目的:观察针刺推拿结合治疗Sudeck骨萎缩的临床疗效。方法:将92例Sudeck骨萎缩患者随机分为治疗组和对照组各46例,治疗组采用针刺加推拿手法,对照组采用单纯针刺法,以临床疗效、评定疼痛的视觉模拟评分(visual analogue scale VAS)及腕...目的:观察针刺推拿结合治疗Sudeck骨萎缩的临床疗效。方法:将92例Sudeck骨萎缩患者随机分为治疗组和对照组各46例,治疗组采用针刺加推拿手法,对照组采用单纯针刺法,以临床疗效、评定疼痛的视觉模拟评分(visual analogue scale VAS)及腕关节功能Dienst分级评分为观察指标。结果:两组均取得明显的疗效,且治疗组明显优于对照组(P<0.01);与对照组相比,VAS评分(cm,2.01±2.03 vs 3.16±2.22)及Dienst评分(优21 vs 11例;良15 vs 12例;可6 vs 14例;差4 vs 9例)均有显著性差异(P<0.01)。结论:针刺、推拿疗法治疗Sudeck骨萎缩疗效确切,针刺推拿结合法的疗效明显优于单纯针刺法,尤其在消肿止痛、改善腕关节功能方面具有显著的优势,值得推广应用。展开更多
There is increasing evidence that pathogens do not only elicit direct defense responses, but also cause pronounced changes in primary carbohydrate metabolism. Cell-wall-bound invertases belong to the key regulators of...There is increasing evidence that pathogens do not only elicit direct defense responses, but also cause pronounced changes in primary carbohydrate metabolism. Cell-wall-bound invertases belong to the key regulators of carbohydrate partitioning and source-sink relations. Whereas studies have focused so far only on the transcriptional induction of invertase genes in response to pathogen infection, the role of post-translational regulation of invertase activity has been neglected and was the focus of the present study. Expression analyses revealed that the high mRNA level of one out of three proteinaceous invertase inhibitors in source leaves of Arabidopsis thaliana is strongly repressed upon infection by a virulent strain of Pseudomonas syringae pv. tomato DC3000. This repression is paralleled by a decrease in invertase inhibitor activity. The physiological role of this regulatory mechanism is revealed by the finding that in situ invertase activity was detectable only upon infection by P. syringae. In contrast, a high invertase activity could be measured in vitro in crude and cell wall extracts prepared from both infected and non-infected leaves. The discrepancy between the in situ and in vitro invertase activity of control leaves and the high in situ invertase activity in infected leaves can be explained by the pathogen-dependent repression of invertase inhibitor expression and a concomitant reduction in invertase inhibitor activity. The functional importance of the release of invertase from post-translational inhibition for the defense response was substantiated by the application of the competitive chemical invertase inhibitor acarbose. Posttranslational inhibition of extracellular invertase activity by infiltration of acarbose in leaves was shown to increase the susceptibility to P. syringae. The impact of invertase inhibition on spatial and temporal dynamics of the repression of photosynthesis and promotion of bacterial growth during pathogen infection supports a role for extracellular invertase in plant defense. The acarbose-mediated increase in susceptibility was also detectable in sid2 and cpr6 mutants and resulted in slightly elevated levels of salicylic acid, demonstrating that the effect is independent of the salicylic acid-regulated defense pathway. These findings provide an explanation for high extractable invertase activity found in source leaves that is kept inhibited in situ by post-translational interaction between invertase and the invertase inhibitor proteins. Upon pathogen infection, the invertase activity is released by repression of invertase inhibitor expression, thus linking the local induction of sink strength to the plant defense response.展开更多
文摘目的:观察针刺推拿结合治疗Sudeck骨萎缩的临床疗效。方法:将92例Sudeck骨萎缩患者随机分为治疗组和对照组各46例,治疗组采用针刺加推拿手法,对照组采用单纯针刺法,以临床疗效、评定疼痛的视觉模拟评分(visual analogue scale VAS)及腕关节功能Dienst分级评分为观察指标。结果:两组均取得明显的疗效,且治疗组明显优于对照组(P<0.01);与对照组相比,VAS评分(cm,2.01±2.03 vs 3.16±2.22)及Dienst评分(优21 vs 11例;良15 vs 12例;可6 vs 14例;差4 vs 9例)均有显著性差异(P<0.01)。结论:针刺、推拿疗法治疗Sudeck骨萎缩疗效确切,针刺推拿结合法的疗效明显优于单纯针刺法,尤其在消肿止痛、改善腕关节功能方面具有显著的优势,值得推广应用。
文摘There is increasing evidence that pathogens do not only elicit direct defense responses, but also cause pronounced changes in primary carbohydrate metabolism. Cell-wall-bound invertases belong to the key regulators of carbohydrate partitioning and source-sink relations. Whereas studies have focused so far only on the transcriptional induction of invertase genes in response to pathogen infection, the role of post-translational regulation of invertase activity has been neglected and was the focus of the present study. Expression analyses revealed that the high mRNA level of one out of three proteinaceous invertase inhibitors in source leaves of Arabidopsis thaliana is strongly repressed upon infection by a virulent strain of Pseudomonas syringae pv. tomato DC3000. This repression is paralleled by a decrease in invertase inhibitor activity. The physiological role of this regulatory mechanism is revealed by the finding that in situ invertase activity was detectable only upon infection by P. syringae. In contrast, a high invertase activity could be measured in vitro in crude and cell wall extracts prepared from both infected and non-infected leaves. The discrepancy between the in situ and in vitro invertase activity of control leaves and the high in situ invertase activity in infected leaves can be explained by the pathogen-dependent repression of invertase inhibitor expression and a concomitant reduction in invertase inhibitor activity. The functional importance of the release of invertase from post-translational inhibition for the defense response was substantiated by the application of the competitive chemical invertase inhibitor acarbose. Posttranslational inhibition of extracellular invertase activity by infiltration of acarbose in leaves was shown to increase the susceptibility to P. syringae. The impact of invertase inhibition on spatial and temporal dynamics of the repression of photosynthesis and promotion of bacterial growth during pathogen infection supports a role for extracellular invertase in plant defense. The acarbose-mediated increase in susceptibility was also detectable in sid2 and cpr6 mutants and resulted in slightly elevated levels of salicylic acid, demonstrating that the effect is independent of the salicylic acid-regulated defense pathway. These findings provide an explanation for high extractable invertase activity found in source leaves that is kept inhibited in situ by post-translational interaction between invertase and the invertase inhibitor proteins. Upon pathogen infection, the invertase activity is released by repression of invertase inhibitor expression, thus linking the local induction of sink strength to the plant defense response.
