The molecular phylogenetic trees of 10 species representing 6 genera of the family Rhizophoraceae have been constructed using the sequences of chloroplast genes ma/K and rbcL as well as the ITS regions of nuclear ribo...The molecular phylogenetic trees of 10 species representing 6 genera of the family Rhizophoraceae have been constructed using the sequences of chloroplast genes ma/K and rbcL as well as the ITS regions of nuclear ribosomal DMA. Relative-rate tests between lineages in these phylogenetic trees have been performed. On the basis of the results of the relative-rate tests and related molecular evolutionary rate data, the divergence times between the lineages are estimated as follows: ( i) the first divergence time in these genera is 132.25 million years ago (mya); (ii) the average divergence time between two tribes, i.e. inland Legnotideae (except Carallia brachiata) and mangrove Rhizophoreae, is 64.13 mya; and (iii) the average divergence time between two inland species, C. garciniaefolia and C. pectinifolia, is 19.92 mya.展开更多
A simple method for preparation of template DNA suitable for PCR amplification from herbarium samples and plant tissues rich in byproducts, e.g. polysaccha-rides, tannins, polyphenolic, and terpenoids compounds, is de...A simple method for preparation of template DNA suitable for PCR amplification from herbarium samples and plant tissues rich in byproducts, e.g. polysaccha-rides, tannins, polyphenolic, and terpenoids compounds, is described. The total DNA from regular extraction procedure is absorbed by a small amount of glass powder and the final precipitation of glass powder is used directly as a template for PCR. Taking six plant taxa, including the herbarium specimens of Lythraceae collected from Namibia in 1957 and the silicon-dried leaf tissue from mangrove plants (Rhizo-phoraceae and Combretaceae) rich in by-products as examples, the PCR products, including nrDNA ITS regions and cpDNA rbcL gene, amplified following the regular and new methods respectively are compared. Our method provides a simple, rapid and economic approach to purify and prepare template DNA for PCR from special plant materials.展开更多
文摘The molecular phylogenetic trees of 10 species representing 6 genera of the family Rhizophoraceae have been constructed using the sequences of chloroplast genes ma/K and rbcL as well as the ITS regions of nuclear ribosomal DMA. Relative-rate tests between lineages in these phylogenetic trees have been performed. On the basis of the results of the relative-rate tests and related molecular evolutionary rate data, the divergence times between the lineages are estimated as follows: ( i) the first divergence time in these genera is 132.25 million years ago (mya); (ii) the average divergence time between two tribes, i.e. inland Legnotideae (except Carallia brachiata) and mangrove Rhizophoreae, is 64.13 mya; and (iii) the average divergence time between two inland species, C. garciniaefolia and C. pectinifolia, is 19.92 mya.
基金This work was supported by the Chinese National Science Funds for Distinguished Young Scholar (Grant No. 39825104)the National Natural Science Foundation of China (Grant Nos. 39970057 and 30170071) the Natural Science Foundation of Guangdong Provinc
文摘A simple method for preparation of template DNA suitable for PCR amplification from herbarium samples and plant tissues rich in byproducts, e.g. polysaccha-rides, tannins, polyphenolic, and terpenoids compounds, is described. The total DNA from regular extraction procedure is absorbed by a small amount of glass powder and the final precipitation of glass powder is used directly as a template for PCR. Taking six plant taxa, including the herbarium specimens of Lythraceae collected from Namibia in 1957 and the silicon-dried leaf tissue from mangrove plants (Rhizo-phoraceae and Combretaceae) rich in by-products as examples, the PCR products, including nrDNA ITS regions and cpDNA rbcL gene, amplified following the regular and new methods respectively are compared. Our method provides a simple, rapid and economic approach to purify and prepare template DNA for PCR from special plant materials.
