目的探讨乳腺癌摄取^(99)Tc^(m)-甲氧异腈(MIBI)与Ki-67抗原表达的关系,并验证Ki-67预测新辅助化疗(NAC)疗效的价值。方法回顾性分析2018年1月至2020年3月于宁夏医科大学总医院就诊的47例乳腺癌患者NAC前后双时相^(99)Tc^(m)-MIBI SPECT...目的探讨乳腺癌摄取^(99)Tc^(m)-甲氧异腈(MIBI)与Ki-67抗原表达的关系,并验证Ki-67预测新辅助化疗(NAC)疗效的价值。方法回顾性分析2018年1月至2020年3月于宁夏医科大学总医院就诊的47例乳腺癌患者NAC前后双时相^(99)Tc^(m)-MIBI SPECT/CT断层融合显像,分析T/N比值和肿瘤大小在治疗前后的变化及与Ki-67之间的关系。采用两样本t检验、直线相关性及χ^(2)检验分析数据。结果乳腺癌^(99)Tc^(m)-MIBI显像NAC前后20 min T/N比值、2 h T/N比值下降差异有统计意义(P<0.05),肿瘤NAC化疗前后最大径缩小差异有统计学意义(P<0.05);Ki-67高表达组与低表达组患者NAC后T/N比值下降和肿瘤缩小差异均有统计学意义(P<0.05);Ki-67的表达与20 min和2 h T/N比值有相关性(P<0.05)。结论Ki-67抗原表达和乳腺^(99)Tc^(m)-MIBI显像有助于了解肿瘤增殖,Ki-67抗原表达对NAC疗效有预测价值,可用于指导临床治疗。展开更多
Objective To investigate the mechanism of Tojapride,a Chinese herbal formula extract,on strengthening the barrier function of esophageal epithelium in rats with reflux esophagitis(RE).Methods Ten out of 85 SD rats wer...Objective To investigate the mechanism of Tojapride,a Chinese herbal formula extract,on strengthening the barrier function of esophageal epithelium in rats with reflux esophagitis(RE).Methods Ten out of 85 SD rats were randomly selected as the sham group(n10),and 75 rats were developed a reflux esophagitis model(RE)by the esophageal and duodenal side-to-side anastomosis.Fifty successful modeling rats were divided into different medicated groups through a random number table including the model,low-,medium-,and high-dose of Tojapride as well as omeprazole groups(n10).Three doses of Tojapride[5.73,11.46,22.92 g/(kg•d)]and omeprazole[4.17 mg/(kg•d)]were administrated intragastrically twice daily for 3 weeks.And the rats in the sham and model groups were administered 10 mL/kg distilled water.Gastric fluid was collected and the supernatant was kept to measure for volume,pH value and acidity.Esophageal tissues were isolated to monitor the morphological changes through hematoxylin-eosin(HE)staining,and esophageal epithelial ultrastructure was observed by transmission electron microscopy.The expressions of nuclear factor kappa-light-chain-enhancer of activated B cells p65(NF-KBp65),κB kinase beta(IKKß),occludin,and zonula occludens-1(ZO-1)in the esophageal tissues were measured by immunohistochemistry and Western blot,respectively.Results The gastric pH value in the model group was significantly lower than the sham group(P<0.05).Compared with the model group,gastric pH value in the omeprazole and medium-dose of Tojapride groups were significantly higher(P<0.05).A large area of ulceration was found on the esophageal mucosa from the model rats,while varying degrees of congestion and partially visible erosion was observed in the remaining groups.Remarkable increase in cell gap width and decrease in desmosome count was seen in RE rats and the effect was reversed by Tojapride treatment.Compared with the sham group,the IKKßlevels were significantly higher in the model group(P<0.05).However,the IKKßlevels were down-regulated after treatment by all doses of Tojapride(P<0.01 or P<0.05).The occluding and ZO-1 levels decreased in the model group compared with the sham group(Ps0.01 or Ps0.05),while both indices were significantly up-regulated in the Tojapride-treated groups(P<0.01 or P<0.05).Conclusions Tojapride could improve the pathological conditions of esophageal epithelium in RE rats.The underlying mechanisms may involve in down-regulating the IKKßexpression and elevating ZO-1 and occludin expression,thereby alleviating the inflammation of the esophagus and strengthening the barrier function of the esophageal epithelium.展开更多
文摘目的探讨乳腺癌摄取^(99)Tc^(m)-甲氧异腈(MIBI)与Ki-67抗原表达的关系,并验证Ki-67预测新辅助化疗(NAC)疗效的价值。方法回顾性分析2018年1月至2020年3月于宁夏医科大学总医院就诊的47例乳腺癌患者NAC前后双时相^(99)Tc^(m)-MIBI SPECT/CT断层融合显像,分析T/N比值和肿瘤大小在治疗前后的变化及与Ki-67之间的关系。采用两样本t检验、直线相关性及χ^(2)检验分析数据。结果乳腺癌^(99)Tc^(m)-MIBI显像NAC前后20 min T/N比值、2 h T/N比值下降差异有统计意义(P<0.05),肿瘤NAC化疗前后最大径缩小差异有统计学意义(P<0.05);Ki-67高表达组与低表达组患者NAC后T/N比值下降和肿瘤缩小差异均有统计学意义(P<0.05);Ki-67的表达与20 min和2 h T/N比值有相关性(P<0.05)。结论Ki-67抗原表达和乳腺^(99)Tc^(m)-MIBI显像有助于了解肿瘤增殖,Ki-67抗原表达对NAC疗效有预测价值,可用于指导临床治疗。
基金Supported by Natural Science Foundation of China(No.81503560)“Ten Diseases and Ten Drugs”Program and the Cultivation Research of Biological Medicine and Life Science Innovation of Beijing Municipal Science and Technology Commission(No.Z141100002214012,Z161100000116046)。
文摘Objective To investigate the mechanism of Tojapride,a Chinese herbal formula extract,on strengthening the barrier function of esophageal epithelium in rats with reflux esophagitis(RE).Methods Ten out of 85 SD rats were randomly selected as the sham group(n10),and 75 rats were developed a reflux esophagitis model(RE)by the esophageal and duodenal side-to-side anastomosis.Fifty successful modeling rats were divided into different medicated groups through a random number table including the model,low-,medium-,and high-dose of Tojapride as well as omeprazole groups(n10).Three doses of Tojapride[5.73,11.46,22.92 g/(kg•d)]and omeprazole[4.17 mg/(kg•d)]were administrated intragastrically twice daily for 3 weeks.And the rats in the sham and model groups were administered 10 mL/kg distilled water.Gastric fluid was collected and the supernatant was kept to measure for volume,pH value and acidity.Esophageal tissues were isolated to monitor the morphological changes through hematoxylin-eosin(HE)staining,and esophageal epithelial ultrastructure was observed by transmission electron microscopy.The expressions of nuclear factor kappa-light-chain-enhancer of activated B cells p65(NF-KBp65),κB kinase beta(IKKß),occludin,and zonula occludens-1(ZO-1)in the esophageal tissues were measured by immunohistochemistry and Western blot,respectively.Results The gastric pH value in the model group was significantly lower than the sham group(P<0.05).Compared with the model group,gastric pH value in the omeprazole and medium-dose of Tojapride groups were significantly higher(P<0.05).A large area of ulceration was found on the esophageal mucosa from the model rats,while varying degrees of congestion and partially visible erosion was observed in the remaining groups.Remarkable increase in cell gap width and decrease in desmosome count was seen in RE rats and the effect was reversed by Tojapride treatment.Compared with the sham group,the IKKßlevels were significantly higher in the model group(P<0.05).However,the IKKßlevels were down-regulated after treatment by all doses of Tojapride(P<0.01 or P<0.05).The occluding and ZO-1 levels decreased in the model group compared with the sham group(Ps0.01 or Ps0.05),while both indices were significantly up-regulated in the Tojapride-treated groups(P<0.01 or P<0.05).Conclusions Tojapride could improve the pathological conditions of esophageal epithelium in RE rats.The underlying mechanisms may involve in down-regulating the IKKßexpression and elevating ZO-1 and occludin expression,thereby alleviating the inflammation of the esophagus and strengthening the barrier function of the esophageal epithelium.
