Seedling selection identifies superior seedlings as candidate cultivars based on predicted genetic potential for traits of interest.Traditionally,genetic potential is determined by phenotypic evaluation.With the avail...Seedling selection identifies superior seedlings as candidate cultivars based on predicted genetic potential for traits of interest.Traditionally,genetic potential is determined by phenotypic evaluation.With the availability of DNA tests for some agronomically important traits,breeders have the opportunity to include DNA information in their seedling selection operations—known as marker-assisted seedling selection.A major challenge in deploying marker-assisted seedling selection in clonally propagated crops is a lack of knowledge in genetic gain achievable from alternative strategies.Existing models based on additive effects considering seed-propagated crops are not directly relevant for seedling selection of clonally propagated crops,as clonal propagation captures all genetic effects,not just additive.This study modeled genetic gain from traditional and various marker-based seedling selection strategies on a single trait basis through analytical derivation and stochastic simulation,based on a generalized seedling selection scheme of clonally propagated crops.Various trait-test scenarios with a range of broad-sense heritability and proportion of genotypic variance explained by DNA markers were simulated for two populations with different segregation patterns.Both derived and simulated results indicated that marker-based strategies tended to achieve higher genetic gain than phenotypic seedling selection for a trait where the proportion of genotypic variance explained by marker information was greater than the broad-sense heritability.Results from this study provides guidance in optimizing genetic gain from seedling selection for single traits where DNA tests providing marker information are available.展开更多
基金This work was funded by USDA’s National Institute of Food and Agriculture-Specialty Crop Research Initiative project,‘RosBREED:Enabling Marker-Assisted Breeding in Rosaceae’(2009-51181-05808)‘Tree Fruit GDR:Translating Genomics into Advances in Horticulture’(2009-51181-06036)+2 种基金‘RosBREED:Combining Disease Resistance and Horticultural Quality in New Rosaceous Cultivars’(2014-51181-22378)‘GDR:Empowering Specialty Crop Research through Big-Data Driven Discovery and Application in Breeding’(2014-51181-223760)USDA Hatch funds provided to the Department of Horticulture,Washington State University.
文摘Seedling selection identifies superior seedlings as candidate cultivars based on predicted genetic potential for traits of interest.Traditionally,genetic potential is determined by phenotypic evaluation.With the availability of DNA tests for some agronomically important traits,breeders have the opportunity to include DNA information in their seedling selection operations—known as marker-assisted seedling selection.A major challenge in deploying marker-assisted seedling selection in clonally propagated crops is a lack of knowledge in genetic gain achievable from alternative strategies.Existing models based on additive effects considering seed-propagated crops are not directly relevant for seedling selection of clonally propagated crops,as clonal propagation captures all genetic effects,not just additive.This study modeled genetic gain from traditional and various marker-based seedling selection strategies on a single trait basis through analytical derivation and stochastic simulation,based on a generalized seedling selection scheme of clonally propagated crops.Various trait-test scenarios with a range of broad-sense heritability and proportion of genotypic variance explained by DNA markers were simulated for two populations with different segregation patterns.Both derived and simulated results indicated that marker-based strategies tended to achieve higher genetic gain than phenotypic seedling selection for a trait where the proportion of genotypic variance explained by marker information was greater than the broad-sense heritability.Results from this study provides guidance in optimizing genetic gain from seedling selection for single traits where DNA tests providing marker information are available.
