AIM: To develop a sensitive method for measuring theputative endocannabinoid 2-arachidonylglycerol (2-AG)in the peripheral and ceatral nervous system.METHODS: A method using atmospheric pressurechemical ionization (AP...AIM: To develop a sensitive method for measuring theputative endocannabinoid 2-arachidonylglycerol (2-AG)in the peripheral and ceatral nervous system.METHODS: A method using atmospheric pressurechemical ionization (APCI) liquid chromatography/mass spectrometry (LC/MS ) was developed todetermine the levels of 2-AG in methanol extracts of therat lumar spinal cord, dorsal root ganglion (DRG),and sciatic nerve. RESULTS: 2-AG was detectedwith high sensitivity and minimal sample preparation.The levels in the tissues analyzed were ≤pmol/mg wetweight. Similar levels were found in the spinal展开更多
文摘AIM: To develop a sensitive method for measuring theputative endocannabinoid 2-arachidonylglycerol (2-AG)in the peripheral and ceatral nervous system.METHODS: A method using atmospheric pressurechemical ionization (APCI) liquid chromatography/mass spectrometry (LC/MS ) was developed todetermine the levels of 2-AG in methanol extracts of therat lumar spinal cord, dorsal root ganglion (DRG),and sciatic nerve. RESULTS: 2-AG was detectedwith high sensitivity and minimal sample preparation.The levels in the tissues analyzed were ≤pmol/mg wetweight. Similar levels were found in the spinal
