The present study was performed mainly to investigate the antagonist-pathogen-host interaction in wounds of the sweet cherry fruits. The antagonistic yeast Cryptococcus laurentii could significantly reduce the brown r...The present study was performed mainly to investigate the antagonist-pathogen-host interaction in wounds of the sweet cherry fruits. The antagonistic yeast Cryptococcus laurentii could significantly reduce the brown rot of the sweet cherry fruit caused by Monilinia fructicola at 25 and 1 ℃. The populations of yeast increased faster in the presence of the pathogen initially, but then decreased rapidly. In the fruits inoculated with M. fructicola alone or combined with C. laurentii, an induction of lipid peroxidation as well as activities of the antioxidant enzymes, such as, superoxide dismutases （SOD）, catalase （CAT）, and peroxidase （POD）, was observed. The isoenzyme pattern of polypheno/oxidase （PPO） changed greatly after the symptoms appeared, with new PPO isoforms being induced. By contrast, the induction of lipid peroxidation and activities of SOD, CAT, and POD were low, although no significant changes were found in the PPO isoenzyms in the fruits inoculated with antagonist C. laurentii alone. The inhibition of brown rot during the antagonist- pathogen-host interaction in wounds of the sweet cherry fruits was mainly on account of the stimulated growth of C. laurentii as well as the induction of antioxidant enzymes of the fruits by M. fructicola.展开更多
The effect of boron on the properties of Konjac Glucomanan （KGM） has been investigated by the method of experiment and molecular dynamic simulation. Upon analysis, the property and structure of KGM are apt to be aff...The effect of boron on the properties of Konjac Glucomanan （KGM） has been investigated by the method of experiment and molecular dynamic simulation. Upon analysis, the property and structure of KGM are apt to be affected by boron and structural reasons for property change were discussed. In detail, the addition low concentration borax can increase the systematic inherent viscosity, by contrast, high concentration borax has opposite effect on the viscosity. When adding borax, the micropores on KGM film surface decrease or disappear, leading to more compact and uniform on the film surface. The structure of KGM-Boron complex is described as the coordination reaction between KGM and boron. The main reaction points are hydroxyl group on C（6） position of sugar as well as those on C（2） and C（3） positions of mannose with two kinds of complexes formation： B-K2 and KB-K. And KB-K mainly consists of g-b-m.展开更多
基金the Knowledge Innovation Program of the Chinese Academy of Sciences, China （KSCX2-YW-G-010）； the National Natural Science Foundation of China （30671473）.
文摘The present study was performed mainly to investigate the antagonist-pathogen-host interaction in wounds of the sweet cherry fruits. The antagonistic yeast Cryptococcus laurentii could significantly reduce the brown rot of the sweet cherry fruit caused by Monilinia fructicola at 25 and 1 ℃. The populations of yeast increased faster in the presence of the pathogen initially, but then decreased rapidly. In the fruits inoculated with M. fructicola alone or combined with C. laurentii, an induction of lipid peroxidation as well as activities of the antioxidant enzymes, such as, superoxide dismutases （SOD）, catalase （CAT）, and peroxidase （POD）, was observed. The isoenzyme pattern of polypheno/oxidase （PPO） changed greatly after the symptoms appeared, with new PPO isoforms being induced. By contrast, the induction of lipid peroxidation and activities of SOD, CAT, and POD were low, although no significant changes were found in the PPO isoenzyms in the fruits inoculated with antagonist C. laurentii alone. The inhibition of brown rot during the antagonist- pathogen-host interaction in wounds of the sweet cherry fruits was mainly on account of the stimulated growth of C. laurentii as well as the induction of antioxidant enzymes of the fruits by M. fructicola.
基金This work was supported by the National Natural Foundation (30371009), Foundation of Education Committee of Fujian Province(JA03059) and Key Science & Technology Item of Fujian Province (2003Y008).
文摘The effect of boron on the properties of Konjac Glucomanan （KGM） has been investigated by the method of experiment and molecular dynamic simulation. Upon analysis, the property and structure of KGM are apt to be affected by boron and structural reasons for property change were discussed. In detail, the addition low concentration borax can increase the systematic inherent viscosity, by contrast, high concentration borax has opposite effect on the viscosity. When adding borax, the micropores on KGM film surface decrease or disappear, leading to more compact and uniform on the film surface. The structure of KGM-Boron complex is described as the coordination reaction between KGM and boron. The main reaction points are hydroxyl group on C（6） position of sugar as well as those on C（2） and C（3） positions of mannose with two kinds of complexes formation： B-K2 and KB-K. And KB-K mainly consists of g-b-m.
