Recently,metal-organic framework (MOF)@enzyme composites have attracted increasing research interest.However,the fabrication of polymers-modified MOF@enzyme composites with high bio-catalytic performance remains chall...Recently,metal-organic framework (MOF)@enzyme composites have attracted increasing research interest.However,the fabrication of polymers-modified MOF@enzyme composites with high bio-catalytic performance remains challenging.Herein,a stimulus-responsive polymer,poly(acrylate-3',3'-dimethyl-6-nitro-spiro-2H-1-benzopyran-2,2' indoline-1'-ester-co-N-isopropyl acrylamide) (PSPN);was in-situ polymerized in UiO-66-NH2 (UN).The dual-responsive PSPN-UN@L-ASNase composites were constructed following L-asparaginase (L-ASNase) covalently attached to the UN surface.Interestingly,under 365 nm UV irradiation at 45 ℃,the PSP moiety in PSPN underwent a trans-to-cis conformational change and the PN moiety in PSPN transferred from a stretched- to a coiled-state,generating a confinement effect that significantly enhanced the bio-catalytic performance of the composites.Compared with free L-ASNase,the composites showed a 42.0-folds increase in maximum catalytic reaction velocity.Furthermore,the PSPN-UN@L-ASNase composites demonstrated high toxicity for Jurkat leukaemia cells.The stimulus-responsive polymer-MOF@enzyme composites provide a novel avenue for controlled bio-catalysis with great potential for targeted leukaemia therapy.展开更多
基金supported by the National Natural Science Foundation of China (No.22274159)。
文摘Recently,metal-organic framework (MOF)@enzyme composites have attracted increasing research interest.However,the fabrication of polymers-modified MOF@enzyme composites with high bio-catalytic performance remains challenging.Herein,a stimulus-responsive polymer,poly(acrylate-3',3'-dimethyl-6-nitro-spiro-2H-1-benzopyran-2,2' indoline-1'-ester-co-N-isopropyl acrylamide) (PSPN);was in-situ polymerized in UiO-66-NH2 (UN).The dual-responsive PSPN-UN@L-ASNase composites were constructed following L-asparaginase (L-ASNase) covalently attached to the UN surface.Interestingly,under 365 nm UV irradiation at 45 ℃,the PSP moiety in PSPN underwent a trans-to-cis conformational change and the PN moiety in PSPN transferred from a stretched- to a coiled-state,generating a confinement effect that significantly enhanced the bio-catalytic performance of the composites.Compared with free L-ASNase,the composites showed a 42.0-folds increase in maximum catalytic reaction velocity.Furthermore,the PSPN-UN@L-ASNase composites demonstrated high toxicity for Jurkat leukaemia cells.The stimulus-responsive polymer-MOF@enzyme composites provide a novel avenue for controlled bio-catalysis with great potential for targeted leukaemia therapy.
