Polygala paniculata L.is a medicinal plant that grows in the Brazilian Atlantic coast,known as‘barba-de-São-João’,‘barba-de-bode’,‘vassourinha branca’,and‘mimosa’.In this study,pollen viability was e...Polygala paniculata L.is a medicinal plant that grows in the Brazilian Atlantic coast,known as‘barba-de-São-João’,‘barba-de-bode’,‘vassourinha branca’,and‘mimosa’.In this study,pollen viability was estimated by three different staining methods:2%acetic orcein,2%acetic carmine,and Alexander’s stain.The young inflorescences of twenty accessions were collected and fixed in a solution of ethanol:acetic acid(3:1)for 24 hours,then stored in ethanol 70%under refrigeration.Six slides per plant,two for each stain,were prepared by squashing,and 300 pollen grains per slide were analyzed.Pollen viability was high(>70%)for most accessions of P.paniculata using the Alexander’s stain,which proved the most adequate method to estimate pollen viability.展开更多
Field collected roots of four populations of Sida rhombifolia were used for preparing aqueous de-coctions at two concentrations:4g/L;and 16g/L.Afterwards,we used three groups of six onion(Allium cepa)bulbs for testing...Field collected roots of four populations of Sida rhombifolia were used for preparing aqueous de-coctions at two concentrations:4g/L;and 16g/L.Afterwards,we used three groups of six onion(Allium cepa)bulbs for testing each population.Slides were made with all bulbs through the smashing technique.Cells in all phases of the cell cycle of A.cepa were analyzed.The mitotic index(%of cells in mitosis)was calculated,and the statistical analysis through theχ2 test was carried out at 5%probability.The results showed that the aqueous extracts of S.rhombifolia have antiproliferative activity at high concentrations.Practically no chromosomal ab-errations were induced by treatments.展开更多
文摘Polygala paniculata L.is a medicinal plant that grows in the Brazilian Atlantic coast,known as‘barba-de-São-João’,‘barba-de-bode’,‘vassourinha branca’,and‘mimosa’.In this study,pollen viability was estimated by three different staining methods:2%acetic orcein,2%acetic carmine,and Alexander’s stain.The young inflorescences of twenty accessions were collected and fixed in a solution of ethanol:acetic acid(3:1)for 24 hours,then stored in ethanol 70%under refrigeration.Six slides per plant,two for each stain,were prepared by squashing,and 300 pollen grains per slide were analyzed.Pollen viability was high(>70%)for most accessions of P.paniculata using the Alexander’s stain,which proved the most adequate method to estimate pollen viability.
文摘Field collected roots of four populations of Sida rhombifolia were used for preparing aqueous de-coctions at two concentrations:4g/L;and 16g/L.Afterwards,we used three groups of six onion(Allium cepa)bulbs for testing each population.Slides were made with all bulbs through the smashing technique.Cells in all phases of the cell cycle of A.cepa were analyzed.The mitotic index(%of cells in mitosis)was calculated,and the statistical analysis through theχ2 test was carried out at 5%probability.The results showed that the aqueous extracts of S.rhombifolia have antiproliferative activity at high concentrations.Practically no chromosomal ab-errations were induced by treatments.
