Although some indica rice varieties have already been transformed by Agrobacterium,there is still great demand of developing transformation system for economically important indica varieties.Here,a fast and efficient ...Although some indica rice varieties have already been transformed by Agrobacterium,there is still great demand of developing transformation system for economically important indica varieties.Here,a fast and efficient method of Agrobacterium-mediated transformation for indica rice was developed,and an NcGDH transgenic genic male-sterile(GMS)indica rice 628S was generated using this method.The regenerated transgenic plantlets can be obtained within three months,and the transformation efficiency achieved about 17%.NcGDH transgenic 628S exhibited an enhanced capacity of ammonium assimilation and more effective panicles under low nitrogen conditions.Moreover the hybrid rice lines generated by NcGDH transgenic 628S showed 8%-27%yield advantage than those by the non-transgenic 628S.Taken together,these results provide an efficient transfbnnation method for indica rice and an opportunity to improve nitrogen use efficiency(NUE)and the heterosis of hybrid rice.展开更多
基金supported by the National Science Foundation of China(Grant Nos.31571635 and 31871595)Hunan Provincial Important Science and Technology Specific Projects(Grant No.2018NK1010)+2 种基金Natural Science Foundation of Hunan Province,China(Grant No.2020JJ4004)Public Subject of State Key Laboratory of Hybrid Rice(Hunan Hybrid Rice Research Center)(Grant No.2019KF02)China Postdoctoral Science Foundation(Grant No.2020M682561).
文摘Although some indica rice varieties have already been transformed by Agrobacterium,there is still great demand of developing transformation system for economically important indica varieties.Here,a fast and efficient method of Agrobacterium-mediated transformation for indica rice was developed,and an NcGDH transgenic genic male-sterile(GMS)indica rice 628S was generated using this method.The regenerated transgenic plantlets can be obtained within three months,and the transformation efficiency achieved about 17%.NcGDH transgenic 628S exhibited an enhanced capacity of ammonium assimilation and more effective panicles under low nitrogen conditions.Moreover the hybrid rice lines generated by NcGDH transgenic 628S showed 8%-27%yield advantage than those by the non-transgenic 628S.Taken together,these results provide an efficient transfbnnation method for indica rice and an opportunity to improve nitrogen use efficiency(NUE)and the heterosis of hybrid rice.
