
基金funded by the Key Research and Development Project of Hainan Province(ZDYF2021XDNY128)the Hainan Yazhou Bay Seed Lab Project(B21HJ0216)+1 种基金the Agricultural Science and Technology Innovation Programthe Cooperation and Innovation Mission(CAAS-ZDXT202001)。
文摘Development of hybrid rice with high yield and grain quality is a goal of rice breeding.To investigate the genetic mechanism of heterosis for rice milling and appearance quality in indica/xian rice,QTL mapping was conducted using 1061 recombinant inbred lines(RILs)derived from a cross of the xian rice cultivars Quan 9311B(Q9311B)and Wu-shan-si-miao(WSSM),and a backcross F_(1)(BC_(1)F_(1)) population developed by crossing the RILs with Quan 9311A(Q9311A),combined with phenotyping in two environments.The F_(1) hybrid(Q9311A×WSSM)showed various degrees of heterosis for milling and appearance quality.A total of 142 main-effect QTL(M-QTL)and 407 pairs of epistatic QTL(E-QTL)were identified for five milling and appearance quality traits and grain yield per plant(GYP)in the RIL,BC_(1)F_(1) and mid-parental heterosis(H_(MP)) populations.Differential detection of QTL in three populations revealed that most additive loci detected in the RILs did not show heterotic effects,but some of them did contribute to BC_(1)F_(1) trait performance.Unlike heterosis of GYP,single-locus overdominance and epistasis were the main contributors to heterosis for milling and appearance quality.Epistasis contributed more to the heterosis for milling quality than to that for appearance quality.Three(four)QTL regions harboring opposite(consistent)directions of favorable allele effects for GYP and grain quality were identified,indicating the presence of partial genetic overlaps between GYP and grain quality.Three strategies are proposed to develop hybrid rice with high yield and good grain quality:1)pyramiding favorable alleles with consistent directions of gene effects for GYP and grain quality at the M-QTL on different chromosomes;2)introgressing favorable alleles for GYP and grain quality into the parents and then pyramiding and fixing these additive effects in hybrids;and 3)pyramiding overdominant and dominant loci and minimizing or eliminating underdominant loci from the parents.