Objective:To analyze the phytochemicals in twelve species of marine phytoplankton.Methods:Total phenolic content of methanol extract was estimated by the Folin-Ciocalteu method.Total flavonoid content of the methanol ...Objective:To analyze the phytochemicals in twelve species of marine phytoplankton.Methods:Total phenolic content of methanol extract was estimated by the Folin-Ciocalteu method.Total flavonoid content of the methanol extarct was determined by aluminium chloride method.Chlorophylls,β-carotene and astaxanthin were estimated by acetone extraction method.Vitamin C was determined by dinitrophenyl-hydrazine method.Phycobiliproteins such as allophycocyanin,phycocyanin and phycoerythrin in the aqueous extracts were determined.Results:Total phenolics varied from 5.41 mg gallic acid equivalents/g dry weight(DW)in Phormidium corium(P.corium)to 17.37 mg gallic acid equivalents/g DW in Oscillatoria fremyii(O.fremyii).Total flavonoids ranged between 0.74 mg quercetin equivalent/g DW in P.corium and 9.87 mg quercetin equivalent/g DW in Nannochloropsis oceanica.Chlorophyll-a pigment was high in Chaetoceros calcitrans(C.calcitrans)(15.51 mg/g DW)and low in P.corium(1.08 mg/g DW).Chlorophyll-c ranged between 0.07 mg/g DW in Nannochloropsis oceanica and 4.62 mg/g DW in C.calcitrans.High contents ofβ-carotene and astaxanthin were found in C.calcitrans and low in P.corium which ranged from 0.33 to 10.03 mg/g DW and 0.18 to 3.85 mg/g DW,respectively.Vitamin C content varied from 0.50 mg/g DW in C.calcitrans to 1.51 mg/g DW in Phormidium tenue.O.fremyii showed highest total phycobiliproteins of 317.05 mg/g DW.High contents of allophycocyanin and phycocyanin were found in O.fremyii,whereas high contents of phycoerythrin were found in Oscillatoria sancta.All the three phycobiliproteins were low in Chroococcus turgidus.Conclusions:Marine phytoplankton are one of the natural sources providing novel biologically active compounds with potential for pharmaceutical applications.展开更多
文摘Objective:To analyze the phytochemicals in twelve species of marine phytoplankton.Methods:Total phenolic content of methanol extract was estimated by the Folin-Ciocalteu method.Total flavonoid content of the methanol extarct was determined by aluminium chloride method.Chlorophylls,β-carotene and astaxanthin were estimated by acetone extraction method.Vitamin C was determined by dinitrophenyl-hydrazine method.Phycobiliproteins such as allophycocyanin,phycocyanin and phycoerythrin in the aqueous extracts were determined.Results:Total phenolics varied from 5.41 mg gallic acid equivalents/g dry weight(DW)in Phormidium corium(P.corium)to 17.37 mg gallic acid equivalents/g DW in Oscillatoria fremyii(O.fremyii).Total flavonoids ranged between 0.74 mg quercetin equivalent/g DW in P.corium and 9.87 mg quercetin equivalent/g DW in Nannochloropsis oceanica.Chlorophyll-a pigment was high in Chaetoceros calcitrans(C.calcitrans)(15.51 mg/g DW)and low in P.corium(1.08 mg/g DW).Chlorophyll-c ranged between 0.07 mg/g DW in Nannochloropsis oceanica and 4.62 mg/g DW in C.calcitrans.High contents ofβ-carotene and astaxanthin were found in C.calcitrans and low in P.corium which ranged from 0.33 to 10.03 mg/g DW and 0.18 to 3.85 mg/g DW,respectively.Vitamin C content varied from 0.50 mg/g DW in C.calcitrans to 1.51 mg/g DW in Phormidium tenue.O.fremyii showed highest total phycobiliproteins of 317.05 mg/g DW.High contents of allophycocyanin and phycocyanin were found in O.fremyii,whereas high contents of phycoerythrin were found in Oscillatoria sancta.All the three phycobiliproteins were low in Chroococcus turgidus.Conclusions:Marine phytoplankton are one of the natural sources providing novel biologically active compounds with potential for pharmaceutical applications.
