氮氧同位素示踪湿沉降NO_(3)^(-)来源及氧化途径

为探明焦作市大气湿沉降中硝态氮的污染水平,识别其来源及其形成过程,于2020年1月-2021年12月采集了焦作市大气湿沉降样品41个,测定并分析了TN、NH_(4)^(+)-N、NO_(3)^(-)-N浓度以及δ^(15)N-NO_(3)^(-)、δ^(18)O-NO_(3)^(-)值.结果表明:①TN、NH_(4)^(+)-N、NO_(3)^(-)-N浓度范围分别为2.52~13.27、0.11~1.70、1.64~8.31 mg/L,焦作市湿沉降中氮的主要存在形态为NO_(3)^(-)-N,占比为52.11%~83.92%.②δ^(18)O-NO_(3)^(-)、δ^(15)N-NO_(3)^(-)值的范围分别为54.9‰~93.9‰、-9.8‰~3.0‰,非雨季δ^(18)O-NO_(3)^(-)值(62.5‰~93.3‰)更接近于N2O5氧化途径生成的δ^(18)O-HNO_(3)值(100.9‰~103.1‰),雨季δ^(18)O-NO_(3)^(-)值(53.2‰~73.0‰)更接近于OH氧化途径生成的δ^(18)O-HNO_(3)值(50.9‰~60.9‰),说明非雨季大气湿沉降NO_(3)^(-)主要来自N2O5的氧化,而雨季主要来自OH的氧化.③雨季,OH和N2O5氧化途径对湿沉降NO_(3)^(-)的贡献率分别为80.32%和19.67%,非雨季,两种氧化途径的贡献率分别为65.37%和34.62%;在考虑了同位素分馏情况下,基于贝叶斯模型识别了湿沉降NO_(3)^(-)的主要来源,即土壤排放、生物质燃烧、机动车尾气排放和煤炭燃烧,其贡献率平均值分别为31.70%±4.90%、28.30%±14.00%、22.80%±12.10%、17.20%±0.08%.该研究结果将为降雨中硝酸盐的来源及形成途径提供证据.

Evaluation of facial temperature distribution changes during meditation using infrared thermal imaging:An experimental,cross-over study

Objective:To investigate the differences between meditation and resting states using infrared thermal imaging(IRTI)to determine facial temperature distribution features during meditation and annotate the patterns of facial temperature changes during meditation from the perspective of traditional Chinese medicine facial diagnosis.Methods:Each participant performed 10 min meditation and 10 min resting but in different sequences.A concentration test was set as the task load,followed by a meditation/resting or resting/meditation session,during which the participants'facial temperatures were observed using IRTI.Participants were scored on the Big Five Inventory(BFI)and Mindful Attention Awareness Scale(MAAS).Results:Forehead temperatures decreased more during meditation than during the resting state.The chin temperature increased only during meditation(P<.0001).For the subjects with meditation experience,there were significant differences in the temperatures of the left forehead(P<.01),right forehead(P<.01)and chin(P<.05)between the meditation and resting state at the 10~(th)min.In the nontask state,the BFI-Extraversion showed a negative correlation with the temperature of the left forehead(R=-0.41,P=.03).In the post-task state,the temperature of the left forehead was negatively correlated with scores on the MAAS(R=-0.42,P=.02).Conclusion:Using IRTI to study meditation offers a practical solution to the challenges in meditation research.The results indicate that an increase in chin temperature may be a representative feature of a meditation state,and forehead temperature is also a potential indicator.

老年人跌倒风险感知研究现状及其对老年人主动跌倒预防的启示

跌倒是老年人致病致死的重要原因。临床医护人员虽已重视对跌倒的管理,但跌倒干预依从性尚不理想,跌倒发生率仍然维持在较高水平,从老年人角度探讨其对跌倒风险的理解可能是跌倒干预,尤其是主动跌倒预防的关键。本文从“健康第一责任人”的角度,对老年人跌倒风险感知的概念、行为后果及影响因素进行总结,发现目前国内外跌倒风险感知研究较少,概念内涵仍需进一步挖掘。老年人对跌倒风险的主观认知能够导致不同的跌倒风险应对行为模式,从而影响跌倒风险。跌倒风险感知影响因素主要聚焦于情绪心理、生理状况、社会文化和物理环境方面,影响过程均通过主观认知发挥作用。未来可结合风险感知形成过程,使用实证研究进一步验证跌倒风险感知的心理认知机制,为主动跌倒预防提供实证依据。

力学性能可调的高氧钛原位电弧增材制造

通过电弧增材制造原位制备高氧钛,并对高氧钛的成分偏析、显微组织和力学性能进行研究。结果表明,高氧钛试样的显微组织主要呈现为针状α相集束。随着氧含量的增加,试样针状α相逐渐变宽,集束尺寸变大。受氧的强化作用以及晶粒尺寸效应共同影响,试样的强度先提高后下降,伸长率显著下降,断裂形式由塑性断裂向解理断裂过渡。当氧含量达到0.37%(质量分数)时,伸长率约为7%,试样抗拉强度超过730 MPa,约为商业纯钛的2倍。

Prevention of hepatocellular carcinoma in chronic viral hepatitis B and C infection

Hepatocellular carcinoma(HCC)is a leading cause of cancer-related mortality worldwide,with the majority of cases associated with persistent infection from hepatitis B virus(HBV)or hepatitis C virus(HCV).Natural history studies have identified risk factors associated with HCC development among chronic HBV and HCV infection.High-risk infected individuals can now be identified by the usage of risk predictive scores.Vaccination plays a central role in the prevention of HBV-related HCC.Treatment of chronic HBV infection,especially by nucleoside analogue therapy,could also reduce the risk of HBV-related HCC.Concerning HCV infection,besides the advocation of universal precautions to reduce the rate of infection,pegylated interferon and ribavirin could also reduce the risk of HCV-related HCC among those achieving a sustained virologic response.Recently there has been mounting evidence on the role of chemopreventive agents in reducing HBV-and HCV-related HCC.The continued advances in the understanding of the molecular pathogenesis of HCC would hold promise in preventing this highly lethal cancer.

Efficient decolorization of dye-containing wastewater using mycelial pellets formed of marine-derived Aspergillus niger

In order to improve the efficient decolorization of dye-containing water by biosorbent and understand the biosorption mechanism, the self-immobilization mycelial pellets were prepared using a marine-derived fungus Aspergillus niger ZJUBE-1, and an azo dye, Congo red was chosen as a model dye to investigate batch decolorization efficiency by pellets. The pellets as biosorbent showed strong salt and acid tolerance in biosorption process. The results for dye adsorption showed that the biosorption process fitted well with models of pseudo-second-order kinetic and Langmuir isotherm, with a maximum adsorption capacity of 263.2 mg·g^(-1) mycelium. During 6 batches of continuous decolorization operation, the mycelial pellets could possess efficient decolorization abilities(>98.5%).The appearance of new peak in the UV–Vis spectral result indicated that the decolorization process may also contain biodegradation. The mechanism studies showed that efficient biosorption ability of pellets only relies on the active zone on the surface of the pellet, which can be enhanced by nutrition supplement or be shifted outward by a reculture process.

Neuroprotection of phenolic alkaloids from Menispermum dauricum versus melatonin against oxygen-glucose-serum-deprivation-mediated injury

BACKGROUND： Recent studies have demonstrated that phenolic alkaloids from Menispermum dauricum （PAMD） can protect the heart and brain from ischemia/reperfusion injury, and promote neuron survival by inhibiting neuronal Bax and upregulating Bcl-2 expression following ischemia/reperfusion. OBJECTIVE： To investigate the neuroprotective effects of PAMD versus exogenous melatonin against ischemia/reperfusion injury. DESIGN, TIME AND SETTING： Observation and comparison experiments at a cellular level were performed at the Department of Biochemistry and Molecular Biology, Tongji Medical College of Huazhong University of Science and Technology between February 2007 and February 2008. MATERIALS： PAMD （95% purity） was provided by Kunming Institute of Botany, Chinese Academy of Sciences; melatonin was provided by Sigma, USA. METHODS： N2a mouse neuroblastoma cells were cultured in vitro deprived of glucose, serum and oxygen for 90 minutes, then cultured in normal medium containing different concentrations of PAMD （0.1, 1.0, 10 mg/L） or melatonin （1, 10, and 100 μmol/L）. Cells cultured in normal conditions served as a control. MAIN OUTCOME MEASURES： The culture solution was collected to determine the content of ex- citatory neurotransmitters such as glutamic acid and aspartic acid; cell viability was detected by MTT methods; reactive oxygen species production was determined by fluorescence spectroscopy; mito- chondrial transmembrane potential （？Ψm） was detected by laser confocal scanning; cytochrome C was measured by western blotting; and caspase-3 activity was determined by visible spectropho- tometry. RESULTS： Melatonin and PAMD both promoted oxygen-glucose-serum deprivation-mediated N2a cell survival （P 〈 0.01） and inhibited glutamic acid release （P 〈 0.01）, but melatonin did not inhibit aspartic acid production. The protective effects were the strongest using melatonin 100 μmol/L and PAMD 10 mg/L, so subsequent experiments were the performed at those doses. Although PAMD could no longer maintain mitochondrial transmembrane potential 6 hours after reperfusion, its in- hibitory effects on cytochrome C release from mitochondria and scavengers of reactive oxygen species were stronger than those of melatonin （P 〈 0.01）. However, its inhibitory effect on caspase-3 activity was weaker than that of melatonin： PAMD could inhibit caspase-3 activity 12 hours after reperfusion （P 〈 0.01）, but melatonin inhibited caspase-3 activity 28 hours after reperfusion （P 〈 0.01）. CONCLUSION： The results show that melatonin and PAMD have neuroprotective effects, but that the mechanisms are varied. Melatonin can maintain mitochondrial transmembrane potential, but its inhibitory effects on cytochrome C release, caspase-3 activity, and reactive oxygen species scav-enging are different from those of PAMD.

Salt-tolerant mechanism of marine Aspergillus niger cellulase cocktail and improvement of its activity

The cellulase cocktail produced by marine Aspergillus niger exhibits a property of salt-tolerance,which is of great potential in cellulose degradation in high salt environment.In order to explain the mechanism on the salttolerance of the cellulase cocktail produced by marine A.niger,six cellulase components(AnCel6,AnCel7A,AnCel7B,AnEGL,AnBGL1 and AnBGL2)were obtained by directed expression.Studies on their enzymatic properties revealed that oneβ-glucosidase(AnBGL2)and one endoglucanase(AnEGL)exhibited an outstanding salttolerant property,and one cellobiohydrolase(AnCel7B)exhibited a certain salt-tolerant property.Subsequent study revealed that the salt-tolerant An EGL and AnCel7B endowed the cellulase cocktail with stronger salttolerant property,while the salt-tolerant An BGL2 had no positive effect.Moreover,after overexpression of AnCel6,AnCel7A,AnCel7B and AnEGL,the activity of cellulase cocktail increased by 80%,70%,63%and 68%,respectively.However,the activity of cellulase cocktail was not improved after overexpression of AnBGL1 and AnBGL2.After mixed-strain fermentation with cellobiohydrolase recombinants(cel6 a,cel7a and cel7b recombinants)and endoglucanase recombinant(egl recombinant),the the activity of cellulase cocktail increased by 114%,102%and91%,respectively.

Removal process and mechanism of hexavalent chromium by adsorption-coupled reduction with marine-derived Aspergillus niger mycelial pellets

In order to remove hexavalent chromium(Cr(Ⅵ))from solutions efficiently,the mycelial pellets with a marine-derived fungus Aspergillus niger as a biosorbent were prepared.The effects of removal process parameters such as solution pH,initial Cr(Ⅵ)concentration and biomass concentration on Cr(Ⅵ)removal process were investigated.The results showed that Cr(Ⅵ)removal rate up to 100%could be achieved under optimized conditions,which indicated the excellent Cr(Ⅵ)removal performance of the Aspergillus niger pellets.As a more important point,the Cr(Ⅵ)removal mechanism was studied,and the results revealed that Cr(Ⅵ)removal was achieved in the adsorption-coupled reduction process.A little of Cr(Ⅵ)was reduced to less toxic trivalent chromium(Cr(Ⅲ))in solution,while some was absorbed on the surface of mycelial pellets.Then they may be reduced on the surface or transferred into cells and then be reduced.The marine-derived A.niger mycelial pellets show properties of easy preparation and separation and cost effectiveness,which are potential biosorbent and reductant in the treatment of trace chromate containing wastewater.

原花青素在深龋的治疗及修复中的应用进展

原花青素(proanthocyanidin,PA)是一种天然植物类多酚。由于PA具有促进再矿化、诱导胶原交联、抑制蛋白酶活性、抗菌等多种生物学作用,使其在治疗及修复深龋的临床应用中有着广阔的应用前景,例如促进牙本质再矿化、改善树脂-牙本质粘接效果、改善牙本质酸蚀效果等。首先,PA不仅能通过其自身或与其他再矿化剂联合促进牙本质再矿化,同时它还具有抗菌作用,在减少致龋病原菌及其生物膜形成的同时能够抑制其产酸,基于以上特性,PA能够减少龋病的发生,进而改善深龋治疗后的远期效果。此外,将PA加入到粘接剂或酸蚀剂中,利用其诱导胶原交联、抑制蛋白酶活性等生物学作用,能够改善对牙本质的酸蚀及粘接效果,继而达到改善深龋修复效果的最终目的。本文归纳总结了近年来PA在深龋的治疗及修复等方面的相关研究进展,从促进牙本质再矿化、抗菌和改善牙本质的粘接及酸蚀效果四个方面进行综述,以期能为临床深龋的治疗及修复提供更全面的参考。

Solution combustion synthesis of high-entropy metal spinel oxide as oxygen evolution reaction catalyst

High-entropy metal spinel oxide(HEO)is proved to be a promising oxygen evolution reaction(OER)catalyst with high catalytic performance and stability.A short routine synthesis process based on solution combustion synthesis was proposed to prepare(Co_(0.25)Ni_(0.25)Mn_(0.25)Zn_(0.25))Fe_(2)O_(4)spinel HEO in this work.During the process,the products were rapidly synthesized and melted due to the high-temperature reaction,and then quickly crystallized on the surface of nickel foam to form a nano-structure coating.With the aid of nano-scale spinel structure,the overpotential of non-activation HEO electrode reaches 276 mV at the current density of 10 mA·cm^(-2),and after 100 h i-t test,it can be further reduced to 230 mV,which proves the high activity of OER catalysis.The promotion of OER catalytic performance can be attributed to the surface reconstruction caused by the selective element leaching and the boost of oxygen vacancy,leading to the formation of nano-scale flocculation around spinel core after the long-term OER process.This work indicates a special casting process for functional materials and explores the application of rapid crystallization.

2022年版《世界指南:老年人跌倒的预防与管理》解读

随着我国迈入深度老龄化社会,老年人跌倒和跌倒相关伤害问题受到广泛关注,国内外相关研究和指南不断更新。来自39个国家、96位专家组成的世界跌倒预防指南小组于2022年制定了《世界指南:老年人跌倒的预防与管理》。该指南着眼于“以人为本”的理念,充分考虑老年人的跌倒管理需求及照顾者和其他利益相关方对跌倒的看法,并考虑指南在各照护环境和资源有限地区的适用性,结合应用电子健康进行跌倒预防的前沿证据,针对社区老年人的跌倒风险初筛、跌倒风险评估、跌倒风险分级管理提供新的实践指导建议。本文将深入解读该指南,提取该指南中行之有效的实践指导建议进行分析,为我国的医护工作者和社区卫生工作者预防和管理老年人跌倒提供参考。

Preparation, properties, and cell attachment/growth behavior of chitosan/acellular derm matrix composite materials

Composite membranes and sponge scaffolds consisting chitosan (CS) and acellular derm matrix (ADM) in six ratios were prepared by solvent evaporation technique and freeze-drying method, respectively. The composite materials were characterized by water contact angle measurement, scanning electron microscopy (SEM), water absorption and HaCat cells compatibility. The SEM result showed that CS/ADM three-dimensional (3D) micro-porous structures were successfully produced. The water absorption value of all scaffolds was over 18 times of its initial weight, which is high enough for skin regeneration scaffold, but there were no significant differences of water absorption ratio between deionized water and PBS solution for same scaffold (P > 0.05). HaCat cells were distributed uniformly on the surfaces of membrane 4-6, and an almost confluent monolayer was formed on membrane 6 on the fifth day, whereas cells maintained round and spherical in shape on the surface of membrane 1. The results showed that the cell compatibility of pure CS membrane needed to be improved, and addition of ADM realized this purpose. The results of compatibility of HaCat cells on scaffolds showed that the cell proliferated well on the scaffolds 3 and 4. In our study, the cell’s attachment and growth on the composite membranes was mainly determined by the content of the membrane, whereas the cell’s attachment and growth in the scaffolds was determined by both the content and structure of the scaffolds.

人工智能算法偏见与健康不公平的成因与对策分析

随着信息技术的发展,人工智能为疾病诊疗带来重要价值。然而,人工智能中存在算法偏见现象,可导致医疗卫生资源分配不均等问题,严重损害患者的健康公平。算法偏见是人为偏见的技术化体现,其形成与人工智能开发过程密切相关,主要源于数据收集、训练优化和输出应用3个方面。医护工作者作为患者健康的直接参与者,应采取相应措施以预防算法偏见,避免其引发健康公平问题。医护工作者需保障健康数据真实无偏见、优化人工智能的公平性和加强其输出应用的透明度,同时需思考如何处理临床实践中算法偏见引发的不公平现象,全面保障患者健康公平。本研究就健康领域中算法偏见的形成原因和应对策略展开综述,以期提高医护工作者识别和处理算法偏见的意识与能力,为保障信息化时代中的患者健康公平提供参考。

社区老年人自评健康与衰弱的关联:淡漠的调节作用

背景既往研究表明,自评健康状况能够作为衰弱的简易评估指标,自评健康状况差的个体衰弱风险更高。然而在淡漠老年人群中,自评健康状况与衰弱间的关联强度可能不同,健康状况自我评价法在衰弱评估中的应用效果可能存在差异。目的探讨淡漠在社区老年人自评健康状况与衰弱关联中的调节作用,旨在为健康状况自我评价法在淡漠老年人衰弱评估中的应用提供理论指导。方法2021年11月—2022年3月,采用便利抽样法,抽取南京市和连云港市社区老年人共384名作为调查对象(其中179名来自南京市江宁区东山街道东山社区,205名来自连云港市东海县青湖社区),采用一般资料调查表、衰弱评估量表(FFP)、老年人抑郁量表淡漠分量表(GDS-3)、自评健康条目对其进行问卷调查。采用广义线性模型分析社区老年人自评健康状况对衰弱的影响,采用Hayes编制的SPSS宏程序Process的模型1分析淡漠在社区老年人自评健康状况和衰弱关系中的调节作用,并进行简单斜率分析、绘制简单斜率图。结果384名社区老年人中位FFP、自评健康条目得分分别为1.00(2.00)、4.00(1.00)分,淡漠检出率为55.5%(213/384)。广义线性模型结果显示,社区老年人自评健康状况对衰弱有影响(b=0.310,P<0.001)。调节效应检验结果显示,淡漠在社区老年人自评健康状况和衰弱的关系中起调节作用(b=0.355,t=3.074,P=0.002)。简单斜率分析结果显示,非淡漠组的简单斜率为0.100(t=1.209,P=0.228),淡漠组的简单斜率为0.455(t=5.206,P<0.001)。结论社区老年人自评健康状况与衰弱之间存在关联,健康状况自我评价法的应用可以帮助社区医务人员评估老年人的衰弱情况。淡漠在社区老年人自评健康状况和衰弱的关系中发挥调节作用,相较于非淡漠老年人,淡漠老年人自评健康状况与衰弱之间存在明显的关联,在淡漠老年人中加强其对自身健康状况的自我评价利于其衰弱的识别。

衰弱在社区老年人淡漠与跌倒风险的中介效应研究

背景淡漠老年人跌倒风险高且易重复跌倒,改善淡漠虽有助于减少老年人的跌倒风险,但淡漠的干预效果不佳,而改善衰弱可为减少淡漠老年人跌倒风险提供新方法。目的探讨衰弱在社区老年人淡漠与跌倒风险间的中介效应,为干预淡漠老年人跌倒提供新思路。方法2021年11月至2022年3月采用便利抽样法抽取南京市和连云港市社区老年人共212例,其中南京市东山社区卫生服务中心128例,连云港市东海县青湖镇社区84例。采用一般资料调查表,衰弱评估量表(FFP,评估衰弱情况),老年人抑郁量表(GDS-3,评估淡漠),预防老年人意外、死亡、伤害工具包(STEADI,评估跌倒风险)进行问卷调查。分析衰弱在淡漠与跌倒风险中的中介作用。结果共发放问卷212份,回收有效问卷192份,有效回收率为90.6%。192例社区老年人中跌倒风险总分为2.0(0,4.0)分,淡漠总分为(1.6±0.9)分,衰弱总分为0(0,2.0)分。无跌倒风险136例(70.8%),有跌倒风险56例(29.2%)。Spearman秩相关分析结果显示,淡漠与衰弱、跌倒风险呈正相关(rs值分别为0.303、0.388,P<0.05);衰弱与跌倒风险呈正相关(rs=0.424,P<0.05)。Model 4中介模型结果显示,淡漠对跌倒风险的正向影响作用显著(B=1.011,t=5.207,P<0.05),淡漠对衰弱的正向影响作用显著(B=0.324,t=3.800,P<0.05),衰弱对跌倒风险的正向影响作用显著(B=0.679,t=4.173,P<0.05)。Bootstrap检验模型分析结果显示,“淡漠→衰弱→跌倒风险”路径中衰弱效应值0.22[95%CI(0.08,0.40)],中介效应成立。淡漠可以直接影响跌倒风险以外,还可以间接通过衰弱影响跌倒风险。总效应为1.01,直接效应为0.79,中介效应为0.22,中介效应值占总效应的21.78%。结论衰弱是社区老年人淡漠与跌倒风险之间的中介变量,改善老年人衰弱是减少淡漠老年人跌倒风险的重要途径。

HSP90AA1 promotes lymphatic metastasis of hypopharyngeal squamous cell carcinoma by regulating epithelial-mesenchymal transition

Lymphatic metastasis(LM)emerges as an independent prognostic marker for hypopharyngeal squamous cell carcinoma(HSPSCC),chiefly contributing to treatment inefficacy.This study aimed to scrutinize the prognostic relevance of HSP90AA1 and its potential regulatory mechanism of concerning LM in HPSCC.Methods:In a preceding investigation,HSP90AA1,a differential gene,was discovered through transcriptome sequencing of HPSCC tissues,considering both the presence and absence of LM.Validation of HSP90AA1 expression was accomplished via qRT-PCR,western-blotting(WB),and immunohistochemistry(IHC),while its prognostic significance was assessed employing Kaplan–Meier survival analysis(KMSA),log-rank test(LR),and Cox’s regression analysis(CRA).Bioinformatics techniques facilitated the prediction and analysis of its plausible mechanisms in LM,further substantiated by in vitro and in vivo experiments utilizing FaDu cell lines.Results:HSP90AA1 is substantially upregulated in HPSCC with LM and is identified as an independent prognostic risk determinant.The down-regulation of HSP90AA1 can achieve inhibition of tumor cell proliferation,migration and invasion.Both in vivo experiments and Bioinformatics exploration hint at promoting LM by Epithelial-mesenchymal transition(EMT),regulated by HSP90AA1.Conclusions:HSP90AA1,by controlling EMT,can foster LM in HPSCC.This finding sets the foundation for delving into new therapeutic targets for HPSCC.

液相色谱-串联质谱测定洋葱中的五种前列腺素

该研究建立了科学、准确、高效的液相色谱-串联质谱分析检测洋葱中5种前列腺素(prostaglandins,PG)的方法。样品经乙醇提取后,用无水硫酸镁吸收水分,在高速冷冻离心机上离心后在旋转蒸发仪上蒸发近干,最后用甲醇+乙腈复溶,5 mmol/L甲酸铵溶液+乙腈+甲醇为流动相,梯度洗脱,流速为0.2 mL/min,柱温为35℃,进样量为2μL,提取物经色谱柱分离,在电喷雾负离子模式下,采用四级杆质谱仪进行多反应分析。在实验浓度范围内,5种PG标准曲线线性良好,相关系数(R^(2))均大于0.999;在10、20、40 ng/mL添加浓度水平,所得5种PG的回收率为96%~109%,相对标准偏差为0.26%~1.81%,检出限和定量限分别为1.0、2.5μg/kg。对样品及样品加标后分别采用该方法和文献报道方法提取分析,通过试验数据对比表明该方法的提取效果更好,并且加标回收率远高于文献报道方法,进一步证明了该方法的可行性。结果表明,该法科学、准确、操作简便,完全满足洋葱中5种PG的分析检测要求。

miR-125b-2-3p表达对鼻咽癌细胞HNE1增殖和凋亡的影响

目的探讨miR-125b-2-3p表达对鼻咽癌细胞HNE1增殖和凋亡的影响。方法荧光定量PCR检测miR-125b-2-3p在人永生化鼻咽上皮细胞NP69和鼻咽癌细胞中的表达。荧光定量PCR检测上调和下调miR-125b-2-3p表达转染效率。实验分为control组(未处理)、mimics NC组(Lip 2000+mimics NC)、miR-125b-2-3p mimics组(Lip 2000+miR-125b-2-3p mimics)、inhibitor NC组(Lip 2000+inhibitor NC)和miR-125b-2-3p inhibitor组(Lip 2000+miR-125b-2-3p inhibitor)。CCK-8实验、细胞集落形成实验和活细胞/死细胞双染色实验检测细胞增殖的情况;流式细胞术检测细胞凋亡情况;DAPI细胞核染色检测细胞核的形态变化;线粒体膜电位检测细胞早期凋亡情况。结果与人永生化鼻咽上皮细胞NP69相比,鼻咽癌细胞中miR-125b-2-3p的表达较高(P<0.05)。与control组和mimics NC组相比,miR-125b-2-3p mimics组miR-125b-2-3p表达上调(P<0.01);与control组和inhibitor NC组相比,miR-125b-2-3p inhibitor组miR-125b-2-3p表达下调(P<0.01)。与mimics NC组相比,miR-125b-2-3p mimics组细胞增殖能力较强(P<0.01),细胞凋亡能力较弱(P<0.01),线粒体膜电位较高(P<0.01);相较于inhibitor NC组,miR-125b-2-3p inhibitor组细胞增殖能力被抑制(P<0.01),细胞凋亡能力较强(P<0.01),核固缩核碎裂较多,线粒体膜电位较低(P<0.05)。结论下调miR-125b-2-3p能抑制鼻咽癌细胞HNE1的增殖,促进细胞的凋亡。

Eff ects of early standardized enteral nutrition on preventing acute muscle loss in the acute exacerbation of chronic obstructive pulmonary disease patients with mechanical ventilation

BACKGROUND:To investigate the eff ects of early standardized enteral nutrition(EN)on the crosssectional area of erector spine muscle(ESMcsa),plasma growth diff erentiation factor-15(GDF-15),and 28-day mortality of acute exacerbation of chronic obstructive pulmonary disease(AECOPD)patients with invasive mechanical ventilation(MV).METHODS:A total of 97 AECOPD patients with invasive MV were screened in the ICUs of the First People's Hospital of Lianyungang.The conventional EN group(stage Ⅰ)and early standardized EN group(stage Ⅱ)included 46 and 51 patients,respectively.ESMcsa loss and GDF-15 levels on days 1 and 7 of ICU admission and 28-day survival rates were analyzed.RESULTS:On day 7,the ESMcsa of the early standardized EN group was significantly higher than that of the conventional EN group,while the plasma GDF-15 levels were significantly lower than those in the conventional EN group(ESMcsa:28.426±6.130 cm^(2) vs.25.205±6.127 cm^(2);GDF-15:1661.608±558.820 pg/mL vs.2541.000±634.845 pg/mL;all P<0.001).The 28-day survival rates of the patients in the early standardized EN group and conventional EN group were 80.40%and 73.90%,respectively(P=0.406).CONCLUSION:ESMcsa loss in AECOPD patients with MV was correlated with GDF-15 levels,both of which indicated acute muscular atrophy and skeletal muscle dysfunction.Early standardized EN may prevent acute muscle loss and intensive care unit-acquired weakness(ICU-AW)in AECOPD patients.