IM To study the radioimmunoimaging (RAII) using the human/mouse chimeric Ab to evaluate its targeting activity in animal models.METHODS To chimeric Ab was labeled with 131I. RAII was performed at different intervals...IM To study the radioimmunoimaging (RAII) using the human/mouse chimeric Ab to evaluate its targeting activity in animal models.METHODS To chimeric Ab was labeled with 131I. RAII was performed at different intervals after injection of radiolabeled Abs in nude mice with human hepatoma xenograft, and tissue distribution of radioactivity was measured. Comparison was made in the chimeric Ab between the single segment Ab and previous murine mAb against HBxAg.RESULTS The experimental objects developed tumorpositive image after 2 days of radiolabeled Abs injection, and the peak accumulation of radioactivity fell on the 7th day. The tumor/liver ratioactivity of the chimeric Ab, single segment Ab, antiHBx mAb, and the control group was 281±021, 244±016, 460±019, and 096±014, respectively.CONCLUSION The genetic engineering Abs have a considerable targeting activity which can be used as a novel humanized vector in the targeting treatment of liver cancer..展开更多
Modern medicine has been fighting the battle with cancer for near 200 years, achieved substantial progresses but yet to claim the final victory. Author believes that the battle strategy has the profound impact to the ...Modern medicine has been fighting the battle with cancer for near 200 years, achieved substantial progresses but yet to claim the final victory. Author believes that the battle strategy has the profound impact to the result. Since Virchow discovered the cellular origin of cancer in 1863, cancers are being seeing as a localized disease resulting from foreign invasions whereas eradication therapies have been the dominant practices. However, people neglected the overall relationship between cancer and human body therefore overlooked the solutions from transformation, modulation and regulation point of view.展开更多
文摘IM To study the radioimmunoimaging (RAII) using the human/mouse chimeric Ab to evaluate its targeting activity in animal models.METHODS To chimeric Ab was labeled with 131I. RAII was performed at different intervals after injection of radiolabeled Abs in nude mice with human hepatoma xenograft, and tissue distribution of radioactivity was measured. Comparison was made in the chimeric Ab between the single segment Ab and previous murine mAb against HBxAg.RESULTS The experimental objects developed tumorpositive image after 2 days of radiolabeled Abs injection, and the peak accumulation of radioactivity fell on the 7th day. The tumor/liver ratioactivity of the chimeric Ab, single segment Ab, antiHBx mAb, and the control group was 281±021, 244±016, 460±019, and 096±014, respectively.CONCLUSION The genetic engineering Abs have a considerable targeting activity which can be used as a novel humanized vector in the targeting treatment of liver cancer..
文摘Modern medicine has been fighting the battle with cancer for near 200 years, achieved substantial progresses but yet to claim the final victory. Author believes that the battle strategy has the profound impact to the result. Since Virchow discovered the cellular origin of cancer in 1863, cancers are being seeing as a localized disease resulting from foreign invasions whereas eradication therapies have been the dominant practices. However, people neglected the overall relationship between cancer and human body therefore overlooked the solutions from transformation, modulation and regulation point of view.
