AaMYB3 interacts with AabHLH1 to regulate proanthocyanidin accumulation in Anthurium andraeanum(Hort.)—another strategy to modulate pigmentation

Proanthocyanidins(PAs),also known as“condensed tannins”,are colorless metabolites produced through the flavonoid pathway that are involved in stress resistance in plants.Because PAs are involved in the anthocyanin biosynthetic pathway,they play a role in the modification of pigmentation conferred by anthocyanins in ornamental organs.In this study,we isolated the gene and functionally characterized an R2R3-MYB transcription factor(TF),AaMYB3,and a basic helix-loop-helix TF,AabHLH1,from Anthurium andraeanum(Hort.),a typical tropical flower.AaMYB3 is primarily expressed in the spathe and negatively correlates with anthocyanin accumulation.A complementation test in an Arabidopsis tt8 mutant showed that AabHLH1 successfully restores the PA-deficient seed coat phenotype.The ectopic overexpression of AaMYB3 alone or its coexpression with AabHLH1 in transgenic tobacco resulted in light pink or even pale-pink corolla limbs by reducing their anthocyanin levels and greatly enhancing their accumulation of PAs.This overexpression of the anthurium TF genes upregulated the late anthocyanin enzymeencoding genes(NtDFR and NtANS)and the key PA genes(NtLAR and NtANR)in transgenic tobacco.The interaction between AaMYB3 and the AabHLH1 protein was confirmed using yeast two-hybrid(Y2H)and bimolecular fluorescence complementation(BiFC)assays.In the developing red spathes of the cultivars“Vitara”and“Tropical”,the expression of AaMYB3 was closely linked to PA accumulation,and AaMYB3 was coexpressed with AaCHS,AaF3H,AaDFR,AaANS,AaLAR,and AaANR.The expression pattern of AabHLH1 was similar to that of AaF3′H.Our results suggest that AaMYB3 and AabHLH1 are involved in the regulation of PA biosynthesis in anthurium and could potentially be used to metabolically engineer PA biosynthesis in plants.

Integration of Peridynamic Theory and OpenSees for Solving Problems in Civil Engineering

Peridynamics(PD)is a powerful method to simulate the discontinuous problems in civil engineering.However,it may take a lot of effort to implement the material constitutive models into PD program for solving a broad range of problems.OpenSees is an open source software which includes a versatile material library and has been widely used by researchers and engineers in civil engineering.In this context,the paper presents a simple but effective approach to integrate PD with OpenSees by using a Client-Server(CS)software integration technique,such that the existing material constitutive models in OpenSees can be directly used by PD.Two applications are presented to verify the new PD-OpenSees platform.The first one is a plate with a pre-crack subject to horizontal load simulated using a three-dimension(3D)multi-yield-surfaces plasticity model,and the second one is a concrete block with a rectangular hole subject to a uniaxial loading condition simulated using a 3D Cap plasticity model.It shows that the generation/propagation of cracks of the elastoplastic materials(e.g.,concrete and soil)can be analyzed by combing PD and three-dimensional plasticity constitutive models without compiling/linking these complex material models into PD program.Thus,the integrated PDOpenSees platform presented herein is potentially capable to solve a wide range of complex problems in civil engineering.

An High-Speed Low-Noise Rotor Topology for EV/HEV PMSM

Permanent magnet synchronous motor(PMSM)for EV/HEV pursues higher efficiency,higher power density,higher rotation speed and better NVH performance.To meet these requirements,an improved triangle rotor topology is presented.The new rotor topology is researched through comparing the performance with V shape rotor and traditional triangle shape rotor.The comparing results on air-gap flux density,key order radial forces,sound pressure,inductance,torque,anti-demagnetization capability and efficiency proved the advantage of the new rotor topology.