目的基于网络药理学和分子对接方法探究“麦冬-五味子”药对治疗心肌纤维化的作用机制。方法运用TCMSP数据库及HERB数据库筛选“麦冬-五味子”药对药物成分和靶点,运用GeneCards数据库、Disgenet数据库、OMIM数据库筛选疾病靶点,获取共...目的基于网络药理学和分子对接方法探究“麦冬-五味子”药对治疗心肌纤维化的作用机制。方法运用TCMSP数据库及HERB数据库筛选“麦冬-五味子”药对药物成分和靶点,运用GeneCards数据库、Disgenet数据库、OMIM数据库筛选疾病靶点,获取共同靶点;通过Cytoscape软件构建“药物活性成分-疾病-靶点”网络模型;利用微生信平台进行基因本体论功能富集分析(gene ontology,GO)和京都基因与基因组百科全书富集分析(kyoto encyclopedia of genes and genomes,KEGG);利用STRING数据库构建蛋白互作网络;通过Auto Dock Tools1.5.6软件进行分子对接验证。结果“麦冬-五味子”药对中共筛选得到戈米辛M2、五味子酚乙、麦冬二氢高异黄酮B等85个活性成分,主要作用于HSP90AA1、AKT1、SRC等163个心肌纤维化靶点上,主要富集在脂质与动脉粥样硬化信号通路、糖尿病并发症中的age-rage信号通路、癌症发病途径信号通路等相关通路上。结论“麦冬-五味子”药对主要通过调控炎性反应、氧化应激、脂质沉积、细胞增殖凋亡等多种生物过程来抑制心肌纤维化。展开更多
The paper evaluates the changes in the ecological environment of cultivated land in the major crop-producing areas major crop-producing areas in the past 20 years by constructing an ecological pollution index;and quan...The paper evaluates the changes in the ecological environment of cultivated land in the major crop-producing areas major crop-producing areas in the past 20 years by constructing an ecological pollution index;and quantitatively analyzes the effects of the existing policies and their main influencing factors by applying the PSM-DID method based on the frequency of policy enactment.The results are as follows:first,the existing ecological compensation policies for cultivated land are effective but not significant in improving the ecological environment in the major crop-producing areas.The economic development level and grain cultivation area of the major crop-producing areas are significantly and positively correlated with the improvement of the arable land ecological environment,while the level of agricultural infrastructure construction is significantly and negatively correlated with the improvement of the arable land ecological environment.Second,the reason for the ineffectiveness of the current arable ecological land compensation policy in protecting the arable land ecological environment in the main grain-producing regions is that the existing arable ecological land compensation policy in China has its own institutional defects and needs to be further improved.At the same time,the level of environmental pollution of cultivated land in the main grain-producing regions is positively correlated with the level of income from cultivated land and cultivated area.展开更多
文摘目的基于网络药理学和分子对接方法探究“麦冬-五味子”药对治疗心肌纤维化的作用机制。方法运用TCMSP数据库及HERB数据库筛选“麦冬-五味子”药对药物成分和靶点,运用GeneCards数据库、Disgenet数据库、OMIM数据库筛选疾病靶点,获取共同靶点;通过Cytoscape软件构建“药物活性成分-疾病-靶点”网络模型;利用微生信平台进行基因本体论功能富集分析(gene ontology,GO)和京都基因与基因组百科全书富集分析(kyoto encyclopedia of genes and genomes,KEGG);利用STRING数据库构建蛋白互作网络;通过Auto Dock Tools1.5.6软件进行分子对接验证。结果“麦冬-五味子”药对中共筛选得到戈米辛M2、五味子酚乙、麦冬二氢高异黄酮B等85个活性成分,主要作用于HSP90AA1、AKT1、SRC等163个心肌纤维化靶点上,主要富集在脂质与动脉粥样硬化信号通路、糖尿病并发症中的age-rage信号通路、癌症发病途径信号通路等相关通路上。结论“麦冬-五味子”药对主要通过调控炎性反应、氧化应激、脂质沉积、细胞增殖凋亡等多种生物过程来抑制心肌纤维化。
文摘The paper evaluates the changes in the ecological environment of cultivated land in the major crop-producing areas major crop-producing areas in the past 20 years by constructing an ecological pollution index;and quantitatively analyzes the effects of the existing policies and their main influencing factors by applying the PSM-DID method based on the frequency of policy enactment.The results are as follows:first,the existing ecological compensation policies for cultivated land are effective but not significant in improving the ecological environment in the major crop-producing areas.The economic development level and grain cultivation area of the major crop-producing areas are significantly and positively correlated with the improvement of the arable land ecological environment,while the level of agricultural infrastructure construction is significantly and negatively correlated with the improvement of the arable land ecological environment.Second,the reason for the ineffectiveness of the current arable ecological land compensation policy in protecting the arable land ecological environment in the main grain-producing regions is that the existing arable ecological land compensation policy in China has its own institutional defects and needs to be further improved.At the same time,the level of environmental pollution of cultivated land in the main grain-producing regions is positively correlated with the level of income from cultivated land and cultivated area.
