An acidic polysaccharide (PY3) was isolated from the hot water extract of the red algae Porphyra yezoensis by successive column chromatographies over DEAE-cellulose and Sephadex G-200. PY3 with an average molecular we...An acidic polysaccharide (PY3) was isolated from the hot water extract of the red algae Porphyra yezoensis by successive column chromatographies over DEAE-cellulose and Sephadex G-200. PY3 with an average molecular weight of 1.8×105 was demonstrated to be composed of galactose (Gal), 3,6-anhydrogalactose (3,6-AnGal), 6-OSO3-galactose (6-OSO3-Gal) and xylose (Xyl) in an approximate molar ratio of 25:15:10:1. In view of Smith degradation and me-thylation and on the basis of spectral evidence including those of IR, GC, GC-MS, and 1H and 13C NMR, the most probable repeating unit of PY3 could be proposed as [(1-3)β-D-Gal(1- 4)α-L-3,6-AnGal]3-[1-3)β-D-Gal(1-4)α-L-6-OSO3-Gal]2 with a xylose moiety at the C6 of one of every twenty-five β-D-Gal residues. To our knowledge, PY3 was shown to be the first porphyran possessing occasional xylose branches.展开更多
基金This work was supported by the National Natural Science Foundation of China (Grant Nos. 39725033 and 39970083)and also by the Research Fund for the Doctoral Program of Higher Education (Grant No. 1999028419).
文摘An acidic polysaccharide (PY3) was isolated from the hot water extract of the red algae Porphyra yezoensis by successive column chromatographies over DEAE-cellulose and Sephadex G-200. PY3 with an average molecular weight of 1.8×105 was demonstrated to be composed of galactose (Gal), 3,6-anhydrogalactose (3,6-AnGal), 6-OSO3-galactose (6-OSO3-Gal) and xylose (Xyl) in an approximate molar ratio of 25:15:10:1. In view of Smith degradation and me-thylation and on the basis of spectral evidence including those of IR, GC, GC-MS, and 1H and 13C NMR, the most probable repeating unit of PY3 could be proposed as [(1-3)β-D-Gal(1- 4)α-L-3,6-AnGal]3-[1-3)β-D-Gal(1-4)α-L-6-OSO3-Gal]2 with a xylose moiety at the C6 of one of every twenty-five β-D-Gal residues. To our knowledge, PY3 was shown to be the first porphyran possessing occasional xylose branches.
