建立高分辨熔解曲线技术同时快速检测鸡肉中沙门氏菌、单增李斯特菌、金黄色葡萄球菌和空肠弯曲菌。本研究分别以上述4种菌的fimY、hly、nuc和orfC特异性基因为靶基因,建立能同时检测多种食源性致病菌的多重HRM-real time PCR检测体系,...建立高分辨熔解曲线技术同时快速检测鸡肉中沙门氏菌、单增李斯特菌、金黄色葡萄球菌和空肠弯曲菌。本研究分别以上述4种菌的fimY、hly、nuc和orfC特异性基因为靶基因,建立能同时检测多种食源性致病菌的多重HRM-real time PCR检测体系,并对反应的特异性、灵敏度、重复性以及在人工染菌样品进行了评价。所建立的HRM-real time PCR检测体系对沙门氏菌、单增李斯特菌、金黄色葡萄球菌和空肠弯曲菌均产生特异性熔解曲线,Tm值分别为82.2℃、78.2℃、75.5℃和73.5℃;多重HRM-real time PCR反应体系检测单一致病菌菌液的灵敏度均为102拷贝数/mL,检测多种致病菌菌液的灵敏度为102拷贝数/mL;该反应体系重复性的试验内CV值在0.03%~0.32%之间,试验间CV值在0.45%~2.12%;人工染菌样本对4种菌的检测限均能达到5 CFU/25 g。结果表明该多重HRM-real time PCR检测体系可对上述4种禽肉中常见致病菌进行有效的检测与区分,特异性强,灵敏度高,反应重复性好,有望在食源性致病菌检测分析中发挥作用。展开更多
[Objective]In order to compare the general egg qualities and mineral element contents of different layers,six commercial breeds in brown shell layer,including Lohmann,Jinghong,Hyline,Xinyang,Hisax,ISA,Suqing green she...[Objective]In order to compare the general egg qualities and mineral element contents of different layers,six commercial breeds in brown shell layer,including Lohmann,Jinghong,Hyline,Xinyang,Hisax,ISA,Suqing green shell layer and Wenchang chicken were used as materials.The general egg quality traits and mineral elements contents of eight breeds at 43 weeks old were mensurated.[Result] There were significant differences in general egg qualities（except yolk weight）of eight breeds.About the mineral element contents,the differences were significant only in P,Mn and Se of some breeds in this experiment.[Conclusion]The results in this study could provide the reference for further researcher,layer producers,nutritionists,consumers and so on.展开更多
文摘建立高分辨熔解曲线技术同时快速检测鸡肉中沙门氏菌、单增李斯特菌、金黄色葡萄球菌和空肠弯曲菌。本研究分别以上述4种菌的fimY、hly、nuc和orfC特异性基因为靶基因,建立能同时检测多种食源性致病菌的多重HRM-real time PCR检测体系,并对反应的特异性、灵敏度、重复性以及在人工染菌样品进行了评价。所建立的HRM-real time PCR检测体系对沙门氏菌、单增李斯特菌、金黄色葡萄球菌和空肠弯曲菌均产生特异性熔解曲线,Tm值分别为82.2℃、78.2℃、75.5℃和73.5℃;多重HRM-real time PCR反应体系检测单一致病菌菌液的灵敏度均为102拷贝数/mL,检测多种致病菌菌液的灵敏度为102拷贝数/mL;该反应体系重复性的试验内CV值在0.03%~0.32%之间,试验间CV值在0.45%~2.12%;人工染菌样本对4种菌的检测限均能达到5 CFU/25 g。结果表明该多重HRM-real time PCR检测体系可对上述4种禽肉中常见致病菌进行有效的检测与区分,特异性强,灵敏度高,反应重复性好,有望在食源性致病菌检测分析中发挥作用。
基金Supported by National Science and Technology Support Program (2012BAD39B04-3)Yangzhou city societal development item (YZ2010080)
文摘[Objective]In order to compare the general egg qualities and mineral element contents of different layers,six commercial breeds in brown shell layer,including Lohmann,Jinghong,Hyline,Xinyang,Hisax,ISA,Suqing green shell layer and Wenchang chicken were used as materials.The general egg quality traits and mineral elements contents of eight breeds at 43 weeks old were mensurated.[Result] There were significant differences in general egg qualities（except yolk weight）of eight breeds.About the mineral element contents,the differences were significant only in P,Mn and Se of some breeds in this experiment.[Conclusion]The results in this study could provide the reference for further researcher,layer producers,nutritionists,consumers and so on.
