目的建立高效液相色谱串联质谱法快速检测鸡蛋中硝基咪唑类药物残留量的分析方法。方法以0.1%甲酸乙腈为提取溶剂,加入盐析剂分层后,用Waters Oasis PRiME HLB法净化,用乙腈和0.1%(V:V)甲酸水溶液作为流动相进行梯度洗脱, C18色谱柱分离...目的建立高效液相色谱串联质谱法快速检测鸡蛋中硝基咪唑类药物残留量的分析方法。方法以0.1%甲酸乙腈为提取溶剂,加入盐析剂分层后,用Waters Oasis PRiME HLB法净化,用乙腈和0.1%(V:V)甲酸水溶液作为流动相进行梯度洗脱, C18色谱柱分离,在高效液相色谱-串联质谱仪上采用电喷雾正离子扫描模式检测,基质匹配标准曲线外标法定量。结果该方法在0.5~100 ng/mL范围内具有良好线性关系, 10种硝基咪唑类药物相关系数均大于(r^2)>0.99,方法检出限在0.1~0.3μg/kg范围,定量限在0.3~0.5μg/kg范围。3个浓度水平1.0、5.0、10.0μg/kg加标,平均回收率分别为82.5%~119.1%、80.7%~112.3%、80.6%~118.5%,相对标准偏差(relative standard deviation, RSD)为1.2%~9.1%。结论该方法具有快速、简便、准确、灵敏的特点,适用于鸡蛋中硝基咪唑残留量的分析检测,可用于日常大批量快速筛查工作,有效提高日常工作效率。展开更多
Chaetocin is a natural metabolite product with various biological activities and pharmacological functions isolated from Chaetomium species fungi belonging to the thiodiketopyrazines.Numerous studies have demonstrated...Chaetocin is a natural metabolite product with various biological activities and pharmacological functions isolated from Chaetomium species fungi belonging to the thiodiketopyrazines.Numerous studies have demonstrated a wide range of antitumor activities of chaetocin in vitro and in vivo.Several studies have demonstrated that chaetocin suppresses the growth and proliferation of various tumour cells by regulating multiple signalling pathways related to tumour initiation and progression,inducing cancer cell apoptosis(intrinsic and extrinsic),enhancing autophagy,inducing cell cycle arrest,as well as inhibiting tumour angiogenesis,invasion and migration.The antitumor effects and molecular mechanisms of chaetocin are reviewed and analysed in this paper,and the prospective applications of chaetocin in cancer prevention and therapy are also discussed.Our review provides the theoretical basis for exploiting the clinical application of chaetocin in cancer treatment.展开更多
文摘目的建立高效液相色谱串联质谱法快速检测鸡蛋中硝基咪唑类药物残留量的分析方法。方法以0.1%甲酸乙腈为提取溶剂,加入盐析剂分层后,用Waters Oasis PRiME HLB法净化,用乙腈和0.1%(V:V)甲酸水溶液作为流动相进行梯度洗脱, C18色谱柱分离,在高效液相色谱-串联质谱仪上采用电喷雾正离子扫描模式检测,基质匹配标准曲线外标法定量。结果该方法在0.5~100 ng/mL范围内具有良好线性关系, 10种硝基咪唑类药物相关系数均大于(r^2)>0.99,方法检出限在0.1~0.3μg/kg范围,定量限在0.3~0.5μg/kg范围。3个浓度水平1.0、5.0、10.0μg/kg加标,平均回收率分别为82.5%~119.1%、80.7%~112.3%、80.6%~118.5%,相对标准偏差(relative standard deviation, RSD)为1.2%~9.1%。结论该方法具有快速、简便、准确、灵敏的特点,适用于鸡蛋中硝基咪唑残留量的分析检测,可用于日常大批量快速筛查工作,有效提高日常工作效率。
文摘Chaetocin is a natural metabolite product with various biological activities and pharmacological functions isolated from Chaetomium species fungi belonging to the thiodiketopyrazines.Numerous studies have demonstrated a wide range of antitumor activities of chaetocin in vitro and in vivo.Several studies have demonstrated that chaetocin suppresses the growth and proliferation of various tumour cells by regulating multiple signalling pathways related to tumour initiation and progression,inducing cancer cell apoptosis(intrinsic and extrinsic),enhancing autophagy,inducing cell cycle arrest,as well as inhibiting tumour angiogenesis,invasion and migration.The antitumor effects and molecular mechanisms of chaetocin are reviewed and analysed in this paper,and the prospective applications of chaetocin in cancer prevention and therapy are also discussed.Our review provides the theoretical basis for exploiting the clinical application of chaetocin in cancer treatment.
