Objective:To evaluate in vitro antimicrobial and antioxidant activities of methanolic extract of Jasminum humile（J.humile） leaves extract.Method:Methanolic extract of J.humile was evaluated for its antimicrobial act...Objective:To evaluate in vitro antimicrobial and antioxidant activities of methanolic extract of Jasminum humile（J.humile） leaves extract.Method:Methanolic extract of J.humile was evaluated for its antimicrobial activity by using agar well diffusion method & their possible antioxidant assay by two complementary test systems,namely DPPH and hydrogen peroxide scavenging activity.These various antioxidant activities were compared to standard antioxidants such as ascorbic acid for both the tests.Results:In the DPPH & hydrogen peroxide scavenging activity,the IC<sub>50</sub> value of methanol extract was 70.43μg/mL & 60.79μg/mL respectively. Further,the extract showed inhibitory activity for Gram-positive and negative bacteria at different concentrations.The maximum antibacterial activity of extract was exhibited against Staphylococcus aureus（S.aureus） at concentration 50 mg/mL when compared with ciprofloxacin Conclusions:These results clearly indicate that J.humile is effective in scavenging free radicals and has the potential to be a powerful antioxidant.Thus,the results obtained in the present study indicate that J.humile leaves extract could be considered as a potential source of natural antioxidants and that could be used as an effective source against bacterial diseases.展开更多
Short interfering RNA (siRNA) is a powerful technique that can suppress gene expression in a variety of cells including mammalian cells. Endothelial progenitor cells (EPCs) are bone marrow—derived haematopoietic prog...Short interfering RNA (siRNA) is a powerful technique that can suppress gene expression in a variety of cells including mammalian cells. Endothelial progenitor cells (EPCs) are bone marrow—derived haematopoietic progenitor cells that have been implicated in vasculogenesis. We demonstrated for the first time that gene silencing of endothelial E-selectin using siRNA transfection in human umbilical vein endothelial cells (HUVECs) causes inhibition of EPC adhesion under flow conditions. Fluorescence immunobinding assay analysis showed that significant reduction of E-selectin surface expression in HUVECs (activated with IL-1β (10 U/mL) for 4 h) transfected with siRNA against E-selectin, but not in HUVECs transfected with LacZ siRNA (control). An EPC adhesion assay under flow conditions (shear stress = 1.0 dyne/cm2) then demonstrated that HUVECs transfected with E-selectin siRNA supported significantly less adhesion of EPCs than those HUVECs treated with control siRNA and no siRNA after activation by IL-1β (p 【0.05). Our experiments have shown the importance of E-selectin in EPC adhesion to HUVECs and the potential utility of gene silencing of E-selectin in EPC recruitment.展开更多
文摘Objective:To evaluate in vitro antimicrobial and antioxidant activities of methanolic extract of Jasminum humile（J.humile） leaves extract.Method:Methanolic extract of J.humile was evaluated for its antimicrobial activity by using agar well diffusion method & their possible antioxidant assay by two complementary test systems,namely DPPH and hydrogen peroxide scavenging activity.These various antioxidant activities were compared to standard antioxidants such as ascorbic acid for both the tests.Results:In the DPPH & hydrogen peroxide scavenging activity,the IC<sub>50</sub> value of methanol extract was 70.43μg/mL & 60.79μg/mL respectively. Further,the extract showed inhibitory activity for Gram-positive and negative bacteria at different concentrations.The maximum antibacterial activity of extract was exhibited against Staphylococcus aureus（S.aureus） at concentration 50 mg/mL when compared with ciprofloxacin Conclusions:These results clearly indicate that J.humile is effective in scavenging free radicals and has the potential to be a powerful antioxidant.Thus,the results obtained in the present study indicate that J.humile leaves extract could be considered as a potential source of natural antioxidants and that could be used as an effective source against bacterial diseases.
文摘Short interfering RNA (siRNA) is a powerful technique that can suppress gene expression in a variety of cells including mammalian cells. Endothelial progenitor cells (EPCs) are bone marrow—derived haematopoietic progenitor cells that have been implicated in vasculogenesis. We demonstrated for the first time that gene silencing of endothelial E-selectin using siRNA transfection in human umbilical vein endothelial cells (HUVECs) causes inhibition of EPC adhesion under flow conditions. Fluorescence immunobinding assay analysis showed that significant reduction of E-selectin surface expression in HUVECs (activated with IL-1β (10 U/mL) for 4 h) transfected with siRNA against E-selectin, but not in HUVECs transfected with LacZ siRNA (control). An EPC adhesion assay under flow conditions (shear stress = 1.0 dyne/cm2) then demonstrated that HUVECs transfected with E-selectin siRNA supported significantly less adhesion of EPCs than those HUVECs treated with control siRNA and no siRNA after activation by IL-1β (p 【0.05). Our experiments have shown the importance of E-selectin in EPC adhesion to HUVECs and the potential utility of gene silencing of E-selectin in EPC recruitment.
