尾矿库环境事故因素分析及建议

基于我国尾矿库环境风险特征,分析近年来尾矿库环境事故原因,并针对环境事故发生后的应急处理及环境次生灾害应急处理展开讨论。针对我国尾矿资源存量大、类型多,资源综合利用率较低以及矿山企业生产管理水平不高的实际问题,提出建议:一是要实现尾矿资源的综合利用,达到减量减排的目的;二是要对尾矿库采取系统性的污染治理,特别是针对因不可抗拒因素而引起的环境污染问题;三是要加强安全管理,减少溃坝等安全风险,从源头降低安全事故次生环境污染风险。

Camellia sinensis CsMYB4a participates in regulation of stamen growth by interaction with auxin signaling transduction repressor CsAUX/IAA4

Subgroup 4(Sg4)members of the R2R3-MYB are generally known as negative regulators of the phenylpropanoid pathway in plants.Our previous research showed that a R2R3-MYB Sg4 member from Camellia sinensis(CsMYB4a)inhibits expression of some genes in the phenylpropanoid pathway,but its physiological function in the tea plant remained unknown.Here,CsMYB4a was found to be highly expressed in anther and filaments,and participated in regulating filament growth.Transcriptome analysis and exogenous auxin treatment showed that the target of CsMYB4a might be the auxin signal pathway.Auxin/indole-3-acetic acid 4(AUX/IAA4),a repressor in auxin signal transduction,was detected from a yeast two-hybrid screen using CsMYB4a as bait.Gene silencing assays showed that both CsIAA4 and CsMYB4a regulate filament growth.Tobacco plants overexpressing CsIAA4 were insensitive to exogenous a-NAA,consistent with overexpression of CsMYB4a.Protein-protein interaction experiments revealed that CsMYB4a interacts with N-terminal of CsIAA4 to prevent CsIAA4 degradation.Knock out of the endogenous NtIAA4 gene,a CsIAA4 homolog,in tobacco alleviated filament growth inhibition and a-NAA insensitivity in plants overexpressing CsMYB4a.All results strongly suggest that CsMYB4a works synergistically with CsIAA4 and participates in regulation of the auxin pathway in stamen.

中华民族现代文明内蕴的全人类共同价值及世界意义

中华民族现代文明是在“两个结合”,特别是“第二个结合”的过程中造就的新的文化生命体,它是中国式现代化创造的人类文明新形态,展现了开阔的世界意识。建设中华民族现代文明作为新的文化使命,突出彰显了“和平、发展”的共存道路定向,蕴含着“公平、正义”的邦国关系准则,折射出“民主、自由”的理想价值追求,为全人类共同价值提供了丰富的文化和思想滋养。中华民族现代文明破除了困扰人类文明发展的“西方中心论”“历史终结论”“文明冲突论”,为站在历史十字路口的人类应当何去何从提供了中国答案,集中彰显了中国共产党的世界担当,创新了人类文明共同体治理思路,在文明的交流互鉴中为人类文明发展进步作出原创性贡献。

基于最大功率点跟踪下垂控制的光储一体化系统研究

光储一体化系统相较于光储独立式系统具有更好的体积成本优势和分布式发电消纳能力,但现有一体化系统往往存在电能利用不充分的问题。为研究高功率密度、高光伏出力利用率的光储一体化设备,探究光储一体化系统稳定、可靠、高利用率的并网控制方法,本文对光储一体化系统内部重要元器件进行物理建模,分析光储一体化系统有功出力特性,提出一种基于最大功率点跟踪(MPPT)下垂控制的光储一体化系统控制方案,并在仿真软件中验证了方案的有效性。

CTRP6与妊娠期糖尿病患者糖脂代谢功能的相关性

目的分析C1q/肿瘤坏死因子相关蛋白6(CTRP6)与妊娠期糖尿病(GDM)患者糖脂代谢功能的相关性。方法选取50例GDM患者列为病例组,另选择同期孕检的66例正常产妇列为对照组,比较两组的一般资料(年龄、孕周、体质量指数、产次),临床资料(血糖指标、血脂指标、胰岛素功能、CTRP6表达),采用单因素分析、Logistic多因素回归分析归纳可导致GDM发病的危险因素,经Spearman相关性系数验证CTRP6表达与GDM患者糖脂代谢指标及胰岛素功能的相关性。结果Logistic多因素回归分析结果显示,空腹血糖(FBG)≥10.0 mmol/L、糖化血红蛋白(HbA1c)≥10%、甘油三酯(TG)≥2.5 mmol/L、低密度脂蛋白胆固醇(LDL-C)≥2.0 mmol/L、载脂蛋白A5(ApoA5)≤100μg/L、CTRP6≥600μg/L、空腹胰岛素(FINS)≥10 mU/L、胰岛β细胞功能(HOMA-β)≤50、胰岛素抵抗(HOMA-IR)≥2.25为发生GMD的危险因素。经Spearman相关性系数检验,CTRP6表达水平与FBG、HbA1c、TG、LDL-C、FINS、HOMA-IR正相关,与ApoA5、HOMA-β负相关。结论CTRP6表达水平随FBG、HbA1c、TG、LDL-C、FINS、HOMA-IR升高,随ApoA5、HOMA-β降低而不断上升。

General analytical method of designing shielded coils for arbitrary axial magnetic field

Magnetic coils for specific requirements are widely used in modern quantum physics. In this study, a general analytical method of designing the shielded coils for generating an arbitrary axial magnetic field is proposed. The theoretical formula for an axial magnetic field generated by a single shielded coil is obtained and used to construct specific coils. The structural parameters of these coils are determined by fitting the theoretical formula with their specific requirements. The feasibility of this method is proved by realizing four concrete kinds of coils: uniform magnetic field generating coils, gradient magnetic field generating coils, asymmetrical uniform magnetic field generating coils, and parabolic magnetic field generating coils. The correctness of these theoretical results is demonstrated by both the finite element simulations and the relevant experimental results. Furthermore, the application of this method is of great significance for developing the quantum physics and quantum devices in future.

瑞芬太尼联合咪达唑仑对ICU气管插管患者的镇痛镇静效果

目的探讨瑞芬太尼联合咪达唑仑对ICU气管插管患者的镇痛镇静效果。方法选取2019年1月至2021年12月于南昌大学第二附属医院接受气管插管的88例重症患者作为研究对象,采用随机数字表法分为研究组与对照组,每组44例。研究组给予瑞芬太尼联合咪达唑仑镇痛镇静,对照组给予瑞芬太尼镇痛镇静,比较两组给药前后不同时间点收缩压(SBP)、血氧饱和度(SaO_(2))、数字疼痛量表(NRS)评分、呼吸频率及镇静效果。结果两组呼吸频率、SBP、SaO_(2)组间、时间、交互比较差异有统计学意义(P<0.05)。组内比较:研究组给药后15 min(T1)、给药后30 min(T2)时SBP均低于T0,T2时呼吸频率低于T0、T1,差异有统计学意义(P<0.05),其他时点各指标两两比较差异无统计学意义;对照组T1、T2时呼吸频率、SBP、SaO_(2)均低于T0,且T2时SBP、SaO_(2)均低于T1,差异有统计学意义(P<0.05),其他时点各指标两两比较差异无统计学意义。组间比较:T0时,两组呼吸频率、SBP、SaO_(2)比较差异无统计学意义;T1时,研究组SaO_(2)高于对照组,差异有统计学意义(P<0.05),两组呼吸频率、SBP比较差异无统计学意义;T2时,研究组呼吸频率、SBP、SaO_(2)均高于对照组,差异有统计学意义(P<0.05)。两组NRS评分组间、时间、交互比较差异有统计学意义(P<0.05);两组给药后各时间点NRS评分均低于前一时间点,且研究组低于对照组,差异有统计学意义(P<0.05)。研究组镇静效果优于对照组,差异有统计学意义(P<0.05)。结论瑞芬太尼联合咪达唑仑镇静镇痛可有效维持ICU插管患者插管期间生命体征稳定,提高镇痛和镇静效果。

我国冬季项目优秀运动员身体形态和年龄特征分析

身体形态和年龄与运动员的竞技能力和运动表现有密切联系。对17支冬季项目国家队的538名运动员进行测试,分析运动员的身体形态(身高、体重、BMI、克托莱指数)和年龄特征,比较不同运动项目之间运动员在身体形态和年龄上的差异。结果表明:(1)我国冬季项目运动员在身体形态和年龄上存在显著性别差异;(2)冰上项目和雪上项目运动员在身体形态和年龄上均表现了显著差异;(3)不同冰上项目和雪上项目之间运动员在身体形态和年龄上也表现了差异。研究认为:我国冬季项目在运动选材和运动训练中要综合考虑身体形态指标的专项特点和年龄因素对运动员竞技能力的影响效应,提高选材的科学性和针对性。

多模式运动对轻度认知功能障碍老年人认知功能、神经营养因子及全脑体积的影响

目的:探索多模式运动对轻度认知功能障碍(mild cognitive impairment,MCI)老年人认知功能、神经营养因子(neurotrophic factors,NFs)及全脑体积的影响。方法:26名MCI老年人参与实验,随机分为对照组[n=14,年龄(74.77±6.04)岁]与运动组[n=12,年龄(73.83±7.51)岁],运动组进行20周多模式运动干预(60 min/次,3次/周)。评估干预前后认知功能、全脑体积及外周血清NFs水平。配对比较干预前后各类数据变化,并对认知功能变化与NFs、全脑体积变化关系进行相关分析。结果:运动干预后MCI老年人整体认知功能、注意力、言语流畅性显著提高(P<0.05),逻辑记忆与执行功能无明显变化(P>0.05);外周血清NFs水平升高、脑灰质体积增大、脑脊液体积缩小、脑组织分数占比提高(P<0.05);注意力与言语流畅性变化与脑灰质体积、脑组织分数,外周血清NFs水平的变化相关。结论:多模式运动干预可改善MCI老年人整体认知功能、注意力与言语流畅性,提高血清NFs水平,引起脑灰质体积增大,脑脊液体积缩小,脑组织分数占比提高;NFs在运动引起MCI老年人认知功能与脑结构改善上可能发挥中介作用。

辐流式二沉池的流场仿真及评价指标的构建

为了定量分析周进周出辐流式二沉池的内部流场结构、优化污泥絮体沉淀的水力条件,采用计算流体力学(Computational Fluid Dynamics,CFD)的方法对其进行数学建模及流场分析,创新提出了相应评价指标,包括:配水均匀性、流程池径比及沉淀时间比率。研究结果表明:利用流体体积(VOF)模型及k-ωSST湍流模型搭建的CFD模型适用于评估周进周出辐流式二沉池进水廊道配水均匀性;利用单相流模型及k-ωSST湍流模型搭建的CFD模型适用于评估池内流程池径比及沉淀时间比率;综合使用CFD模拟分析与经验公式设计优化配置导流装置调控流场,其配水均匀性、流程池径比及沉淀时间比率分别提升了20.0%,35.4%和39.0%。

正常睡眠—觉醒节律被干预的再生障碍性贫血小鼠外周血细胞数量、MT、NE水平变化观察

目的观察正常睡眠—觉醒节律被干预的再生障碍性贫血小鼠外周血细胞数量、昼夜节律调节因子(MT、NE)水平变化,以探讨睡眠—觉醒节律干预对再生障碍性贫血小鼠血细胞的影响及可能的作用机制。方法C57BL/6雄性小鼠随机分为实验组[睡眠—觉醒节律干预组(睡眠干预组、觉醒干预组、睡眠—觉醒干预组)和睡眠—觉醒节律未干预组]和正常对照组,实验组建立再生障碍性贫血小鼠模型,正常对照组未建立再生障碍性贫血小鼠模型。睡眠—觉醒节律未干预组及正常对照组保持正常睡眠—觉醒节律,睡眠干预组、觉醒干预组分别给予光照24 h、睡眠24 h干预,睡眠—觉醒干预组前3 d日间睡眠夜间觉醒,后3 d相反。各组共干预9天,在干预前1天和干预后第6天分别测量各组小鼠外周血细胞数。干预前采用ELISA法检测实验组和对照组小鼠血浆和骨髓中MT、NE、OX、PGE2。干预前1天和干预后3、6、9天分别测量各组小鼠血浆MT、NE。结果(1)各组干预前后血细胞比较:干预前,与正常对照组比较,实验组WBC、RBC、PLT数量及HGB水平低(P均<0.01);睡眠—觉醒节律干预第6天,与正常对照组相比,睡眠—觉醒节律未干预组、睡眠干预组WBC、RBC数量及HGB水平均低(P均<0.01);觉醒干预组及睡眠—觉醒干预组WBC数量、HGB水平低,PLT数量高(P均<0.01)。与睡眠—觉醒节律未干预组比较,觉醒干预组及睡眠—觉醒干预组RBC数量、HGB水平高(P均<0.01)。与睡眠干预组比较,觉醒干预组和睡眠—觉醒干预组PLT数量高(P均<0.05)。(2)各组被干预前后血浆MT及NE水平比较:被干预第3天:与正常对照组比较,睡眠—觉醒节律未干预组、觉醒干预组血浆MT水平高(P<0.01);与睡眠—觉醒节律未干预组比较,觉醒干预组血浆MT水平高(P<0.01),睡眠—觉醒干预组血浆MT水平低(P<0.05);与睡眠干预组比较,觉醒干预组、睡眠—觉醒干预组血浆MT水平高(P均<0.05)。被干预第6天:各组之间血浆MT水平差异无统计学意义,但呈现出睡眠—觉醒节律干预组>睡眠—觉醒节律未干预组>正常对照组的规律。干预第3天,与正常对照组比较,睡眠—觉醒干预组血浆NE水平高(P<0.05);与睡眠—觉醒节律未干预组、睡眠干预组、觉醒干预组比较,睡眠—觉醒干预组血浆NE水平高(P均<0.05);干预第6天,与正常对照组比较,觉醒干预组血浆NE水平高(P<0.05);与睡眠—觉醒节律未干预组比较,睡眠—觉醒干预组血浆NE水平低(P<0.05);干预第9天:与正常对照组及睡眠干预组比较,睡眠—觉醒干预组NE水平高(P均<0.05)。结论正常睡眠—觉醒节律被干预的再生障碍性贫血小鼠外周血细胞数量升高,血浆MT及NE均升高,以睡眠、觉醒节律均被干预为著;紊乱的睡眠—觉醒节律干预可能通过调节MT、NE水平促使再生障碍性贫血小鼠血细胞数量升高。

Phosphate deficiency induced by infection promotes synthesis of anthracnose-resistant anthocyanin-3-O-galactoside phytoalexins in the Camellia sinensis plant

Tea(Camellia sinensis)is a well-known beverage crop rich in polyphenols with health benefits for humans.Understanding how tea polyphenols participate in plant resistance is beneficial to breeding resistant varieties and uncovering the resistance mechanisms.Here,we report that a Colletotrichum infection-induced‘pink ring’symptom appeared outside the lesion,which is highly likely to occur in resistant cultivars.By identifying morphological feature-specific metabolites in the pink ring and their association with disease resistance,and analysis of the association between metabolite and gene expression,the study revealed that the accumulation of anthocyanin-3-O-galactosides,red phytotoxin compounds resistant to anthracnose,plays a pivotal role in the hypersensitive response surrounding infection sites in tea plants.The results of genetic manipulation showed that the expression of CsF3Ha,CsANSa,CsUGT78A15,CsUGT75L43,and CsMYB113,which are involved in anthocyanin biosynthesis,is positively correlated with anthracnose-resistance and the formation of the pink ring.Further phosphorus quantification and fertilization experiments confirmed that phosphate deficiency caused by anthracnose is involved in the occurrence of the pink ring.Genetic manipulation studies indicated that altering the expression levels of Pi transporter proteins(CsPHT2-1,CsPHT4;4)and phosphate deprivation response transcription factors(CsWRKY75-1,CsWRKY75-2,CsMYB62-1)enhances resistance to anthracnose and the formation of the pink ring symptom in tea plants.This article provides the first evidence that anthocyanin-3-O-galactosides are the anthracnose-resistant phytoalexins among various polyphenols in tea plants,and this presents an approach for identifying resistance genes in tea plants,where genetic transformation is challenging.

Al-induced CsUGT84J2 enhances flavonol and auxin accumulation to promote root growth in tea plants

Although Al is not necessary or even toxic to most plants,it is beneficial for the growth of tea plants.However,the mechanism through which Al promotes root growth in tea plants remains unclear.In the present study,we found that flavonol glycoside levels in tea roots increased following Al treatment,and the Al-induced UDP glycosyltransferase CsUGT84J2 was involved in this mechanism.Enzyme activity assays revealed that rCsUGT84J2 exhibited catalytic activity on multiple types of substrates,including phenolic acids,flavonols,and auxins in vitro.Furthermore,metabolic analysis with UPLC-QqQ-MS/MS revealed significantly increased flavonol and auxin glycoside accumulation in CsUGT84J2-overexpressing Arabidopsis thaliana.In addition,the expression of genes involved in the flavonol pathway as well as in the auxin metabolism,transport,and signaling pathways was remarkably enhanced.Additionally,lateral root growth and exogenous Al stress tolerance were significantly improved in transgenic A.thaliana.Moreover,gene expression and metabolic accumulation related to phenolic acids,flavonols,and auxin were upregulated in CsUGT84J2-overexpressing tea plants but downregulated in CsUGT84J2-silenced tea plants.In conclusion,Al treatment induced CsUGT84J2 expression,mediated flavonol and auxin glycosylation,and regulated endogenous auxin homeostasis in tea roots,thereby promoting the growth of tea plants.Our findings lay the foundation for studying the precise mechanisms through which Al promotes the growth of tea plants.

Functional diversity of subgroup 5 R2R3-MYBs promoting proanthocyanidin biosynthesis and their key residues and motifs in tea plant

The tea plant(Camellia sinensis)is rich in polyphenolic compounds.Particularly,flavan-3-ols and proanthocyanidins(PAs)are essential for the flavor and disease-resistance property of tea leaves.The fifth subgroup of R2R3-MYB transcription factors comprises the primary activators of PA biosynthesis.This study showed that subgroup 5 R2R3-MYBs in tea plants contained at least nine genes belonging to the TT2,MYB5,and MYBPA types.Tannin-rich plants showed an expansion in the number of subgroup 5 R2R3-MYB genes compared with other dicotyledonous and monocot plants.The MYBPA-type genes of tea plant were slightly expanded.qRT–PCR analysis and GUS staining analysis of promoter activity under a series of treatments revealed the differential responses of CsMYB5s to biotic and abiotic stresses.In particular,CsMYB5a,CsMYB5b,and CsMYB5e responded to high-intensity light,high temperature,MeJA,and mechanical wounding,whereas CsMYB5f and CsMYB5g were only induced by wounding.Three genetic transformation systems(C.sinensis,Nicotiana tabacum,and Arabidopsis thaliana)were used to verify the biological function of CsMYB5s.The results show that CsMYB5a,CsMYB5b,and CsMYB5e could promote the gene expression of CsLAR and CsANR.However,CsMYB5f and CsMYB5g could only upregulate the gene expression of CsLAR but not CsANR.A series of site-directed mutation and domain-swapping experiments were used to verify functional domains and key amino acids of CsMYB5s responsible for the regulation of PA biosynthesis.This study aimed to provide insight into the induced expression and functional diversity model of PA biosynthesis regulation in tea plants.

Reoperation for heterochronic intraductal papillary mucinous neoplasm of the pancreas after bile duct neoplasm resection:A case report

BACKGROUND Intraductal papillary neoplasm of the bile duct(IPNB)and intraductal papillary mucinous neoplasm(IPMN)of the pancreas have similar pathological manifestations.However,they often develop separately and it is rare for both to occur together.Patients presenting with heterochronic IPMN after IPNB are prone to be misdiagnosed with tumor recurrence.CASE SUMMARY A 67-year-old male patient was admitted 8.5 years after IPNB carcinoma and 4 years after the discovery of a pancreatic tumor.A left hepatic bile duct tumor with distal bile duct dilatation was found 8.5 years ago by the computed tomography;therefore,a left hepatectomy was performed.The postoperative pathological diagnosis was malignant IPNB with negative cutting edge and pathological stage T1N0M0.Magnetic resonance imaging 4 years ago showed cystic lesions in the pancreatic head with pancreatic duct dilatation,and carcinoembryonic antigen continued to increase.Positron emission tomography showed a maximum standard uptake value of 11.8 in the soft tissue mass in the pancreatic head,and a malignant tumor was considered.Radical pancreatoduodenectomy was performed.Postoperative pathological diagnosis was pancreatic head IPMN with negative cutting edge,pancreaticobiliary type,stage T3N0M0.He was discharged 15 d after the operation.Follow-up for 6 mo showed no tumor recurrence,and quality of life was good.CONCLUSION IPNB and IPMN are precancerous lesions with similar pathological characteristics and require active surgery and long-term follow-up.

特瑞普利单抗致肝损伤伴甲状腺功能减退的处理及监护

目的促进临床安全使用程序性死亡受体-1(PD-1)抑制剂。方法临床药师参与1例使用特瑞普利单抗治疗后同时出现甲状腺功能减退(简称甲减)和免疫性肝炎的鼻咽癌患者的用药监护,通过结合患者用药史、既往史,经会诊并采用诺氏评估量表判别特瑞普利单抗与甲减和肝损伤的关联性,临床药师根据药品不良反应转归结果建议医师调整左甲状腺素钠剂量以改善甲状腺功能,恢复使用抗乙肝病毒药物,加用甲泼尼龙及调整保肝用药方案。结果经判定,患者甲减和肝损伤“很可能”与特瑞普利单抗的使用有关(诺氏评估量表评分为6分)。医师部分采纳临床药师的意见调整用药方案,患者甲减症状得到控制,肝酶水平恢复正常。结论目前,多器官免疫不良反应为特瑞普利单抗在我国的罕见而严重的药品不良反应(ADR)。临床药师应对使用PD-1抑制剂等新型抗肿瘤药物的患者进行全程监护,及时评估ADR并提出药学建议,协助医师完善治疗方案,提高新型抗肿瘤药物临床应用的安全性和有效性。

红霉素对硼替佐米体外和大鼠体内代谢的影响

目的考察红霉素对硼替佐米(BTZ)体外和大鼠体内代谢的影响。方法采用超高效液相色谱串联质谱(UHPLC-MS/MS)法检测体外孵育体系及大鼠血清中BTZ的质量浓度。建立重组人细胞色素P450酶3A4(CYP3A4)的体外反应体系,考察红霉素对BTZ代谢的影响。以酮康唑为阳性对照,将红霉素与BTZ共孵育,CYP3A4浓度为50 pmol/mL,反应30 min,测定BTZ最大反应速率(V_(max)),并求算米氏常数(K_(m))。当BTZ浓度为44.36µmol/L时,根据不同浓度CYP3A4抑制剂(0.05,0.1,0.5,1,2,5,10,20,50µmol/L)作用下BTZ的浓度拟合抑制曲线,计算抑制剂对BTZ的半数抑制浓度(IC_(50))。将30只大鼠随机分为对照组(等量生理盐水)、酮康唑组(32 mg/kg)及红霉素低、中、高剂量组(40,120,240 mg/kg),均尾静脉注射BTZ 0.15 mg/kg,同时分别灌胃相应药物,分别于注射BTZ后5,15,30 min及1.5,2,3,4,6,8,12,24 h时眼眶采血,绘制BTZ在各组大鼠体内消除的平均血药浓度-时间曲线,计算药物代谢动力学参数。结果红霉素及酮康唑对BTZ的体外代谢抑制呈浓度依赖性,IC_(50)分别为0.2298µmol/L和4.8630µmol/L。与对照组相比,红霉素高剂量组和酮康唑组BTZ的C_(max)和AUC_(0-∞)明显增加,分别增加8倍和10倍与11倍和10倍;与对照组相比,红霉素中、低剂量组BTZ的药物代谢动力学参数均显著升高(P<0.05),但两组间无显著差异(P<0.05)。结论红霉素明显抑制CYP3A4活性。240 mg/kg红霉素会增加BTZ的血药浓度,40 mg/kg和120 mg/kg红霉素引起BTZ血药浓度波动的风险较小。

参麻颈复方对脑缺血损伤小鼠的脑保护作用及其机制研究

目的 观察参麻颈复方颗粒对脑缺血损伤小鼠脑组织的保护作用,并探讨其可能机制。方法 将SPF级C57 BL/6雄性小鼠30只随机分为模型对照组、参麻颈组、尼莫地平组,采用电凝法制备小鼠脑缺血损伤动物模型,给参麻颈组、尼莫地平组小鼠灌胃14 d,应用TTC染色法检测各组小鼠脑梗死体积,提取骨髓内皮祖细胞并测定细胞功能,用蛋白免疫印迹法(Western blot)检测内皮祖细胞中蛋白表达水平。结果 与模型对照组比较,参麻颈组小鼠脑梗死体积明显减少,其内皮祖细胞迁移、黏附以及形成小管的能力显著改善,内皮祖细胞中BDNF蛋白表达水平明显升高。结论 参麻颈复方颗粒对脑缺血损伤小鼠脑组织具有保护作用,可能与调节内皮祖细胞中BDNF的表达,改善骨髓来源内皮祖细胞功能密切相关。

“双减”背景下农村小学后进生的行为表现、形成原因及转化策略

随着“双减”政策的实施,农村小学生一定程度上因失去课外管束而呈现后进生数量上升之趋势。如何在“双减”背景下促进农村小学后进生转化,成为班级管理中困扰老师的新问题。本文通过对学生的深入观察和互动,从行为和心理两个层面分析了农村小学班级后进生的表现,并从学生自身、教师、学校管理、家庭教育、社会等角度揭示其成因,提出以适时激励来激发后进生学习内驱力、探索寄宿制办学为后进生提供有效监护、革新作业形式使后进生“扬长避短”、明晰教师惩戒权助力后进生行为习惯矫正等转化策略,从而为转化后进生、提高农村小学生综合素质提供参考。

一起伦敦沙门氏菌食物中毒分离株的病原特征分析

目的分析2020年广州市一起伦敦沙门氏菌食物中毒分离株的病原特征。方法对分离纯化的10株沙门氏菌进行血清型鉴定,耐药检测,毒力岛(Salmonella pathogenicity island, SPI)基因片段SPI-1、SPI-2、SPI-3、SPI-4、SPI-5的PCR检测及脉冲场凝胶电泳法(pulsed-field gel electrophoresis, PFGE)分析。结果 10株沙门氏菌血清型鉴定抗原式均为O10:l、v:1、6,即伦敦沙门氏菌,药敏实验显示10株沙门氏菌对氨苄西林(ampicillin,AMP)、萘啶酸(nalidixicacid,NAL)、头孢唑林(cefazolin,CFZ)、复方磺胺(compound sulfamethoxazole tablets,SXT)、氨苄西林/舒巴坦(ampicillin/sulbactam,AMS)100%耐药,对头孢噻肟(cefotaxime, CTX)、头孢西丁(cefoxitin, CFX)、头孢他啶(ceftazidime, CAZ)、亚胺培南(imipenem, IPM)、庆大霉素(gentamicin,GEN)、四环素(tetracycline,TET)、氯霉素(chloramphenicol,CHL)、环丙沙星(ciprofloxacin,CIP)100%敏感。毒力岛基因SPI-1~SPI-5全部为阳性。脉冲场凝胶电泳法(pulsed field gel electrophoresis, PFGE)聚类分析显示,5株病患株、3株食品株、2株环境株为高度同源株。结论引起此次食物中毒事件的病原菌是伦敦沙门氏菌,应进一步加强餐饮环节的卫生监测和控制。