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非小细胞肺癌中lncRNA DLEU1的表达对肿瘤细胞转移的影响 被引量:5
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作者 张杰 刘索 +1 位作者 甘毅 金龙玉 《中国肿瘤临床》 CAS CSCD 北大核心 2018年第23期1181-1186,共6页
目的:研究长片段非编码RNA(long non-coding RNA,lncRNA)淋巴细胞白血病缺失基因1(deleted in lymphocytic leukemia1,DLEU1)在非小细胞肺癌(non-small cell lung cancer,NSCLC)中的表达模式和临床意义,并进一步评估其对肿瘤转移的影响... 目的:研究长片段非编码RNA(long non-coding RNA,lncRNA)淋巴细胞白血病缺失基因1(deleted in lymphocytic leukemia1,DLEU1)在非小细胞肺癌(non-small cell lung cancer,NSCLC)中的表达模式和临床意义,并进一步评估其对肿瘤转移的影响。方法:42例NSCLC患者配对的癌组织和癌旁组织于2008年1月至2012年12月在中南大学湘雅三医院经手术切除获得。采用实时定量聚合酶链反应检测NSCLC组织和细胞中DLEU1的表达特征。运用统计学方法分析DLEU1的表达与NSCLC患者临床病理特征及生存时间的关系。通过体外伤口愈合和细胞侵袭试验分析DLEU1对NSCLC细胞迁移及侵袭的影响。运用蛋白质印迹法检测DLEU1对NSCLC细胞上皮间质转化(EMT)的标志物(E-钙粘蛋白、N-钙黏蛋白和波形蛋白)表达量的影响。结果:42例NSCLC样本中,DLEU1的表达在35例中表现出上调(83.33%),而在7例中表现出下调(16.67%)。DLEU1在NSCLC癌组织的表达量是癌旁组织的2.11倍(P<0.05)。DLEU1在4种NSCLC细胞系(A549、H1299、SPCA1和H358)的表达量均高于正常肺16HBE上皮细胞,其中,A549细胞中DLEU1表达量最高(P<0.05)。高表达的DLEU1与淋巴结转移呈正相关(P<0.05),但与其他参数无显著相关性,包括患者性别、年龄、吸烟史、原发肿瘤大小、组织学分级和TNM分期。与低DLEU1表达组患者相比,高表达组患者生存时间显著缩短(P<0.05)。与对照组相比,体外干扰DLEU1的表达可显著抑制A549和SPCA1细胞的迁移和侵袭能力(P<0.05)。与对照组相比,体外干扰DLEU1可显著增加A549细胞中E-钙黏蛋白表达量,而降低N-钙黏蛋白和波形蛋白表达量(P<0.05)。结论:DLEU1在NSCLC组织和细胞系中表达上调,并与患者淋巴结转移和生存时间相关,且具有通过EMT促进肿瘤细胞迁移和侵袭的功能,表明DLEU1可能作为NSCLC的潜在治疗靶点。 展开更多
关键词 淋巴细胞白血病缺失基因1 非小细胞肺癌 上调 细胞 转移
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聚甲基丙烯酸甲酯、聚氨酯和氧化锌复合纳米纤维膜的制备及其光催化性能 被引量:1
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作者 刘锁 凤权 +2 位作者 胡金燕 武丁胜 李曼 《环境化学》 CAS CSCD 北大核心 2021年第5期1567-1574,共8页
利用静电纺丝制备具有良好纤维形态及优异力学性能的聚甲基丙烯酸甲酯、聚氨酯和氧化锌(PMMA/PU/ZnO)复合纳米纤维膜,采用等离子技术对复合纳米纤维膜进行亲水性修饰,并探究其对染料废水的光催化降解性能。本文对PMMA/PU/ZnO复合纳米纤... 利用静电纺丝制备具有良好纤维形态及优异力学性能的聚甲基丙烯酸甲酯、聚氨酯和氧化锌(PMMA/PU/ZnO)复合纳米纤维膜,采用等离子技术对复合纳米纤维膜进行亲水性修饰,并探究其对染料废水的光催化降解性能。本文对PMMA/PU/ZnO复合纳米纤维膜的微观形态结构进行详细表征,并对其亲水性能、力学性能及重复使用性能予以测试。结果表明,在300 W汞灯照射条件下,经等离子处理的PMMA/PU/ZnO复合纳米纤维膜对亚甲基蓝、罗丹明B和活性红的降解率分别为85%、59%和52%,经3次重复使用后仍具有良好的降解性能。 展开更多
关键词 聚甲基丙烯酸甲酯 等离子处理 纳米氧化锌 光催化
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Data reduction and calibration of the FMG onboard ASO-S 被引量:8
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作者 Jiang-Tao Su Xian-Yong Bai +12 位作者 Jie Chen Jing-Jing Guo suo liu Xiao-Fan Wang Hai-Qing Xu Xiao Yang Yong-Liang Song Yuan-Yong Deng Kai-Fan Ji Lei Deng Yu Huang Hui Li Wei-Qun Gan 《Research in Astronomy and Astrophysics》 SCIE CAS CSCD 2019年第11期63-78,共16页
The Full-disk vector Magneto Graph(FMG)instrument will carry out polarization observations at one wavelength position of the Fe I 5324.179?spectral line.This paper describes how to choose this single wavelength positi... The Full-disk vector Magneto Graph(FMG)instrument will carry out polarization observations at one wavelength position of the Fe I 5324.179?spectral line.This paper describes how to choose this single wavelength position,the relevant data-processing and the magnetic field calibrations based on the measured polarization signals at one single wavelength position.It is found that solar radial Doppler velocity,which can cause the spectral line to shift,is a disadvantageous factor for the linear calibration at one wavelength position.Observations at two symmetric wavelength positions may significantly reduce the wavelength shift effect(~75%),but simulations show that such polarization signals located at the solar limbs(e.g.,beyond the longitude range of±30°)are not free from the effect completely.In future work,we plan to apply machine learning techniques to calibrate vector magnetic fields,or employ full Stokes parameter profile inversion techniques to obtain accurate vector magnetic fields,in order to complement the linear calibration at the single wavelength position. 展开更多
关键词 SUN photosphere-Sun MAGNETIC fields-Sun CALIBRATION
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Superpenumbral chromospheric flare 被引量:1
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作者 suo liu Hong-Qi Zhang +2 位作者 Debiprasad P.Choudhary Abhishek K.Srivastava Bhola Nath Dwivedi 《Research in Astronomy and Astrophysics》 SCIE CAS CSCD 2018年第10期131-140,共10页
We observed a C-class flare at the outer boundary of the superpenumbra of a sunspot. The flare was triggered by an emerging magnetic bipolar region that was obliquely oriented with respect to the superpenumbral fibril... We observed a C-class flare at the outer boundary of the superpenumbra of a sunspot. The flare was triggered by an emerging magnetic bipolar region that was obliquely oriented with respect to the superpenumbral fibrils. The flare started due to the low height magnetic reconnection of emerging magnetic flux with a superpenumbral field resulting in hot multi-temperature plasma flows in the inverse Evershed flow channel and its overlying atmosphere. Inverse Evershed flows in the chromosphere start from the superpenumbra towards the sunspot that end at the outer boundary of the penumbra. The hot plasma flow towards the sunspot in the inverse Evershed channels show about 10 km s^-1 higher velocity in Ha wavelengths compared to the plasma emissions at various temperatures as seen in different AIA filters. Even though these velocities are about seven times higher than the typical inverse Evershed flow speeds, the flow is diminished at the outer boundary of the sunspot's penumbra. This suggests that the superpenumbral field lines that carry inverse Evershed flows are discontinued at the boundary where the penumbral field lines dive into the sun and these two sets of field lines are completely distinct. The discontinuity in the typical magnetic field and plasma properties at the adjoining of these two sets of field lines further leads to discontinuity in the characteristic magnetoacoustic and Alfvrn speeds, thereby stopping the plasma flows further on. The multi-temperature plasma in the inverse Evershed channels exhibits possible longitudinal oscillations initially during the onset of the flare, and later flows towards the sunspot. In the multi-temperature view, the different layers above the flare region have a mixture of supersonic as well as subsonic flows. 展开更多
关键词 magnetic field solar flare TRIGGER magnetic emergence
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Automatic detection and correction algorithms for magnetic saturation in the SMFT/HSOS longitudinal magnetograms
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作者 Hai-Qing Xu suo liu +8 位作者 Jiang-Tao Su Yuan-Yong Deng Andrei Plotnikov Xian-Yong Bai Jie Chen Xiao Yang Jing-Jing Guo Xiao-Fan Wang Yong-Liang Song 《Research in Astronomy and Astrophysics》 SCIE CAS CSCD 2021年第3期158-166,共9页
A longitudinal magnetic field often suffers the saturation effect in a strong magnetic field region when the measurement is performed at a single-wavelength point and linear calibration is adopted.In this study,we dev... A longitudinal magnetic field often suffers the saturation effect in a strong magnetic field region when the measurement is performed at a single-wavelength point and linear calibration is adopted.In this study,we develop a method that can judge the threshold of saturation in Stokes V/I observed by the Solar Magnetic Field Telescope(SMFT)and correct it automatically.The procedure is to first perform the second-order polynomial fit to the Stokes V/I vs.I/I_(m)(I_(m) is the maximum value of Stokes I)curve to estimate the threshold of saturation,then reconstruct Stokes V/I in a strong field region to correct for saturation.The algorithm is demonstrated to be effective by comparing with the magnetograms obtained by the Helioseismic and Magnetic Imager(HMI).The accuracy rate of detection and correction for saturation is~99.4%and~88%respectively among 175 active regions.The advantages and disadvantages of the algorithm are discussed. 展开更多
关键词 Sun:sunspots Sun:magnetic field Methods:data analysis
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Antibiotic resistome mostly relates to bacterial taxonomy along a suburban transmission chain 被引量:1
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作者 Ziyan Qin Qun Gao +8 位作者 Qiang Dong Joy D.Van Nostrand Qi Qi Yifan Su suo liu Tianjiao Dai Jingmin Cheng Jizhong Zhou Yunfeng Yang 《Frontiers of Environmental Science & Engineering》 SCIE EI CSCD 2022年第3期63-72,共10页
Antibiotic resistance genes comprising antibiotic resistome are of great concern due to their increase in the environment.Recent evidence of shared resistomes between soils and animal husbandry has imposed potential r... Antibiotic resistance genes comprising antibiotic resistome are of great concern due to their increase in the environment.Recent evidence of shared resistomes between soils and animal husbandry has imposed potential risks to human health.However,the correlation between a given community’s resistome and bacterial taxonomic composition is controversial.Here,a transmission chain of resistomes from swine manure to compost and compost-amended soil were analyzed in five suburban areas of Beijing,China,with unamended agricultural soils as control soils.Antibiotic resistomes and bacterial taxonomic compositions were distinct between(I)manure and compost;and(II)compost-amended and control soils.In manure,compost,and compost-amended soils,theβ-diversity of the resistome and bacterial taxonomic composition was significantly correlated,while no correlation was detected in control soils.Bacterial taxonomic composition explained 36.0%of total variations of the resistome composition,much higher than environmental factors.Together,those results demonstrated that antibiotic resistome was closely related to bacterial taxonomic composition along the suburban transmission chain. 展开更多
关键词 Antibiotic resistance genes RESISTOME Bacterial taxonomy Transmission chain
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