Alterations in hepatic metabolism of adult male rats following exposure to hydroxyprogesterone during embryonic development

Aim:To investigate the effect of in utero exposure to hydroxyprogesterone(HP)on liver metabolism in adult male albino rats.Methods:Pregnant Wistar strain albino rats were exposed to supra-normal levels(10 mg/kg and 25 mg/kg)of HP on days 1,7 and 14 of pregnancy.The male pups were maintained under controlled conditions and the rats were killed 90 days after birth.The liver tissue was immediately excised,weighed and used for biochemical assays. Results:The activity levels of succinate dehydrogenase(SDH),glutamate dehydrogenase(GDH),glucose-6-phosphate dehydrogenase(G-6-PDH),malate dehydrogenase(MDH)and aminotransaminases were significantly increased in the livers of rats exposed to HP during embryonic development.The lactate dehydrogenase(LDH)activity level was significantly decreased in the liver of experimental rats.Furthermore,there was a significant elevation of activity levels of antioxidant enzymes(glutathione S-transferase[GST] and catalase[CAT])with an increased lipid peroxidation in the hepatic tissue of experimental rats compared with the control group.Conclusion:The results of the present study suggest that there is an increase in the oxidative metabolism,antioxidative mechanism and levels of lipid peroxidation in rats exposed to HP during embryonic development.The increased aminotransaminase activities in these rats reveal tissue damage and disruption of mitochondrial integrity.(Asian J Androl 2006 Jul;8:463-467)