Response and assembly of abundant and rare taxa in Zaopei under different combination patterns of Daqu and pit mud:from microbial ecology to Baijiu brewing microecosystem

The quality and aroma of strong-flavor Baijiu are mainly dependent on Daqu,pit mud(PM),and the interaction of both.However,little is known about how their combination patterns affect the microbiome and metabolome of Zaopei,especially the metabolic function of rare taxa.Here,an experiment on industrial size was designed to assess the effects of 6 combinations(3 kinds of Daqu×2 kinds of PM)on the composition and assembly of different taxa,as well as the flavor profile.The results showed that Zaopei's microbiota was composed of a few abundant taxa and enormous rare taxa,and rare bacterial and abundant fungal subcommunities were significantly affected by combination patterns.The assembly processes of abundant/rare taxa and bacterial/fungal communities were distinct,and environmental changes mediated the balance between stochastic and deterministic processes in rare bacteria assembly.Furthermore,specific combination patterns improved the flavor quality of Zaopei by enhancing the interspecies interaction,which was closely related to rare taxa,especially rare bacteria.These findings highlighted that rare bacteria might be the keystone in involving community interaction and maintaining metabolic function,which provided a scientific foundation for better understanding and regulating the brewing microbiota from the viewpoint of microbial ecology.

Exploration of Therapeutic Measures and Clinical Efficacy for Recurrent Respiratory Infections in Children

Objective:To explore the treatment strategies and clinical effects for recurrent respiratory infections in children.Methods:From May 2022 to May 2024,100 pediatric patients with recurrent respiratory infections were selected in this study and evenly divided into two groups.The control group(50 patients)was treated with conventional therapy supplemented with budesonide,while the observation group(50 patients)received pidotimod treatment in addition to the control group’s treatment.Subsequently,the duration of clinical symptom improvement,respiratory function enhancement,serological index changes,reinfection status,and parental satisfaction were compared between the two groups.Results:In terms of clinical symptoms,the observation group showed significantly shorter durations of fever reduction,cough relief,tonsil swelling reduction,and disappearance of fine wet rales compared to the control group(average reduction times were 1.6 days,2.3 days,2.1 days,and 1.9 days,respectively,P<0.05).Regarding respiratory function,the observation group experienced a 12%increase in peak expiratory flow rate variability,a 0.6-liter increase in lung capacity,a 0.7-liter increase in forced lung capacity,and a 0.5-liter increase in forced expiratory volume in the first second after treatment,all significantly higher than the control group(P<0.05).Serological testing revealed that interferon-γand interleukin-2 levels increased by 15%and 18%,respectively,while interferon-α,interleukin-5,and interleukin-4 levels decreased by 10%,12%,and 9%,respectively,in the observation group,showing significant differences compared to the control group(P<0.05).Additionally,the reinfection rate in the observation group(10%)was significantly lower than that in the control group(30%),with an average reduction of two reinfections within one year and a 3.2-day shorter infection control time(P<0.05).In terms of parental satisfaction,the observation group achieved 95%,significantly higher than the 70%in the control group(P<0.05).Conclusion:The addition of pidotimod to conventional therapy for pediatric patients with recurrent respiratory infections can significantly alleviate clinical symptoms,promote the recovery of respiratory function,regulate serological indicators,effectively reduce the risk of reinfection,and improve parental satisfaction.This method deserves widespread clinical application.

Effects of biochar produced from distiller grains on agronomic performances of sorghum(Sorghum bicolor L.)and greenhouse gas emissions from soil

Aromatic liquor breweries produce massive distiller grains containing a high percentage of rice husks that necessitate harmless treatment and resource utilization.These husk-rich distiller grains can be pyrolyzed in the Ni-based catalyst system at a relatively low temperature(480?C)into combustible gas,which is used in liquor distillation,and biochar(BDG)with high mineral nutrients and good surface properties.A 3-year field experiment(2018-2020)was established to understand the effects of BDG on sorghum agronomic performances and greenhouse gas emissions from the soil.The results showed that BDG had higher mineral nutrient(N,P,K,and S)contents,larger cation exchange capacity,and better surface structure than those prepared using the traditional method at 400 and 600?C.Compared with sole chemical fertilizer(CF),the combination of CF and BDG(CF+BDG)increased sorghum nutrient(N,P,and K)uptake,yield,fertilizer use efficiency,and economic benefit.Cumulative CO2 emission from the soil changed little between with and without BDG,indicating the microbial stability of BDG.The effective adsorption of NH3 or NH4+by BDG upon N application may reduce N loss through NH3 and N2O emissions and increase the efficiency of fertilizer N use.Cumulative CH4 emission ranged from 32.45 to 44.86 g ha-1,which could be overlooked as a greenhouse gas in the sorghum field.Moreover,CF+BDG significantly decreased NH3 and N2O emissions for the production of each unit of sorghum grains and the CO2 emission from the land for the production of a certain amount of sorghum grains.Therefore,CF+BDG exhibited better agronomic and environmental performances in sorghum cultivation.

Modulation of expression of P16 and Her2 in rat breast tissues of mammary hyperplasia model by external use of Rupifang Extract

tract in external use on expression of proto-onco- genes her2 and tumor suppression genes p16 in rat breast tissues of mammary hyperplasia model. To explore the mechanisms of Rupifang Extract in external use for preventing and treating mammary hyperplasia. METHODS Thirty virginal female Wistar rats were randomized into 5 groups, 6 in each, A： blank con- trol group; B： model group; C： the low dose group of Rupifang; D： the middle dose group of Rupifang; and E： The high dose group of Rupifang. The mam- mary hyperplasia rat models were produced by in- jecting estradiol benzoate and progesterone and ir- ritating by tail nipping. Drug intervention was also launched during the model formation. After 30 days, the expression of her2 and p16 in breast tis- sues of rats in each group were detected by the SP immunohistochemical method. RESULTS： Compared with Blank control group, the expression of her2 in breast tissues in Model group was higher, and the expression of p16 was lower （P〈O.05 or P〈O.01）. After intervention with Rupi- fang Extract, compared with Model group, the ex- pression of her2 in breast tissues in Rupifang groups was lower, and the expression of p16 higher （P〈O.05 or P〈O.01）. CONCLUSION： The mechanisms of Rupifang Ex- tract in external application for preventing and treating mammary hyperplasia may be reducing the expression of proto-oncogenes her2 and in- creasing the expression of tumor suppression genes p16.

Reaction decoupling in thermochemical fuel conversion and technical progress based on decoupling using fluidized bed

Thermochemical conversion of fuels via pyrolysis/carbonization,cracking,gasification and combustion has to involve a number of individual reactions called attribution reactions to form an intercorrelated reaction network for any conversion process.By separating one or some attribution reactions from the others to decouple their interactions existing in the reaction network,the so-called reaction decoupling enables a better understanding of the complex thermal conversion process and further the optimization of the conditions for attribution reactions as well as the entire conversion process to realize advanced performances.The dual bed conversion and two-stage conversion are the two representative types of fuel conversion technologies developed in recent years based on reaction decoupling.Many technical advantages have been proven for such decoupling fuel conversion technologies,such as poly-generation of products,low-cost production of high-grade products,elimination of undesirable products or pollutants,easy operation and control,and so on.The treated fuels with decoupling conversion technologies mainly include solid biomass and coal,as well as liquid petroleum oil.This paper is devoted to reiteration of the reaction decoupling concept and further to reviewing the research,developments and successful applications of several decoupling fuel conversion technologies of two such types by using fluidized bed as their major reactors.

Ethyl esters enhancement of Jinchuan pear wine studied by coculturing Saccharomyces bayanus with Torulaspora delbrueckii and their community and interaction characteristics

To enhance the fruity flavour of pear wine by coculturing Saccharomyces yeasts with non-Saccharomyces,the coculture patterns were optimised based on the effect of different strains on the physicochemical properties and metabolites,and the interaction relationship in the coculture fermentation was also studied.These results showed that total volatiles of S.bayanus were higher than those of S.cerevisiae,especially esters,acids and phenols.The contents of total volatiles and esters were enhanced by sequential inoculation.The effect of coculturing S.bayanus Y4 with T.delbrueckii Y7 or Pichia sp.was better than that of coculturing with them with S.cerevisiae Y1,enhancing the contents of phenylethanol,acetate and ethyl esters.Furthermore,the esters(mainly ethyl esters)content was increased by 53.30%,and the acids content was decreased by 32.82%when S.bayanus Y4 was cocultured with T.delbrueckii Y7 by sequential inoculation compared with S.bayanus Y4 single inoculation.Their coculture increased theα-diversity of the fungal community and changed the correlation network between the microbiota.Redundancy analysis showed that Torulaspora was closely correlated to ethyl hexanoate,ethyl decanoate and ethyl 9-decenoate,while Saccharomyces was associated with hexanoic acid and octanoic acid.PICRUSt2 prediction revealed that Torulaspora may utilize the fatty acids produced by Saccharomyces to synthesize fatty acid ethyl esters.