Bacteria in desert soil have unique phylogeny and important ecological functions, and theirresponses to changes in precipitation need further attention. However, relevant studies have mainlyfocused on the surface soil...Bacteria in desert soil have unique phylogeny and important ecological functions, and theirresponses to changes in precipitation need further attention. However, relevant studies have mainlyfocused on the surface soil, and studies on the responses of bacteria at different soil depths to variationsin precipitation are rare. Thus, we used 16S rDNA high-throughput sequencing to investigate the changesin soil bacterial distribution along a mean annual precipitation gradient (50–150 mm) in the Alxa Desert,China, and compared the variation characteristics in the surface soil layer (0–10 cm) and subsurface soillayer (10–20 cm). Results showed that soil bacterial communities significantly changed along theprecipitation gradient in both soil layers. However, the subsurface soil layer could support bacterialcommunities with higher diversity and closer internal relationships but more internal competition than thesurface soil layer. Additionally, compared with the surface soil layer, variations in diversity andco-occurrence patterns in the subsurface soil layer were more in line with the changes in the mean annualprecipitation, while bacterial community structure was less variable in the subsurface soil layer. Comparedwith the mean annual precipitation, soil moisture had little influence on the structure and diversity of soilbacterial community but had a high correlation with intercommunity connectivity. Therefore, soilmoisture might play a complex role in mediating environmental conditions and soil bacterial communitycharacteristics. Due to the different responses of surface and subsurface soil bacteria to the changes inprecipitation, it is necessary to distinguish different soil layers when predicting the trends in desert soilbacterial conditions associated with precipitation, and prediction of subsurface soil bacteria may be moreaccurate.展开更多
基金This work was financially supported by the National Key Research and Development Program of China(2016YFC0501001)the Key Laboratory Cooperative Research Project of Chinese Academy of Sciences.
文摘Bacteria in desert soil have unique phylogeny and important ecological functions, and theirresponses to changes in precipitation need further attention. However, relevant studies have mainlyfocused on the surface soil, and studies on the responses of bacteria at different soil depths to variationsin precipitation are rare. Thus, we used 16S rDNA high-throughput sequencing to investigate the changesin soil bacterial distribution along a mean annual precipitation gradient (50–150 mm) in the Alxa Desert,China, and compared the variation characteristics in the surface soil layer (0–10 cm) and subsurface soillayer (10–20 cm). Results showed that soil bacterial communities significantly changed along theprecipitation gradient in both soil layers. However, the subsurface soil layer could support bacterialcommunities with higher diversity and closer internal relationships but more internal competition than thesurface soil layer. Additionally, compared with the surface soil layer, variations in diversity andco-occurrence patterns in the subsurface soil layer were more in line with the changes in the mean annualprecipitation, while bacterial community structure was less variable in the subsurface soil layer. Comparedwith the mean annual precipitation, soil moisture had little influence on the structure and diversity of soilbacterial community but had a high correlation with intercommunity connectivity. Therefore, soilmoisture might play a complex role in mediating environmental conditions and soil bacterial communitycharacteristics. Due to the different responses of surface and subsurface soil bacteria to the changes inprecipitation, it is necessary to distinguish different soil layers when predicting the trends in desert soilbacterial conditions associated with precipitation, and prediction of subsurface soil bacteria may be moreaccurate.
