乡村振兴视域下甘南藏区传统民居文化变迁研究

对于甘南藏区传统民居的研究,不仅需要信息技术层面的解读和诠释,更需要从文化的角度进行理解和释读。赋有文化内涵的居所是人类社会参与自然界的有力见证,也是一个民族历史文化传承的记忆载体,更是检验时代变迁过程中人们生活幸福指数的最佳平台。乡村振兴战略目标背景下甘南藏区村舍村貌改造工程离不开对其传统民居文化初衷及其出路的探讨,在此基础上传统民居的改迁,不仅能够留住乡愁,更有望实现各美其美、美人之美、美美与共、天下大同的美丽乡村,美丽中国的最终目标。

5-Acetyl-6,7,8,4′-tetramethylnortangeretin induces apoptosis in multiple myeloma U266 cells

Multiple myeloma(MM)is one of the most common hematological malignancies and to date,it remains an incurable disease.In this study,we evaluated the inhibitory effect of 5-acetyloxy-6,7,8,4′-tetramethoxyflavone(5-ATMF),a compound from aged citrus peel extracts,on the MM U266 cell line.We found that the compound inhibited cell growth and induced cell apoptosis in multiple apoptotic assays.The apoptotic proteins caspase-3,caspase-9,and PARP(poly ADP-ribose polymerase)were cleaved when cells were treated with 5-ATMF.Along with the apoptosis process,the anti-apoptotic protein,Bcl-2(B-cell lymphoma-2),was significantly downregulated and the pro-apoptotic protein,Bax(Bcl-2 associated X protein),upregulated along with the release of cytochrome C(Cyt c)and the reduction of mitochondrial membrane potential(MMP).Notably,we found that the phosphorylation of Bad(Bcl-2/Bcl-XL-associated death promoter)was decreased but Bad remained unchanged in this process.On adding 5-ATMF to U266 cells,we observed that 5-ATMF repressed the phosphorylation of Akt(PKB Protein Kinase B PKB),thereby increasing the release of Cyt c and inhibiting the phosphorylation of Bad.These effects were enhanced by Ly294002,an inhibitor of Akt.These results suggested that 5-ATMF exerts a pro-apoptotic effect on U266 cells,possibly associated with the mitochondrial apoptotic pathway induced by the PI3K/Akt/Bad pathway.

Characteristics of Sources and Sinks of Yugu 18

[Objectives] The study aimed to discuss the high-yield mechanism of Yugu 18.[Methods] The characteristics of sources and sinks of Yugu 18 with high quality, high yield, wide adaptability and multi-resistance to diseases were studied by using Jigu 18 as the control variety.[Results] Yugu 18 was superior in sink capability and the ability to fill sinks. In the filling period, its photosynthetic capacity was strong, and grain-leaf ratio was high. Photosynthetic products had a great contribution to grain filling after flowering, and the coordination between sources and sinks was good.[Conclusions] The research provides theoretical basis for the breeding and cultivation of high-yield foxtail millet.

Impaired glymphatic system as evidenced by low diffusivity along perivascular spaces is associated with cerebral small vessel disease:a population-based study

Objective This study aims to investigate the associations of glymphatic system with the presence,severity and neuroimaging phenotypes of cerebral small vessel disease(CSVD)in a community-based population.Method This report included 2219 community-dwelling people aged 50-75 years who participated in the PolyvasculaR Evaluation for Cognitive Impairment and vaScular Events cohort.The diffusivity along perivascular spaces based on diffusion tensor imaging(DTI-ALPS index)was measured to assess glymphatic pathway.The presence and severity of CSVD were estimated using a CSVD score(points from 0 to 6)and a modified CSVD score(points from 0 to 4),which were driven by 4 neuroimaging features of CSVD,including white matter hyperintensity(WMH),enlarged perivascular spaces(EPVS),lacunes,cerebral microbleeds.Brain atrophy(BA)was also evaluated.Binary or ordinal logistic regression analyses were carried out to investigate the relationships of DTI-ALPS index with CSVD.Result The mean age was 61.3(SD 6.6)years,and 1019(45.9%)participants were men.The average DTI-ALPS index was 1.67±0.14.Individuals in the first quartile(Q1)of the DTI-ALPS index had higher risks of the presence of CSVD(OR 1.77,95%CI 1.33 to 2.35,p<0.001),modified presence of CSVD(odds ratio(OR)1.80,95%CI 1.38 to 2.34,p<0.001),total burden of CSVD(common OR(cOR)1.89,95%CI 1.43 to 2.49,p<0.001)and modified total burden of CSVD(cOR 1.95,95%CI 1.51 to 2.50,p<0.001)compared with those in the fourth quartile(Q4).Additionally,individuals in Q1 of the DTI-ALPS index had increased risks of WMH burden,modified WMH burden,lacunes,basal ganglia-EPVS and BA(all p<0.05).Conclusion A lower DTI-ALPS index underlay the presence,severity and typical neuroimaging markers of CSVD,implying that glymphatic impairment may interact with CSVD-related pathology in the general ageing population.

PolyvasculaR Evaluation for Cognitive Impairment and vaScular Events (PRECISE)-a population-based prospective cohort study: rationale, design and baseline participant characteristics

Background and purpose Cardiovascular diseases and dementia are two major diseases in the elderly.Atherosclerosis is associated with future vascular events and cognitive impairment.The PolyvasculaR Evaluation for Cognitive Impairment and vaScular Events(PRECISE)study is a population-based prospective cohort study with comprehensive evaluation of multiterritorial artery stenosis and plaque using advanced vascular imaging techniques and prospective collection of vascular events and cognitive assessments.Methods Between May 2017 and September 2019,the PRECISE study enrolled 3067 community-dwelling adults with ages between 50 and 75 years cluster sampled from six villages and four communities of Lishui city in China.Data are collected in face-to face interviews at baseline,2-year and 4-year follow-up visits.Brain MRI including high-resolution sequences for intracranial and carotidal arteries and CT angiography for thoracoabdominal arteries were performed at baseline and will be rescanned after 4 years.Cardiovascular/cerebrovascular events and cognitive assessment will be prospectively collected after the enrollment.Blood and urine samples were collected and biomarkers were tested at baseline.Results A total of 3067 subjects were enrolled,among which 53.5%were female with an average age of 61.2±6.7 years.Among them,2.8%,8.1%,43.1%and 21.6%had a history of stroke,coronary heart diseases,hypertension and diabetes mellitus,respectively.Conclusions The PRECISE study is a population-based prospective cohort study with comprehensive evaluation of atherosclerotic stenosis and plaque using advanced vascular imaging techniques.Data from this cohort provide us an opportunity to precisely evaluate polyvascular atherosclerosis and its association with future vascular events and cognitive impairment.