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Heterologous expression of LamA gene encoded endo-β-1,3- glucanase and CO2 fixation by bioengineered Synechococcus sp. PCC 7002 被引量:1
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作者 Di Li swati yewalkar +7 位作者 Xiaotao Bi Sheldon Duff Dusko Posarac Heli Wang Layne A. Woodfin Jan-Hendrik Hehemann Sheila C. Potter Francis E. Nano 《Frontiers of Environmental Science & Engineering》 SCIE EI CAS CSCD 2017年第2期115-122,共8页
The gene for the catalytic domain of thermostable endo-β-1,3-glucanase (laminarinase) LamA was cloned from Thermotoga maritima MSB8 and heterologously expressed in a bioengineered Synechococcus sp. PCC 7002. The mu... The gene for the catalytic domain of thermostable endo-β-1,3-glucanase (laminarinase) LamA was cloned from Thermotoga maritima MSB8 and heterologously expressed in a bioengineered Synechococcus sp. PCC 7002. The mutant strain was cultured in a photobioreactor to assess biomass yield, recombinant laminarinase activity, and CO2 uptake. The maximum enzyme activity was observed at a oH of 8.0 and a temoerature of 70℃. At a CO2 concentration of 5%, we obtained a maximum specific growth rate of 0.083 h^-1 a biomass productivity of 0.42 g· L^-1·d^-1 a blomass concentration of 3.697 g.L^-1 , and a specific enzyme activity of the mutant strain of 4.325 U.mg^- 1 dry mass. All parameters decreased as CO2 concentration increased from 5% to 10% and further to 15% CO2, except enzyme activity, which increased from 5% to 10% CO2. However, the mutant culture still 1 1 grew at 15% CO2 concentration, as reflected by the blomass productwlty (0.26 g.L .d ), biomass concentration (2.416 g.L^- 1), and specific enzyme activity (3.247 U.mg^-1 dry mass). 展开更多
关键词 Synechococcus sp. PCC 7002Thermotoga maritimaLamA geneEndo-β-1 3-glucanaseCO2 fixation
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Applicability of differential fluorescein diacetate and propidium iodide fluorescence staining for monitoring algal growth and viability
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作者 swati yewalkar Tong Wu +6 位作者 David Kuan Heli Wang Di Li Andy Johnson Dusko Posarac Sheldon Duff Xiaotao T.Bi 《Waste Disposal and Sustainable Energy》 2019年第3期199-206,共8页
Microalgae can be cultivated for producing high-valued products through the production of enzymes to offset the cost of CO_(2) sequestration,providing financial incentives.The viability of algae in the photobioreactor... Microalgae can be cultivated for producing high-valued products through the production of enzymes to offset the cost of CO_(2) sequestration,providing financial incentives.The viability of algae in the photobioreactor needs to be monitored to ensure biologically active live cells.In this study,we explored a simple fluorometry method for differentiation of live and dead algal cells in photobioreactors by fluorescein diacetate(FDA)and propidium iodide(PI)fluorescence staining.FDA stains fluorescent green to the living cells while PI stains the dead cells,allowing the discrimination of live and dead cells.The method was evaluated using two green algae and two strains of cyanobacteria grown in shake flasks and a continuously stirred photobioreactor.The method was found applicable for Chlorella pyrenoidosa and Synechococcus 7002 but was not applicable for the cultures of Scenedesmus dimorphus and Synechococcus elongatus 7942.We conclude that FDA is a good stain for monitoring live algal cells in photobioreactors but its applicability to individual species of algae must be evaluated. 展开更多
关键词 Fluorescein diacetate(FDA) Propidium iodide(PI) Live and dead algae Differential staining Continuous photobioreactor
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