Objective:To explore the protective effect and underlying mechanism of Lycium barbarum polysaccharides(LBP)in a non-alcoholic fatty liver disease(NAFLD)cell model.Methods:Normal human hepatocyte LO2 cells were treated...Objective:To explore the protective effect and underlying mechanism of Lycium barbarum polysaccharides(LBP)in a non-alcoholic fatty liver disease(NAFLD)cell model.Methods:Normal human hepatocyte LO2 cells were treated with 1 mmol/L free fatty acids(FFA)mixture for 24 h to induce NAFLD cell model.Cells were divided into 5 groups,including control,model,low-,medium-and high dose LBP(30,100and 300μg/mL)groups.The monosaccharide components of LBP were analyzed with high performance liquid chromatography.Effects of LBP on cell viability and intracellular lipid accumulation were assessed by cell counting Kit-8 assay and oil red O staining,respectively.Triglyceride(TG),alanine aminotransferase(ALT),aspartate aminotransferase(AST),adenosine triphosphate(ATP)and oxidative stress indicators were evaluated.Energy balance and mitochondrial biogenesis related mRNA and proteins were determined by quantitative real-time polymerase chain reaction and Western blot,respectively.Results:Heteropolysaccharides with mannose and glucose are the main components of LBP.LBP treatment significantly decreased intracellular lipid accumulation as well as TG,ALT,AST and malondialdehyde levels(P<0.05 or P<0.01),increased the levels of superoxide dismutase,phospholipid hydroperoxide glutathione peroxidase,catalase,and ATP in NAFLD cell model(P<0.05).Meanwhile,the expression of uncoupling protein 2 was down-regulated and peroxisome proliferator-activated receptor gamma coactivator-1α/nuclear respiratory factor 1/mitochondrial transcription factor A pathway was up-regulated(P<0.05).Conclusion:LBP promotes mitochondrial biogenesis and improves energy balance in NAFLD cell model.展开更多
基金Supported by National Natural Science Foundation of China(No.81660537)Special Talents Start-up Project of Ningxia Medical University(No.XT2018009)Natural Science Foundation of Ningxia(No.2020AAC03163)。
文摘Objective:To explore the protective effect and underlying mechanism of Lycium barbarum polysaccharides(LBP)in a non-alcoholic fatty liver disease(NAFLD)cell model.Methods:Normal human hepatocyte LO2 cells were treated with 1 mmol/L free fatty acids(FFA)mixture for 24 h to induce NAFLD cell model.Cells were divided into 5 groups,including control,model,low-,medium-and high dose LBP(30,100and 300μg/mL)groups.The monosaccharide components of LBP were analyzed with high performance liquid chromatography.Effects of LBP on cell viability and intracellular lipid accumulation were assessed by cell counting Kit-8 assay and oil red O staining,respectively.Triglyceride(TG),alanine aminotransferase(ALT),aspartate aminotransferase(AST),adenosine triphosphate(ATP)and oxidative stress indicators were evaluated.Energy balance and mitochondrial biogenesis related mRNA and proteins were determined by quantitative real-time polymerase chain reaction and Western blot,respectively.Results:Heteropolysaccharides with mannose and glucose are the main components of LBP.LBP treatment significantly decreased intracellular lipid accumulation as well as TG,ALT,AST and malondialdehyde levels(P<0.05 or P<0.01),increased the levels of superoxide dismutase,phospholipid hydroperoxide glutathione peroxidase,catalase,and ATP in NAFLD cell model(P<0.05).Meanwhile,the expression of uncoupling protein 2 was down-regulated and peroxisome proliferator-activated receptor gamma coactivator-1α/nuclear respiratory factor 1/mitochondrial transcription factor A pathway was up-regulated(P<0.05).Conclusion:LBP promotes mitochondrial biogenesis and improves energy balance in NAFLD cell model.
