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Comparative Proteomics Analysis of the Sieve Tube from Rubber Tree(Hevea brasiliensis) Seedlings under Contrasting Ethylene Treatments 被引量:2
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作者 DAI Long-jun QIN Yun-xia +5 位作者 YANG Hong DENG Zhi SHI Min-jing LIU Hui tang chao-rong LI De-jun 《Agricultural Science & Technology》 CAS 2019年第2期18-27,共10页
[Objective]To elucidate the role of ethylene(ET),a latex yield stimulant of the rubber tree,on the sieve tube(ST)transport efficiency of materials(especially sucrose)needed for natural rubber biosynthesis.[Method]Rubb... [Objective]To elucidate the role of ethylene(ET),a latex yield stimulant of the rubber tree,on the sieve tube(ST)transport efficiency of materials(especially sucrose)needed for natural rubber biosynthesis.[Method]Rubber tree seedlings were treated with ET solution or water which was used as a control on the bark,and latex samples and ST tissue samples were collected for proteomic analyses and latex sucrose content determination respectively.[Results]After ET treatment,the sucrose content of the latex was found significantly decreased.A total of 66 ethylene-responsive proteins(ERPs)were distinguished by two-dimensional gel electrophoresis(2-DE),and 54 were successfully identified by MALDI-TOF/TOF and database searching.The majority of these ERPs were involved in carbohydrate transport and metabolic processes in the ST.[Conclusion]Our findings suggest that the application of ET may increase the transport efficiency of the ST and that the application of ET promotes the consumption of energy and sucrose in the ST. 展开更多
关键词 Sieve tube Rubber tree Comparative proteomics Ethylene-responsive proteins Sucrose content
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