AIM: To study the chemical constituents of the solid culture of the endophyte Phomopsis sp. IFB-E060 in Vatica mangachapoi. METHOD: Isolation and purification were performed through silica gel column chromatography, g...AIM: To study the chemical constituents of the solid culture of the endophyte Phomopsis sp. IFB-E060 in Vatica mangachapoi. METHOD: Isolation and purification were performed through silica gel column chromatography, gel filtration over Sephadex LH-20, ODS column chromatography, and HPLC. Structures of the isolated compounds were elucidated by a combination of spectroscopic analyses(UV, CD, IR, MS, 1D, and 2D NMR). The cytotoxicity of the isolates was evaluated in vitro by the MTT method against the human hepatocarcinoma cell line SMMC-7721. RESULTS: Five compounds were isolated from the solid culture of the endophyte Phomopsis sp. IFB-E060 and their structures were identified as 18-methoxy cytochalasin J(1), cytochalasin H(2),(22E, 24S)-cerevisterol(3), ergosterol(4), and nicotinic acid(5). Compound 1 had an inhibition rate of 24.4% at 10 μg·mL-1 and 2 had an IC50 value of 15.0 μg·mL-1, while a positive control 5-fluorouracil had an inhibition rate of 28.7% at 10 μg·mL-1. CONCLUSION: 18-Methoxy cytochalasin J(1), produced by endophytic Phomopsis sp. IFB-E060, is a new cytochalasin with weak cytotoxicity to the human hepatocarcinoma cell line SMMC-7721.展开更多
Three new phenazine-type compounds, named phenazines SA–SC(1–3), together with four new natural products(4–7), were isolated from the fermentation broth of an earwig-associated Streptomyces sp. NA04227. The structu...Three new phenazine-type compounds, named phenazines SA–SC(1–3), together with four new natural products(4–7), were isolated from the fermentation broth of an earwig-associated Streptomyces sp. NA04227. The structures of these compounds were determined by extensive analyses of NMR, high resolution mass spectroscopic data, as well as single-crystal X-ray diffraction measurement. Sequencing and analysis of the genome data allowed us to identify the gene cluster(spz) and propose a biosynthetic pathway for these phenazine-type compounds. Additionally, compounds 1–5 exhibited moderate inhibitory activity against acetylcholinesterase(AChE), and compound 3 showed antimicrobial activities against Micrococcus luteus.展开更多
Six new(1–6)and seven known depsidones(7–13)were isolated from the culture of an ant(Monomorium chinensis)-derived fungus Spiromastix sp.MY-1.Their structures were elucidated by extensive spectroscopic analysis incl...Six new(1–6)and seven known depsidones(7–13)were isolated from the culture of an ant(Monomorium chinensis)-derived fungus Spiromastix sp.MY-1.Their structures were elucidated by extensive spectroscopic analysis including high resolution MS,1D and 2D NMR data.The new bromide depsidones were obtained through supplementing potassium bromide in the fermentation medium of Spiromastix sp.MY-1.All isolated compounds showed various bioactivities against the tested phytopathogenic bacteria.Particularly,new bromide compound 4,named spiromastixone S,exhibited the strongest activity against Xanthomonas oryzae pv.oryzae with a MIC value of 5.2μmol·^L(−1).展开更多
Replacement of the native promoter of the global regulator Lae A-like gene of Daldinia eschscholzii by a strong gpdA promoter led to the generation of two novel cyclopentenone metabolites, named dalestones A and B, wh...Replacement of the native promoter of the global regulator Lae A-like gene of Daldinia eschscholzii by a strong gpdA promoter led to the generation of two novel cyclopentenone metabolites, named dalestones A and B, whose structures were assigned by a combination of spectroscopic analysis, modified Mosher's reaction, and electronic circular dichroism(ECD). Dalestones A and B inhibit the gene expression of TNF-α and IL-6 in LPS-induced RAW264.7 macrophages.展开更多
Two new 2-carboxymethyl-3-hexyl-maleic anhydride derivatives,arthrianhydride A(1)and B(2),along with three known compounds 3-5,were isolated from the fermentation broth of a grasshopper-associated fungus Arthrinium sp...Two new 2-carboxymethyl-3-hexyl-maleic anhydride derivatives,arthrianhydride A(1)and B(2),along with three known compounds 3-5,were isolated from the fermentation broth of a grasshopper-associated fungus Arthrinium sp.NF2410.The structures of new compounds 1 and 2 were determined based on the analysis of the HR-ESI-MS and NMR spectroscopic data.Furthermore,compounds 1 and 2 were evaluated on inhibitory activity against the enzyme SHP2 and both of them showed moderate inhibitory activity against SHP2.展开更多
基金supported by the Natural Science Foundation of China(Nos.81121062,30701045,21132004&21072092)the Natural Science Foundation of Jiangsu Province(No.BK2009010)the Social Development Project of Yangzhou(No.YZ2011097)
文摘AIM: To study the chemical constituents of the solid culture of the endophyte Phomopsis sp. IFB-E060 in Vatica mangachapoi. METHOD: Isolation and purification were performed through silica gel column chromatography, gel filtration over Sephadex LH-20, ODS column chromatography, and HPLC. Structures of the isolated compounds were elucidated by a combination of spectroscopic analyses(UV, CD, IR, MS, 1D, and 2D NMR). The cytotoxicity of the isolates was evaluated in vitro by the MTT method against the human hepatocarcinoma cell line SMMC-7721. RESULTS: Five compounds were isolated from the solid culture of the endophyte Phomopsis sp. IFB-E060 and their structures were identified as 18-methoxy cytochalasin J(1), cytochalasin H(2),(22E, 24S)-cerevisterol(3), ergosterol(4), and nicotinic acid(5). Compound 1 had an inhibition rate of 24.4% at 10 μg·mL-1 and 2 had an IC50 value of 15.0 μg·mL-1, while a positive control 5-fluorouracil had an inhibition rate of 28.7% at 10 μg·mL-1. CONCLUSION: 18-Methoxy cytochalasin J(1), produced by endophytic Phomopsis sp. IFB-E060, is a new cytochalasin with weak cytotoxicity to the human hepatocarcinoma cell line SMMC-7721.
基金supported by Ministry of Science and Technology of China(No.2018YFC1706205)the Natural Science Foundation of China(Nos.81522042,21572100,81773591,81530089,21672101,81673333,21861142005,21761142001,21661140001,J1210026,and J1103512)+2 种基金Fundamental Research Funds for the Central Universities(Nos.020814380092 and 020814380103)Central Public-interest Scientific Institution Basal Research Fund for Chinese Academy of Tropical Agricultural Sciences(No.1630052019011 and 19CXTD-32)the Financial Fund of the Ministry of Agriculture and Rural Affairs,China(No.NFZX-2018)
文摘Three new phenazine-type compounds, named phenazines SA–SC(1–3), together with four new natural products(4–7), were isolated from the fermentation broth of an earwig-associated Streptomyces sp. NA04227. The structures of these compounds were determined by extensive analyses of NMR, high resolution mass spectroscopic data, as well as single-crystal X-ray diffraction measurement. Sequencing and analysis of the genome data allowed us to identify the gene cluster(spz) and propose a biosynthetic pathway for these phenazine-type compounds. Additionally, compounds 1–5 exhibited moderate inhibitory activity against acetylcholinesterase(AChE), and compound 3 showed antimicrobial activities against Micrococcus luteus.
基金supported by the National Key Research and Development Programs(Nos.2018YFA0902000 and 2017YFC0506200)the National Natural Science Foundation of China(Nos.81991524 and 81673333)+1 种基金the Hainan Provincial Basic and Applied Basic Research Fund for High-Level Talents in Natural Science(Nos.2019RC306 and 2019RC352)the Central Public-interest Scientific Institution Basal Research Fund for CATAS-ITBB(Nos.1630052019011 and 1630052022016)。
文摘Six new(1–6)and seven known depsidones(7–13)were isolated from the culture of an ant(Monomorium chinensis)-derived fungus Spiromastix sp.MY-1.Their structures were elucidated by extensive spectroscopic analysis including high resolution MS,1D and 2D NMR data.The new bromide depsidones were obtained through supplementing potassium bromide in the fermentation medium of Spiromastix sp.MY-1.All isolated compounds showed various bioactivities against the tested phytopathogenic bacteria.Particularly,new bromide compound 4,named spiromastixone S,exhibited the strongest activity against Xanthomonas oryzae pv.oryzae with a MIC value of 5.2μmol·^L(−1).
基金supported by the National Natural Science Foundation of China(Nos.21672101,21702099 and 81673333)
文摘Replacement of the native promoter of the global regulator Lae A-like gene of Daldinia eschscholzii by a strong gpdA promoter led to the generation of two novel cyclopentenone metabolites, named dalestones A and B, whose structures were assigned by a combination of spectroscopic analysis, modified Mosher's reaction, and electronic circular dichroism(ECD). Dalestones A and B inhibit the gene expression of TNF-α and IL-6 in LPS-induced RAW264.7 macrophages.
基金supported by the Ministry of Science and Technology(Nos.2018YFC1706200 and 2018YFA.902000)the National Natural Science Foundation of China(Nos.81925033,21861142005,81773591,21761142001,81673333,21661140001,and 91853109)。
文摘Two new 2-carboxymethyl-3-hexyl-maleic anhydride derivatives,arthrianhydride A(1)and B(2),along with three known compounds 3-5,were isolated from the fermentation broth of a grasshopper-associated fungus Arthrinium sp.NF2410.The structures of new compounds 1 and 2 were determined based on the analysis of the HR-ESI-MS and NMR spectroscopic data.Furthermore,compounds 1 and 2 were evaluated on inhibitory activity against the enzyme SHP2 and both of them showed moderate inhibitory activity against SHP2.
