Objective:To investigate the intervention of Xuduan Zhongzi Formula on the epididymis structure of model of oligospermia mice.Methods:Ten of the 45 male mice were labeled as the normal group,and the remaining 35 mice ...Objective:To investigate the intervention of Xuduan Zhongzi Formula on the epididymis structure of model of oligospermia mice.Methods:Ten of the 45 male mice were labeled as the normal group,and the remaining 35 mice were injected with chloral hydrate for five consecutive days,and the randomly selected five mice were anesthetized with chloral hydrate.The left epididymis was removed,and a few sperm were found in the epididymis under the optical microscope,indicating successful construction of the model.They were randomly divided into three groups:the normal group,the L-carnitine group and the Xuduan group with 10 rats in each group.The the L-carnitine group and the Xuduan group were administrated with equivalent dose of human solvent,and the normal group and the model group were administrated with normal saline.After 8 weeks of intragastric administration,all experimental mice were killed,and the left epididymis was extracted for sperm detection,seminal plasma biochemical detection,HE staining,and electron microscopy.Results:In the model group,enlarged epididymal epithelial cells,vacuolar degeneration of primary and basal cells,and edema of interstitial cells and vascular dilation were found.Compared with the normal group,semen concentration,activity,SOD,A-glucosidase and fructose in the model group were significantly decreased,and the difference was statistically significant(P<0.01).After eight weeks of drug intragastric treatment,the semen concentration,activity,SOD,A-glucosidase and fructose in the Xuduan group were significantly increased compared with the model group,and the difference was statistically significant(P<0.01).Conclusion:Xuduan Zhongzi Formula can significantly improve the epididymal structure,the microenvironment of epididymal sperm maturation and the stability of epididymal epithelial structure of oligospermia model mice,creating conditions for sperm maturation in the epididymis.展开更多
脑机接口(brain-computer interface,BCI)的分类性能一定程度上取决于对脑电信号的预处理方法,这项研究提出了一种空域时域滤波的预处理方法,以解决人类视觉系统中的潜伏延迟对编码调制视觉诱发电位(c-VEP) BCI的目标识别性能的影响。...脑机接口(brain-computer interface,BCI)的分类性能一定程度上取决于对脑电信号的预处理方法,这项研究提出了一种空域时域滤波的预处理方法,以解决人类视觉系统中的潜伏延迟对编码调制视觉诱发电位(c-VEP) BCI的目标识别性能的影响。基于一个平均信号和单次试验信号之间的最小均方误差(the least mean square error,LMSE)创建时域空域滤波器,并且通过最小绝对收缩和选择算子(the least absolute shrinkage and selection operator,LASSO)将稀疏约束应用于滤波器的权重系数,并用模板匹配法来对目标进行识别。将算法应用于由63比特的M序列及其循环移位序列调制的16个目标的c-VEP BCI,并与通用的空域滤波算法典型相关分析(CCA)及空域时域逆滤波算法进行比较。结果表明本研究所提出的算法在分类准确率方面优于其他两种算法。展开更多
基金Central Government Funds for Guiding Local Science and Technology Development(No.ZY20198022)Graduate Education Innovation Program of Guangxi University of Traditional Chinese Medicine(No.YCXJ2021067)。
文摘Objective:To investigate the intervention of Xuduan Zhongzi Formula on the epididymis structure of model of oligospermia mice.Methods:Ten of the 45 male mice were labeled as the normal group,and the remaining 35 mice were injected with chloral hydrate for five consecutive days,and the randomly selected five mice were anesthetized with chloral hydrate.The left epididymis was removed,and a few sperm were found in the epididymis under the optical microscope,indicating successful construction of the model.They were randomly divided into three groups:the normal group,the L-carnitine group and the Xuduan group with 10 rats in each group.The the L-carnitine group and the Xuduan group were administrated with equivalent dose of human solvent,and the normal group and the model group were administrated with normal saline.After 8 weeks of intragastric administration,all experimental mice were killed,and the left epididymis was extracted for sperm detection,seminal plasma biochemical detection,HE staining,and electron microscopy.Results:In the model group,enlarged epididymal epithelial cells,vacuolar degeneration of primary and basal cells,and edema of interstitial cells and vascular dilation were found.Compared with the normal group,semen concentration,activity,SOD,A-glucosidase and fructose in the model group were significantly decreased,and the difference was statistically significant(P<0.01).After eight weeks of drug intragastric treatment,the semen concentration,activity,SOD,A-glucosidase and fructose in the Xuduan group were significantly increased compared with the model group,and the difference was statistically significant(P<0.01).Conclusion:Xuduan Zhongzi Formula can significantly improve the epididymal structure,the microenvironment of epididymal sperm maturation and the stability of epididymal epithelial structure of oligospermia model mice,creating conditions for sperm maturation in the epididymis.
文摘脑机接口(brain-computer interface,BCI)的分类性能一定程度上取决于对脑电信号的预处理方法,这项研究提出了一种空域时域滤波的预处理方法,以解决人类视觉系统中的潜伏延迟对编码调制视觉诱发电位(c-VEP) BCI的目标识别性能的影响。基于一个平均信号和单次试验信号之间的最小均方误差(the least mean square error,LMSE)创建时域空域滤波器,并且通过最小绝对收缩和选择算子(the least absolute shrinkage and selection operator,LASSO)将稀疏约束应用于滤波器的权重系数,并用模板匹配法来对目标进行识别。将算法应用于由63比特的M序列及其循环移位序列调制的16个目标的c-VEP BCI,并与通用的空域滤波算法典型相关分析(CCA)及空域时域逆滤波算法进行比较。结果表明本研究所提出的算法在分类准确率方面优于其他两种算法。
